功能恢复力:全寄生植物的外来蛋白质中普遍存在活跃的氧化磷酸化系统

IF 5.4 Q1 PLANT SCIENCES
L.M. Gatica-Soria , M.V. Canal , M.E. Roulet , H. Sato , V. Gómez Villafañe , E. Welchen , M.V. Sanchez-Puerta
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引用次数: 0

摘要

有丝分裂核不相容是由于位于核区和线粒体区的共同进化基因之间的协调功能失调造成的。水平基因转移(HGT)涉及从不相干的物种获取基因,当外来基因产物与本地基因产物相互作用时,尤其是在多亚基复合体中,会引发有丝分裂核不相容。最近的研究结果表明,全寄生植物 Lophophytum 属(Balanophoraceae)线粒体基因组中的 HGT 非常猖獗。在 Lophophytum 中,一些参与氧化磷酸化(OXPHOS)系统的线粒体基因是从豆科宿主那里获得的,而核编码的 OXPHOS 亚基则不同,它们仍然是原生的。这种双重嵌合 OXPHOS 的独特结构将本地核编码亚基与外来和本地线粒体编码亚基结合在一起,从而引发了关于本地和外来蛋白质之间的相互作用对叶绿体线粒体呼吸活性的潜在影响的问题。我们检查了线粒体的超微结构,通过 Western 印迹评估了蛋白质的表达,并通过耗氧率和腺苷酸含量分析了这些全寄生植物的细胞呼吸。令人惊讶的是,我们的研究结果表明,尽管外来同源物在功能上取代了几种本地蛋白亚基,但在 Lophophytum 中并没有破坏 OXPHOS 机制或活性。此外,鉴于它们的寄生生活方式和完全丧失的光合作用,对 OXPHOS 系统也没有明显的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Functional resilience: An active oxidative phosphorylation system prevails amid foreign proteins in holoparasitic plants

Mitonuclear incompatibility results from a breakdown of the coordinated function between co-evolved genes located in nuclear and mitochondrial compartments. Horizontal Gene Transfer (HGT), involving the acquisition of genes from unrelated species, can trigger mitonuclear incompatibilities when foreign gene products interact with native ones, particularly in multisubunit complexes. Recent findings highlighted rampant HGT in the mitochondrial genomes of holoparasitic plants of the genus Lophophytum (Balanophoraceae). In Lophophytum, some mitochondrial genes involved in the Oxidative Phosphorylation (OXPHOS) system were acquired from their legume hosts, unlike the nuclear-encoded OXPHOS subunits, which remain native. This unique configuration of a doubly chimeric OXPHOS, combining native nuclear-encoded subunits with both foreign and native mitochondrial-encoded subunits, raises questions regarding the potential effects of the interactions between native and foreign proteins on mitochondrial respiration activity in Lophophytum. We examined the mitochondrial ultrastructure, evaluated protein expression via Western blots, and analyzed cellular respiration through oxygen consumption rates and adenylate content in these holoparasitic plants. Surprisingly, our results revealed no disruption of the OXPHOS machinery or activity in Lophophytum despite the functional replacement of several native protein subunits by foreign homologs. Furthermore, there was no apparent impact on the OXPHOS system given their parasitic lifestyle and complete loss of photosynthesis.

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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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