H. Ghaoui , I. Bitam , S. Zaidi , N. Achour , S. Zenia , T. Idres , P.E. Fournier
{"title":"阿尔及利亚北部反刍动物和流浪猫狗中烧伤柯西氏杆菌的分子检测和 MST 基因分型","authors":"H. Ghaoui , I. Bitam , S. Zaidi , N. Achour , S. Zenia , T. Idres , P.E. Fournier","doi":"10.1016/j.cimid.2024.102126","DOIUrl":null,"url":null,"abstract":"<div><p>Aiming at identifying the reservoir and contamination sources of <em>Coxiella burnetii</em> in Northern Algeria, we investigated the molecular presence of the bacterium in 599 samples (blood, placenta, liver, spleen, and uterus) collected from cattle, sheep, dogs and cats. Our qPCR results showed that <strong>15/344 (4.36%)</strong> blood samples and <strong>six/255 (2.35%)</strong> organ specimens were positive for <em>C. burnetii</em>. In cattle, <strong>three (4%)</strong> blood and liver samples were positive. In sheep, one blood <strong>(1.19%)</strong> and <strong>3 (8.57%)</strong> placenta samples were positive. At the Algiers dog pound, <strong>8 (10%)</strong> and <strong>3 (5%)</strong> blood samples were qPCR positivein dogs and cats, respectively. In addition, <strong>MST</strong> genotyping showed that <strong>MST 33</strong> was present in cattle and sheep, <strong>MST 20</strong> in cattle,and<strong>MST 21</strong> in dogs and cats.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"106 ","pages":"Article 102126"},"PeriodicalIF":2.0000,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular detection and MST genotyping of Coxiella burnetii in ruminants and stray dogs and cats in Northern Algeria\",\"authors\":\"H. Ghaoui , I. Bitam , S. Zaidi , N. Achour , S. Zenia , T. Idres , P.E. Fournier\",\"doi\":\"10.1016/j.cimid.2024.102126\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Aiming at identifying the reservoir and contamination sources of <em>Coxiella burnetii</em> in Northern Algeria, we investigated the molecular presence of the bacterium in 599 samples (blood, placenta, liver, spleen, and uterus) collected from cattle, sheep, dogs and cats. Our qPCR results showed that <strong>15/344 (4.36%)</strong> blood samples and <strong>six/255 (2.35%)</strong> organ specimens were positive for <em>C. burnetii</em>. In cattle, <strong>three (4%)</strong> blood and liver samples were positive. In sheep, one blood <strong>(1.19%)</strong> and <strong>3 (8.57%)</strong> placenta samples were positive. At the Algiers dog pound, <strong>8 (10%)</strong> and <strong>3 (5%)</strong> blood samples were qPCR positivein dogs and cats, respectively. In addition, <strong>MST</strong> genotyping showed that <strong>MST 33</strong> was present in cattle and sheep, <strong>MST 20</strong> in cattle,and<strong>MST 21</strong> in dogs and cats.</p></div>\",\"PeriodicalId\":50999,\"journal\":{\"name\":\"Comparative Immunology Microbiology and Infectious Diseases\",\"volume\":\"106 \",\"pages\":\"Article 102126\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-01-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative Immunology Microbiology and Infectious Diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0147957124000031\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Immunology Microbiology and Infectious Diseases","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0147957124000031","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Molecular detection and MST genotyping of Coxiella burnetii in ruminants and stray dogs and cats in Northern Algeria
Aiming at identifying the reservoir and contamination sources of Coxiella burnetii in Northern Algeria, we investigated the molecular presence of the bacterium in 599 samples (blood, placenta, liver, spleen, and uterus) collected from cattle, sheep, dogs and cats. Our qPCR results showed that 15/344 (4.36%) blood samples and six/255 (2.35%) organ specimens were positive for C. burnetii. In cattle, three (4%) blood and liver samples were positive. In sheep, one blood (1.19%) and 3 (8.57%) placenta samples were positive. At the Algiers dog pound, 8 (10%) and 3 (5%) blood samples were qPCR positivein dogs and cats, respectively. In addition, MST genotyping showed that MST 33 was present in cattle and sheep, MST 20 in cattle,andMST 21 in dogs and cats.
期刊介绍：
Comparative Immunology, Microbiology & Infectious Diseases aims to respond to the concept of "One Medicine" and to provide a venue for scientific exchange. Based on the concept of "Comparative Medicine" interdisciplinary cooperation between specialists in human and animal medicine is of mutual interest and benefit. Therefore, there is need to combine the respective interest of physicians, veterinarians and other health professionals for comparative studies relevant to either human or animal medicine .
The journal is open to subjects of common interest related to the immunology, immunopathology, microbiology, parasitology and epidemiology of human and animal infectious diseases, especially zoonotic infections, and animal models of human infectious diseases. The role of environmental factors in disease emergence is emphasized. CIMID is mainly focusing on applied veterinary and human medicine rather than on fundamental experimental research.
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