Linmeng Tang , Dongbin Chen , Dehong Yang , Zhiwei Liu , Xu Yang , Yujia Liu , Liying Zhang , Zulian Liu , Yaohui Wang , Zheng Tang , Yongping Huang
{"title":"Bmpali、Bmb1 和 Bmcap 是桑蚕尿酸颗粒形成所必需的。","authors":"Linmeng Tang , Dongbin Chen , Dehong Yang , Zhiwei Liu , Xu Yang , Yujia Liu , Liying Zhang , Zulian Liu , Yaohui Wang , Zheng Tang , Yongping Huang","doi":"10.1016/j.ibmb.2024.104075","DOIUrl":null,"url":null,"abstract":"<div><p>Uric acid is the end-product of nitrogen metabolism of the silkworm and other lepidopterans. The accumulation of uric acid particles in the epidermis causes the larval silkworm to appear white and opaque. However, the mechanism of uric acid granule formation is still unclear. Silkworm epidermis color is linked to the genes responsible for uric acid particle formation. We first identified two genes in the <em>Bombyx mori</em> genome that encode subunits of the Bloc-1 (Biogenesis of Lysosome-related Organelles Complex-1) by homology to these genes in other eukaryotes, <em>Bmpali</em> and <em>Bmb1</em>. Mutation in these genes caused a transparent phenotype in the silkworm larvae, and the loss of BmBloc-1 subunit gene <em>Bmcap</em> resulted in the same phenotype. These three genes are highly conserved between human and silkworm. We discovered that <em>Bmpali</em>, <em>Bmcap</em>, and <em>Bmb1</em> localize in the cytoplasm of BmN cells. Yeast two-hybrid assays demonstrated that the Bmpali physically interacts with both Bmcap and Bmb1. Investigating the roles of <em>Bmpali</em>, <em>Bmb1</em>, and <em>Bmcap</em> is essential for uric acid granule formation understanding in <em>Bombyx mori</em>. These mutants present a valuable silkworm model for studying the biogenesis of lysosome-related organelles (LROs).</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"167 ","pages":"Article 104075"},"PeriodicalIF":3.2000,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bmpali, Bmb1 and Bmcap are necessary for uric acid granule formation in Bombyx mori\",\"authors\":\"Linmeng Tang , Dongbin Chen , Dehong Yang , Zhiwei Liu , Xu Yang , Yujia Liu , Liying Zhang , Zulian Liu , Yaohui Wang , Zheng Tang , Yongping Huang\",\"doi\":\"10.1016/j.ibmb.2024.104075\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Uric acid is the end-product of nitrogen metabolism of the silkworm and other lepidopterans. The accumulation of uric acid particles in the epidermis causes the larval silkworm to appear white and opaque. However, the mechanism of uric acid granule formation is still unclear. Silkworm epidermis color is linked to the genes responsible for uric acid particle formation. We first identified two genes in the <em>Bombyx mori</em> genome that encode subunits of the Bloc-1 (Biogenesis of Lysosome-related Organelles Complex-1) by homology to these genes in other eukaryotes, <em>Bmpali</em> and <em>Bmb1</em>. Mutation in these genes caused a transparent phenotype in the silkworm larvae, and the loss of BmBloc-1 subunit gene <em>Bmcap</em> resulted in the same phenotype. These three genes are highly conserved between human and silkworm. We discovered that <em>Bmpali</em>, <em>Bmcap</em>, and <em>Bmb1</em> localize in the cytoplasm of BmN cells. Yeast two-hybrid assays demonstrated that the Bmpali physically interacts with both Bmcap and Bmb1. Investigating the roles of <em>Bmpali</em>, <em>Bmb1</em>, and <em>Bmcap</em> is essential for uric acid granule formation understanding in <em>Bombyx mori</em>. These mutants present a valuable silkworm model for studying the biogenesis of lysosome-related organelles (LROs).</p></div>\",\"PeriodicalId\":330,\"journal\":{\"name\":\"Insect Biochemistry and Molecular Biology\",\"volume\":\"167 \",\"pages\":\"Article 104075\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2024-01-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Insect Biochemistry and Molecular Biology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0965174824000067\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Insect Biochemistry and Molecular Biology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0965174824000067","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Bmpali, Bmb1 and Bmcap are necessary for uric acid granule formation in Bombyx mori
Uric acid is the end-product of nitrogen metabolism of the silkworm and other lepidopterans. The accumulation of uric acid particles in the epidermis causes the larval silkworm to appear white and opaque. However, the mechanism of uric acid granule formation is still unclear. Silkworm epidermis color is linked to the genes responsible for uric acid particle formation. We first identified two genes in the Bombyx mori genome that encode subunits of the Bloc-1 (Biogenesis of Lysosome-related Organelles Complex-1) by homology to these genes in other eukaryotes, Bmpali and Bmb1. Mutation in these genes caused a transparent phenotype in the silkworm larvae, and the loss of BmBloc-1 subunit gene Bmcap resulted in the same phenotype. These three genes are highly conserved between human and silkworm. We discovered that Bmpali, Bmcap, and Bmb1 localize in the cytoplasm of BmN cells. Yeast two-hybrid assays demonstrated that the Bmpali physically interacts with both Bmcap and Bmb1. Investigating the roles of Bmpali, Bmb1, and Bmcap is essential for uric acid granule formation understanding in Bombyx mori. These mutants present a valuable silkworm model for studying the biogenesis of lysosome-related organelles (LROs).
期刊介绍:
This international journal publishes original contributions and mini-reviews in the fields of insect biochemistry and insect molecular biology. Main areas of interest are neurochemistry, hormone and pheromone biochemistry, enzymes and metabolism, hormone action and gene regulation, gene characterization and structure, pharmacology, immunology and cell and tissue culture. Papers on the biochemistry and molecular biology of other groups of arthropods are published if of general interest to the readership. Technique papers will be considered for publication if they significantly advance the field of insect biochemistry and molecular biology in the opinion of the Editors and Editorial Board.