{"title":"从牛乳腺炎牛奶样本中分离出的金黄色葡萄球菌的毒力基因和抗生素耐药性谱。","authors":"Z Hemati, L Abdolmohammadi Khiav, A Zahmatkesh","doi":"10.22099/IJVR.2023.45279.6652","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The increasing importance of antibiotic resistance shows the need for determining indices of the epidemiology of infection.</p><p><strong>Aims: </strong>This study aimed to determine the virulence genes and antibiotic resistance profiles of <i>Staphylococcus aureus</i> isolated from bovine mastitis cases.</p><p><strong>Methods: </strong>A total of 200 cattle were selected based on California Mastitis Test (CMT) results, and the samples were cultured in the laboratory. Grown colonies were examined by conventional phenotypic methods and confirmed using PCR amplification of <i>16S rRNA</i> gene. The prevalence of the virulence genes was also defined. The results of phenotypic and molecular tests were compared using SPSS software by McNemar test. Then, the confirmed isolates were tested for antibiotic susceptibility using the disc diffusion method.</p><p><strong>Results: </strong>Of the 200 positive CMT cattle, 24 animals were positive for <i>S. aureus</i> and confirmed using <i>16S rRNA</i> gene amplification. Statistical analysis showed that the phenotypic and genotypic tests of hemolysin genes were not significantly different (P>0.01). PCR analysis revealed the presence of <i>coa</i> and <i>clfa</i> genes in more than half of the cases. Overall, nine genetic profiles of virulence factors were found among <i>S. aureus</i> isolates. The highest and lowest resistance rates were against penicillin and gentamicin, respectively.</p><p><strong>Conclusion: </strong>Our findings showed a high rate of antibiotic resistance. So, accurate and fast diagnosis and antimicrobial susceptibility tests should be considered before prescribing the drugs.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"24 3","pages":"258-264"},"PeriodicalIF":0.8000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10804425/pdf/","citationCount":"0","resultStr":"{\"title\":\"Virulence genes and antibiotic resistance profiles of <i>Staphylococcus aureus</i> isolated from bovine mastitis milk samples.\",\"authors\":\"Z Hemati, L Abdolmohammadi Khiav, A Zahmatkesh\",\"doi\":\"10.22099/IJVR.2023.45279.6652\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The increasing importance of antibiotic resistance shows the need for determining indices of the epidemiology of infection.</p><p><strong>Aims: </strong>This study aimed to determine the virulence genes and antibiotic resistance profiles of <i>Staphylococcus aureus</i> isolated from bovine mastitis cases.</p><p><strong>Methods: </strong>A total of 200 cattle were selected based on California Mastitis Test (CMT) results, and the samples were cultured in the laboratory. Grown colonies were examined by conventional phenotypic methods and confirmed using PCR amplification of <i>16S rRNA</i> gene. The prevalence of the virulence genes was also defined. The results of phenotypic and molecular tests were compared using SPSS software by McNemar test. Then, the confirmed isolates were tested for antibiotic susceptibility using the disc diffusion method.</p><p><strong>Results: </strong>Of the 200 positive CMT cattle, 24 animals were positive for <i>S. aureus</i> and confirmed using <i>16S rRNA</i> gene amplification. Statistical analysis showed that the phenotypic and genotypic tests of hemolysin genes were not significantly different (P>0.01). PCR analysis revealed the presence of <i>coa</i> and <i>clfa</i> genes in more than half of the cases. Overall, nine genetic profiles of virulence factors were found among <i>S. aureus</i> isolates. The highest and lowest resistance rates were against penicillin and gentamicin, respectively.</p><p><strong>Conclusion: </strong>Our findings showed a high rate of antibiotic resistance. So, accurate and fast diagnosis and antimicrobial susceptibility tests should be considered before prescribing the drugs.</p>\",\"PeriodicalId\":14629,\"journal\":{\"name\":\"Iranian journal of veterinary research\",\"volume\":\"24 3\",\"pages\":\"258-264\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10804425/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian journal of veterinary research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.22099/IJVR.2023.45279.6652\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.22099/IJVR.2023.45279.6652","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Virulence genes and antibiotic resistance profiles of Staphylococcus aureus isolated from bovine mastitis milk samples.
Background: The increasing importance of antibiotic resistance shows the need for determining indices of the epidemiology of infection.
Aims: This study aimed to determine the virulence genes and antibiotic resistance profiles of Staphylococcus aureus isolated from bovine mastitis cases.
Methods: A total of 200 cattle were selected based on California Mastitis Test (CMT) results, and the samples were cultured in the laboratory. Grown colonies were examined by conventional phenotypic methods and confirmed using PCR amplification of 16S rRNA gene. The prevalence of the virulence genes was also defined. The results of phenotypic and molecular tests were compared using SPSS software by McNemar test. Then, the confirmed isolates were tested for antibiotic susceptibility using the disc diffusion method.
Results: Of the 200 positive CMT cattle, 24 animals were positive for S. aureus and confirmed using 16S rRNA gene amplification. Statistical analysis showed that the phenotypic and genotypic tests of hemolysin genes were not significantly different (P>0.01). PCR analysis revealed the presence of coa and clfa genes in more than half of the cases. Overall, nine genetic profiles of virulence factors were found among S. aureus isolates. The highest and lowest resistance rates were against penicillin and gentamicin, respectively.
Conclusion: Our findings showed a high rate of antibiotic resistance. So, accurate and fast diagnosis and antimicrobial susceptibility tests should be considered before prescribing the drugs.
期刊介绍：
The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.
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