二氧化硅纳米颗粒增强了用于血管组织再生的多层细胞外基质支架的细胞和血液相容性。

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biotechnology Letters Pub Date : 2024-04-01 Epub Date: 2024-01-27 DOI:10.1007/s10529-023-03459-8
Leslie A Goldberg, Helena D Zomer, Calum McFetridge, Peter S McFetridge
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引用次数: 0

摘要

目的:用于血管搭桥手术的自体血管有限,是治疗严重心血管疾病的主要障碍。基于这一临床重点,我们的研究小组通过将人羊膜卷成多层细胞外基质(ECM),开发出一种新型工程血管移植物。经二氧化硅纳米颗粒(SiNP)处理后,这些滚动支架的结构和机械性能得到显著改善,与金标准自体移植物的结构和机械性能相当。然而,细胞对 SiNP 处理过的材料有何反应仍有待确定。作为了解添加了 SiNP 的生物材料生物相容性的第一步,我们旨在评估内皮细胞和血液成分如何与 SiNP 处理过的 ECM 支架相互作用:为了验证这一点,我们使用已建立的体外试验来研究 SiNP 和 SiNP 处理过的支架的细胞相容性和血液相容性:结果:我们的研究结果表明,SiNP 对细胞的影响是浓度依赖性的,在 10 μg/ml 以下的 SiNP 浓度不会对细胞产生不良影响,而更高浓度的 SiNP 会诱导细胞毒性和溶血反应。SiNP 还增强了支架的疏水性,众所周知,疏水性可抑制血小板和免疫细胞的粘附。因此,经 SiNP 处理的支架还能支持内皮细胞生长,同时防止血小板和白细胞粘附:我们的研究结果表明,在人羊膜细胞外基质中添加 SiNP 可改善轧制支架的细胞相容性和血液相容性,并强调了这一策略是稳定分层胶原支架用于血管组织再生的可靠机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Silica nanoparticles enhance the cyto- and hemocompatibility of a multilayered extracellular matrix scaffold for vascular tissue regeneration.

Silica nanoparticles enhance the cyto- and hemocompatibility of a multilayered extracellular matrix scaffold for vascular tissue regeneration.

Purpose: The limited availability of autologous vessels for vascular bypass surgeries is a major roadblock to treating severe cardiovascular diseases. Based on this clinical priority, our group has developed a novel engineered vascular graft by rolling human amniotic membranes into multilayered extracellular matrixes (ECM). When treated with silica nanoparticles (SiNP), these rolled scaffolds showed a significant improvement in their structural and mechanical properties, matching those from gold standard autologous grafts. However, it remained to be determined how cells respond to SiNP-treated materials. As a first step toward understanding the biocompatibility of SiNP-dosed biomaterials, we aimed to assess how endothelial cells and blood components interact with SiNP-treated ECM scaffolds.

Methods: To test this, we used established in vitro assays to study SiNP and SiNP-treated scaffolds' cyto and hemocompatibility.

Results: Our results showed that SiNP effects on cells were concentration-dependent with no adverse effects observed up to 10 μg/ml of SiNP, with higher concentrations inducing cytotoxic and hemolytic responses. The SiNP also enhanced the scaffold's hydrophobicity state, a feature known to inhibit platelet and immune cell adhesion. Accordingly, SiNP-treated scaffolds were also shown to support endothelial cell growth while preventing platelet and leukocyte adhesion.

Conclusion: Our findings suggest that the addition of SiNP to human amniotic membrane extracellular matrixes improves the cyto- and hemocompatibility of rolled scaffolds and highlights this strategy as a robust mechanism to stabilize layered collagen scaffolds for vascular tissue regeneration.

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来源期刊
Biotechnology Letters
Biotechnology Letters 工程技术-生物工程与应用微生物
CiteScore
5.90
自引率
3.70%
发文量
108
审稿时长
1.2 months
期刊介绍: Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.
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