成年小鼠大脑皮层中截短 TrkB 的定位以及与全长 TrkB 的共表达

IF 2.5 3区 医学 Q3 ENDOCRINOLOGY & METABOLISM
Koji Ohira
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引用次数: 0

摘要

脑源性神经营养因子(BDNF)是神经营养素之一,其特异性受体TrkB大量分布于中枢神经系统(CNS),具有神经存活、神经元伸长、神经分化和增强突触功能等多种生物学效应。目前,TrkB 有两种亚型:全长 TrkB(TrkB-FL)和 TrkB-T1,前者细胞内结构域含有酪氨酸激酶,后者则是酪氨酸激酶缺陷型。TrkB-FL是典型的酪氨酸激酶受体,而TrkB-T1则是在成年哺乳动物中枢神经系统中表达的主要形式,但其功能尚不清楚。在这项研究中,我们使用 TrkB-T1 抗血清和多种神经元和胶质细胞标记分子抗体对成年小鼠的大脑皮层进行了荧光染色。我们发现,TrkB-T1 不仅在神经元中表达,在星形胶质细胞中也有表达。相反,TrkB-T1 在少突胶质细胞和小胶质细胞中几乎没有表达。几乎所有表达 TrkB-FL 的细胞都表达了 TrkB-T1,这表明 TrkB 亚型之间存在直接相互作用。这些发现表明,BDNF-TrkB 信号转导的各种功能的一部分可能是由于 TrkB 亚型在大脑皮层中的相互作用和细胞定位。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Localization of truncated TrkB and co-expression with full-length TrkB in the cerebral cortex of adult mice

Brain-derived neurotrophic factor (BDNF), one of the neurotrophins, and its specific receptor TrkB, are abundantly distributed in the central nervous system (CNS) and have a variety of biological effects, such as neural survival, neurite elongation, neural differentiation, and enhancing synaptic functions. Currently, there are two TrkB subtypes: full-length TrkB (TrkB-FL), which has a tyrosine kinase in the intracellular domain, and TrkB-T1, which is a tyrosine kinase-deficient form. While TrkB-FL is a typical tyrosine kinase receptor, TrkB-T1 is a main form expressed in the CNS of adult mammals, but its function is unknown. In this study, we performed fluorescent staining of the cerebral cortex of adult mice, by using TrkB-T1 antiserum and various antibodies of marker molecules for neurons and glial cells. We found that TrkB-T1 was expressed not only in neurons but also in astrocytes. In contrast, little expression of TrkB-T1 was found in oligodendrocytes and microglia. TrkB-T1 was expressed in almost all of the cells expressing TrkB-FL, indicating the direct interaction between TrkB subtypes. These findings suggest that a part of various functions of BDNF-TrkB signaling might be due to the interaction and cellular localization of TrkB subtypes in the cerebral cortex.

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来源期刊
Neuropeptides
Neuropeptides 医学-内分泌学与代谢
CiteScore
5.40
自引率
6.90%
发文量
55
审稿时长
>12 weeks
期刊介绍: The aim of Neuropeptides is the rapid publication of original research and review articles, dealing with the structure, distribution, actions and functions of peptides in the central and peripheral nervous systems. The explosion of research activity in this field has led to the identification of numerous naturally occurring endogenous peptides which act as neurotransmitters, neuromodulators, or trophic factors, to mediate nervous system functions. Increasing numbers of non-peptide ligands of neuropeptide receptors have been developed, which act as agonists or antagonists in peptidergic systems. The journal provides a unique opportunity of integrating the many disciplines involved in all neuropeptide research. The journal publishes articles on all aspects of the neuropeptide field, with particular emphasis on gene regulation of peptide expression, peptide receptor subtypes, transgenic and knockout mice with mutations in genes for neuropeptides and peptide receptors, neuroanatomy, physiology, behaviour, neurotrophic factors, preclinical drug evaluation, clinical studies, and clinical trials.
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