放线菌天然产物生物合成基因簇的启动子工程：概念与应用。

IF 12.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

ACS Central Science Pub Date : 2024-04-24 DOI:10.1039/d3np00049d

Chang-Hun Ji , Hyun-Woo Je , Hiyoung Kim , Hahk-Soo Kang

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引用次数: 0

摘要

覆盖 2011 年至 2022 年天然产物在实验室培养或发酵条件下的低滴度一直是天然产物研究中的挑战性问题之一。许多天然产物生物合成基因簇（BGCs）在实验室培养条件下也处于转录沉默状态，这给鉴定其代谢产物的结构和活性带来了挑战。启动子工程为生物合成基因的转录激活或优化提供了工具，从而为这一问题提供了潜在的解决方案。在这篇综述中，我们总结了启动子工程方法在天然产品研究中的 10 年进展，重点是代谢天赋最高的放线菌属。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Promoter engineering of natural product biosynthetic gene clusters in actinomycetes: concepts and applications

查看原文本刊更多论文

Promoter engineering of natural product biosynthetic gene clusters in actinomycetes: concepts and applications

Covering 2011 to 2022

Low titers of natural products in laboratory culture or fermentation conditions have been one of the challenging issues in natural products research. Many natural product biosynthetic gene clusters (BGCs) are also transcriptionally silent in laboratory culture conditions, making it challenging to characterize the structures and activities of their metabolites. Promoter engineering offers a potential solution to this problem by providing tools for transcriptional activation or optimization of biosynthetic genes. In this review, we summarize the 10 years of progress in promoter engineering approaches in natural products research focusing on the most metabolically talented group of bacteria actinomycetes.

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来源期刊

ACS Central Science Chemical Engineering-General Chemical Engineering

CiteScore

25.50

自引率

0.50%

发文量

194

审稿时长

10 weeks

期刊介绍： ACS Central Science publishes significant primary reports on research in chemistry and allied fields where chemical approaches are pivotal. As the first fully open-access journal by the American Chemical Society, it covers compelling and important contributions to the broad chemistry and scientific community. "Central science," a term popularized nearly 40 years ago, emphasizes chemistry's central role in connecting physical and life sciences, and fundamental sciences with applied disciplines like medicine and engineering. The journal focuses on exceptional quality articles, addressing advances in fundamental chemistry and interdisciplinary research.