{"title":"沙特阿拉伯一家医院临床分离的大肠埃希菌中质粒介导的喹诺酮耐药性和 16S rRNA 甲基化酶基因的流行情况。","authors":"Mutasim E Ibrahim","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The plasmid-mediated quinolone resistance (PMQR) and 16S ribosomal RNA (rRNA) methylase genes can lead to high-level bacterial resistance to various antibiotics, including quinolones and aminoglycosides. This study determined the prevalence of PMQR and 16S rRNA methylase determinants in clinical Escherichia coli. Non-duplicate E. coli resistant to quinolones (nalidixic acid, ciprofloxacin, or norfloxacin) and/or aminoglycosides (amikacin or gentamicin) were collected from patients at King Abdullah Hospital, Bisha, Saudi Arabia. A multiplex PCR was performed to identify the targeted genes. Out of 107 screened isolates, 44 (41%) were found to carry resistance genes, individually or in combination, including 41 PMQR and 14 16S rRNA methylase. The qnrS gene had the highest prevalence (23.4%) among PMQR, followed by aac(6')-Ib (16.8%). Of the 14 identified 16S rRNA methylase, 8 were rmtB and 6 were armA. Out of 44 positive isolates, 72.7% carried only one resistance gene, 25% had co-existing resistance genes, with qnrS and rmtB or aac(6)-Ib and armA being the most common pairs. A single isolate was carried three genes (qnrS, aac(6')-Ib and rmtB). As antibiotic resistance continues to become more prevalent, there is a need for extensive research to identify the genetic determinants of resistance and to develop new antibiotic therapies.</p>","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prevalence of plasmid-mediated quinolone resistance and 16S rRNA methylase genes among Escherichia coli clinical isolates in a hospital in Saudi Arabia.\",\"authors\":\"Mutasim E Ibrahim\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The plasmid-mediated quinolone resistance (PMQR) and 16S ribosomal RNA (rRNA) methylase genes can lead to high-level bacterial resistance to various antibiotics, including quinolones and aminoglycosides. This study determined the prevalence of PMQR and 16S rRNA methylase determinants in clinical Escherichia coli. Non-duplicate E. coli resistant to quinolones (nalidixic acid, ciprofloxacin, or norfloxacin) and/or aminoglycosides (amikacin or gentamicin) were collected from patients at King Abdullah Hospital, Bisha, Saudi Arabia. A multiplex PCR was performed to identify the targeted genes. Out of 107 screened isolates, 44 (41%) were found to carry resistance genes, individually or in combination, including 41 PMQR and 14 16S rRNA methylase. The qnrS gene had the highest prevalence (23.4%) among PMQR, followed by aac(6')-Ib (16.8%). Of the 14 identified 16S rRNA methylase, 8 were rmtB and 6 were armA. Out of 44 positive isolates, 72.7% carried only one resistance gene, 25% had co-existing resistance genes, with qnrS and rmtB or aac(6)-Ib and armA being the most common pairs. A single isolate was carried three genes (qnrS, aac(6')-Ib and rmtB). As antibiotic resistance continues to become more prevalent, there is a need for extensive research to identify the genetic determinants of resistance and to develop new antibiotic therapies.</p>\",\"PeriodicalId\":19971,\"journal\":{\"name\":\"Pakistan journal of pharmaceutical sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2023-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pakistan journal of pharmaceutical sciences\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pakistan journal of pharmaceutical sciences","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Prevalence of plasmid-mediated quinolone resistance and 16S rRNA methylase genes among Escherichia coli clinical isolates in a hospital in Saudi Arabia.
The plasmid-mediated quinolone resistance (PMQR) and 16S ribosomal RNA (rRNA) methylase genes can lead to high-level bacterial resistance to various antibiotics, including quinolones and aminoglycosides. This study determined the prevalence of PMQR and 16S rRNA methylase determinants in clinical Escherichia coli. Non-duplicate E. coli resistant to quinolones (nalidixic acid, ciprofloxacin, or norfloxacin) and/or aminoglycosides (amikacin or gentamicin) were collected from patients at King Abdullah Hospital, Bisha, Saudi Arabia. A multiplex PCR was performed to identify the targeted genes. Out of 107 screened isolates, 44 (41%) were found to carry resistance genes, individually or in combination, including 41 PMQR and 14 16S rRNA methylase. The qnrS gene had the highest prevalence (23.4%) among PMQR, followed by aac(6')-Ib (16.8%). Of the 14 identified 16S rRNA methylase, 8 were rmtB and 6 were armA. Out of 44 positive isolates, 72.7% carried only one resistance gene, 25% had co-existing resistance genes, with qnrS and rmtB or aac(6)-Ib and armA being the most common pairs. A single isolate was carried three genes (qnrS, aac(6')-Ib and rmtB). As antibiotic resistance continues to become more prevalent, there is a need for extensive research to identify the genetic determinants of resistance and to develop new antibiotic therapies.
期刊介绍:
Pakistan Journal of Pharmaceutical Sciences (PJPS) is a peer reviewed multi-disciplinary pharmaceutical sciences journal. The PJPS had its origin in 1988 from the Faculty of Pharmacy, University of Karachi as a biannual journal, frequency converted as quarterly in 2005, and now PJPS is being published as bi-monthly from January 2013.
PJPS covers Biological, Pharmaceutical and Medicinal Research (Drug Delivery, Pharmacy Management, Molecular Biology, Biochemical, Pharmacology, Pharmacokinetics, Phytochemical, Bio-analytical, Therapeutics, Biotechnology and research on nano particles.