对杰弗里蛛猴(Ateles geoffroyi)粪便中糖皮质激素代谢物的无创测量进行分析和生物学验证。

IF 2 3区 生物学 Q1 ZOOLOGY
Juliane Damm, Filippo Aureli, Ariadna Rangel-Negrín, Miriam Barradas-Moctezuma, Pedro A. D. Dias
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引用次数: 0

摘要

我们报告了一种商用皮质醇酶免疫分析法(EIA)的分析和生物学验证,该方法用于测量杰弗里蜘蛛猴(Ateles geoffroyi)粪便中的糖皮质激素(GC)。对每种样品基质和研究物种的内分泌学方法进行验证对于确定这些方法能产生可靠的结果至关重要。在 EIA 的分析验证中,我们评估了平行性、准确性和精确性。我们根据三种经过充分研究的 GC 模式进行了生物验证,预测结果如下:(1) 兽医干预后粪便 GC 代谢物 (fGCM) 浓度增加;(2) 清晨粪便 GC 代谢物浓度增加;(3) 妊娠期粪便 GC 代谢物浓度高于其他雌性生殖状态。针对第一个预测,我们在兽医干预前 2 天、兽医干预当天和兽医干预后 2 天对动物园饲养的两只雌性动物的粪便进行了采样。对于第二个预测,我们使用线性混合模型(LMM)分析了从 12 只野生雄性动物身上采集的 284 份粪便样本。对于第三个预测,我们使用线性混合模型分析了 8 只野生雌性动物的 269 份粪便样本。分析验证结果表明,EIA 在每次检测中都表现出平行性、准确性和精确性。不过,不同检测方法之间的精确度差异较大。生物学验证支持所有预测:(1)两只动物园饲养的雌性动物在兽医干预 2.5 小时和 11 小时后 fGCM 浓度显著增加;(2)样本采集时间对 fGCM 浓度有负面影响(即清晨浓度较高);(3)妊娠期雌性动物的 fGCM 浓度显著高于哺乳期雌性动物。因此,我们对商用 EIA 进行了分析验证,尽管检测之间存在差异,但我们仍能在人工饲养和野生环境中,以及在雄性和雌性动物体内发现三种与生物相关的 GC 信号。因此,我们相信这种方法可用于无创解决杰弗里蛛猴的行为内分泌学问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Analytical and biological validation of a noninvasive measurement of glucocorticoid metabolites in feces of Geoffroy's spider monkeys (Ateles geoffroyi)

Analytical and biological validation of a noninvasive measurement of glucocorticoid metabolites in feces of Geoffroy's spider monkeys (Ateles geoffroyi)

Analytical and biological validation of a noninvasive measurement of glucocorticoid metabolites in feces of Geoffroy's spider monkeys (Ateles geoffroyi)

We report on an analytical and biological validation of a commercial cortisol enzyme immunoassay (EIA) to measure glucocorticoids (GC) in feces of Geoffroy's spider monkeys (Ateles geoffroyi). Validation of endocrinological methods for each sample matrix and study species is crucial to establish that the methods produce reliable results. For the analytical validation of the EIA, we assessed parallelism, accuracy, and precision. We carried out a biological validation based on three well-studied GC patterns with the following predictions: (1) increased fecal GC metabolite (fGCM) concentrations after veterinary intervention; (2) increased fGCM concentrations during early morning hours; and (3) higher fGCM concentrations during gestation than in other female reproductive states. For the first prediction, we sampled feces of two zoo-housed females 2 days before, the day of, and 2 days after a veterinary intervention. For the second prediction, we analyzed 284 fecal samples collected from 12 wild males using a linear mixed model (LMM). For the third prediction, we analyzed 269 fecal samples of eight wild females using an LMM. Analytical validation revealed that the EIA showed parallelism, was accurate, and precise within each assay. However, there was elevated variation in between-assay precision. The biological validation supported all predictions: (1) the two zoo-housed females showed a substantial increase in fGCM concentrations 2.5 and 11 h after veterinary intervention; (2) there was a negative effect of sample collection time on fGCM concentrations (i.e., higher concentrations during early morning); (3) gestating females had significantly higher fGCM concentrations than lactating females. Thus, we analytically validated the commercial EIA and, despite between-assay variation, we were able to find three biologically relevant GC signals in captive and wild settings, and in males and females. We are therefore confident that the method can be used to noninvasively address behavioral endocrinology questions in Geoffroy's spider monkeys.

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来源期刊
CiteScore
4.50
自引率
8.30%
发文量
103
审稿时长
4-8 weeks
期刊介绍: The objective of the American Journal of Primatology is to provide a forum for the exchange of ideas and findings among primatologists and to convey our increasing understanding of this order of animals to specialists and interested readers alike. Primatology is an unusual science in that its practitioners work in a wide variety of departments and institutions, live in countries throughout the world, and carry out a vast range of research procedures. Whether we are anthropologists, psychologists, biologists, or medical researchers, whether we live in Japan, Kenya, Brazil, or the United States, whether we conduct naturalistic observations in the field or experiments in the lab, we are united in our goal of better understanding primates. Our studies of nonhuman primates are of interest to scientists in many other disciplines ranging from entomology to sociology.
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