在前肠分化过程中,GLI代码控制着HNF1A的水平。

Lucas Unger, Andreas F Mathisen, Simona Chera, Thomas Aga Legøy, Luiza Ghila
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引用次数: 0

摘要

人类诱导多能干细胞向胰岛内分泌细胞的分化是一个复杂的过程,涉及对关键发育途径的逐步调节,如在早期分化阶段对刺猬信号的抑制。在这种主动抑制的同时,胰岛内分泌细胞命运的关键转录因子(如 HNF1A)的表达模式也会发生特定的变化。在此,我们设计了一项试验性研究,旨在通过诱导 GLI 编码的变化,研究 HH 信号抑制与早期再生过程中 HNF1A 表达增加之间的潜在相互联系。这揭示了两者之间的联系,即 GLI3-R 介导的刺猬靶基因抑制显然是 HNF1A 有效表达所必需的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The GLI code controls HNF1A levels during foregut differentiation.

Differentiation of human induced pluripotent stem cells towards pancreatic islet endocrine cells is a complex process, involving the stepwise modulation of key developmental pathways, such as the Hedgehog signaling inhibition during early differentiation stages. In tandem with this active inhibition, key transcription factors for the islet endocrine cell fate, such as HNF1A, show specific changes in their expression patterns. Here we designed a pilot study aimed at investigating the potential interconnection between HH-signaling inhibition and the increase in the HNF1A expression during early regeneration, by inducing changes in the GLI code. This unveiled a link between the two, where GLI3-R mediated Hedgehog target genes inhibition is apparently required for HNF1A efficient expression.

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