{"title":"不同浓度的紫杉醇对西门塔尔牛冷冻和解冻精液质量的影响","authors":"S Zolfaghari, E Ayen, A Khaki","doi":"10.22099/IJVR.2023.47455.6849","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Artificial insemination (AI) is one of the most important reproductive technologies used to modify animals genetically. Using this method, the genetic composition of the herd can be improved and selected by choosing bulls with excellent genetic characteristics. Taxifolin (TXF), a plant flavonoid, has shown an antioxidative effect.</p><p><strong>Aims: </strong>The current study aimed to evaluate the impact of TXF on the quality of frozen-thawed semen in Fleckvieh (Simmental) dual-purpose bulls.</p><p><strong>Methods: </strong>Freezable semen specimens were obtained by an artificial vagina. Ejaculates were equally divided into six parts for six experimental groups, including without adding TXF to diluent (C), adding 25 (T25), 50 (T50), 100 (T100), 200 (T200), and 400 (T400) μM TXF. Sperms were frozen in a one-step dilution method. Semen factors, including motility, viability, sperm membrane integrity, DNA damage, and oxidant and antioxidant enzyme activities, were examined after thawing.</p><p><strong>Results: </strong>Our findings revealed that all semen quality parameters, antioxidant enzyme activities, and free radical levels were superior in TXF-treated groups compared to the control group, and the differences were noticeably higher in the T100 group than the other groups.</p><p><strong>Conclusion: </strong>Adding 100 μM TXF to diluent could improve the quality of bull frozen semen.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"24 3","pages":"235-241"},"PeriodicalIF":0.8000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10804431/pdf/","citationCount":"0","resultStr":"{\"title\":\"The effect of different concentrations of taxifolin on the quality of frozen and thawed semen of Simmental cattle.\",\"authors\":\"S Zolfaghari, E Ayen, A Khaki\",\"doi\":\"10.22099/IJVR.2023.47455.6849\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Artificial insemination (AI) is one of the most important reproductive technologies used to modify animals genetically. Using this method, the genetic composition of the herd can be improved and selected by choosing bulls with excellent genetic characteristics. Taxifolin (TXF), a plant flavonoid, has shown an antioxidative effect.</p><p><strong>Aims: </strong>The current study aimed to evaluate the impact of TXF on the quality of frozen-thawed semen in Fleckvieh (Simmental) dual-purpose bulls.</p><p><strong>Methods: </strong>Freezable semen specimens were obtained by an artificial vagina. Ejaculates were equally divided into six parts for six experimental groups, including without adding TXF to diluent (C), adding 25 (T25), 50 (T50), 100 (T100), 200 (T200), and 400 (T400) μM TXF. Sperms were frozen in a one-step dilution method. Semen factors, including motility, viability, sperm membrane integrity, DNA damage, and oxidant and antioxidant enzyme activities, were examined after thawing.</p><p><strong>Results: </strong>Our findings revealed that all semen quality parameters, antioxidant enzyme activities, and free radical levels were superior in TXF-treated groups compared to the control group, and the differences were noticeably higher in the T100 group than the other groups.</p><p><strong>Conclusion: </strong>Adding 100 μM TXF to diluent could improve the quality of bull frozen semen.</p>\",\"PeriodicalId\":14629,\"journal\":{\"name\":\"Iranian journal of veterinary research\",\"volume\":\"24 3\",\"pages\":\"235-241\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10804431/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian journal of veterinary research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.22099/IJVR.2023.47455.6849\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.22099/IJVR.2023.47455.6849","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
The effect of different concentrations of taxifolin on the quality of frozen and thawed semen of Simmental cattle.
Background: Artificial insemination (AI) is one of the most important reproductive technologies used to modify animals genetically. Using this method, the genetic composition of the herd can be improved and selected by choosing bulls with excellent genetic characteristics. Taxifolin (TXF), a plant flavonoid, has shown an antioxidative effect.
Aims: The current study aimed to evaluate the impact of TXF on the quality of frozen-thawed semen in Fleckvieh (Simmental) dual-purpose bulls.
Methods: Freezable semen specimens were obtained by an artificial vagina. Ejaculates were equally divided into six parts for six experimental groups, including without adding TXF to diluent (C), adding 25 (T25), 50 (T50), 100 (T100), 200 (T200), and 400 (T400) μM TXF. Sperms were frozen in a one-step dilution method. Semen factors, including motility, viability, sperm membrane integrity, DNA damage, and oxidant and antioxidant enzyme activities, were examined after thawing.
Results: Our findings revealed that all semen quality parameters, antioxidant enzyme activities, and free radical levels were superior in TXF-treated groups compared to the control group, and the differences were noticeably higher in the T100 group than the other groups.
Conclusion: Adding 100 μM TXF to diluent could improve the quality of bull frozen semen.
期刊介绍：
The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.
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