Da Hee Yang, Ha Young Lee, Woohyuk Choi, Chang-Lim Hyun, Ki Soo Kang
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Cytokines of sera such as tumor necrosis factor alpha (TNF-α) and CX3CL1 were analyzed using enzyme- linked immunosorbent assay (ELISA).</p><p><strong>Results: </strong>In the immunohistochemistry of gastric mucosa, the frequency of CD8<sup>+</sup> and T-bet<sup>+</sup> T cells cells was higher in the <i>H. pylori</i>-positive group than in the control group (26.9± 7.8% vs. 16.9±3.3%, <i>p</i><0.001; 5.0±2.5% vs. 2.2±0.7%, <i>p</i>=0.001). Between the control and <i>H. pylori</i>-positive groups, the frequency of IL-7Rα<sup>low</sup>CX3CR1<sup>+</sup> CD8<sup>+</sup> and T-bet<sup>+</sup> INF-γ<sup>+</sup> CD8<sup>+</sup> T cells were not significantly different between surface and intracellular staining, respectively (40.4±24.0% vs. 38.2±17.8%, <i>p</i>=0.914; 40.4±24.0% vs. 38.2±17.8%, <i>p</i>=0.914). In the ELISA, no significant differences in TNF-α and CX3CL1 concentrations were observed between the control and <i>H. pylori</i>-positive groups (34.3±12.1 pg/mL vs. 47.0±22.6 pg/mL, <i>p</i>=0.114/0.5± 0.1 pg/mL vs. 0.5±0.1 pg/mL, <i>p</i>=0.188).</p><p><strong>Conclusion: </strong>CD8<sup>+</sup> T and Th1 cells, which secrete IFN-γ, might play important roles in the mucosal immunity of the stomach in children with <i>H. pylori</i> infection.</p>","PeriodicalId":19989,"journal":{"name":"Pediatric Gastroenterology, Hepatology & Nutrition","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10796262/pdf/","citationCount":"0","resultStr":"{\"title\":\"Mucosal Immunity Related to CD8<sup>+</sup> T Lymphocytes in Children with <i>Helicobacter pylori</i> Gastritis.\",\"authors\":\"Da Hee Yang, Ha Young Lee, Woohyuk Choi, Chang-Lim Hyun, Ki Soo Kang\",\"doi\":\"10.5223/pghn.2024.27.1.26\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>We investigated the role of CD8<sup>+</sup>T cells as host immune factors in pediatric patients with <i>Helicobacter pylori</i> gastritis.</p><p><strong>Methods: </strong>Gastric mucosal tissue and blood samples were collected from 39 children, including 11 children with <i>H. pylori</i> infection and 28 children as controls. Anti-CD8 and anti-T-bet antibodies were used for immunohistochemistry of the gastric mucosa. For the cell surface and intracellular staining, peripheral blood mononuclear cells were stained with anti-IL7Rα, anti-CX3CR1, anti-CD8, anti-T-bet, and anti-IFN-γ antibodies. Cytokines of sera such as tumor necrosis factor alpha (TNF-α) and CX3CL1 were analyzed using enzyme- linked immunosorbent assay (ELISA).</p><p><strong>Results: </strong>In the immunohistochemistry of gastric mucosa, the frequency of CD8<sup>+</sup> and T-bet<sup>+</sup> T cells cells was higher in the <i>H. pylori</i>-positive group than in the control group (26.9± 7.8% vs. 16.9±3.3%, <i>p</i><0.001; 5.0±2.5% vs. 2.2±0.7%, <i>p</i>=0.001). Between the control and <i>H. pylori</i>-positive groups, the frequency of IL-7Rα<sup>low</sup>CX3CR1<sup>+</sup> CD8<sup>+</sup> and T-bet<sup>+</sup> INF-γ<sup>+</sup> CD8<sup>+</sup> T cells were not significantly different between surface and intracellular staining, respectively (40.4±24.0% vs. 38.2±17.8%, <i>p</i>=0.914; 40.4±24.0% vs. 38.2±17.8%, <i>p</i>=0.914). 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引用次数: 0
摘要
目的：我们研究了CD8+T细胞作为宿主免疫因子在幽门螺杆菌胃炎儿童患者中的作用：方法：收集 39 名儿童的胃黏膜组织和血液样本，包括 11 名幽门螺杆菌感染儿童和 28 名对照组儿童。使用抗 CD8 和抗 T-bet 抗体对胃黏膜进行免疫组化。在细胞表面和细胞内染色时，用抗IL7Rα、抗CX3CR1、抗CD8、抗T-bet和抗IFN-γ抗体对外周血单核细胞进行染色。使用酶联免疫吸附试验（ELISA）分析血清中的细胞因子，如肿瘤坏死因子α（TNF-α）和 CX3CL1：结果：在胃黏膜免疫组化中，幽门螺杆菌阳性组 CD8+ 和 T-bet+ T 细胞的频率高于对照组（26.9± 7.8% vs. 16.9±3.3%，pp=0.001）。在对照组和幽门螺杆菌阳性组之间，IL-7Rα-lowCX3CR1+ CD8+和T-bet+ INF-γ+ CD8+T细胞的频率在表面染色和细胞内染色之间分别无显著差异（40.4±24.0% vs. 38.2±17.8%，p=0.914；40.4±24.0% vs. 38.2±17.8%，p=0.914）。在酶联免疫吸附试验中，对照组和幽门螺杆菌阳性组的TNF-α和CX3CL1浓度无明显差异（34.3±12.1 pg/mL vs. 47.0±22.6 pg/mL，p=0.114/0.5±0.1 pg/mL vs. 0.5±0.1 pg/mL，p=0.188）：结论：分泌 IFN-γ 的 CD8+ T 细胞和 Th1 细胞可能在幽门螺杆菌感染儿童的胃黏膜免疫中发挥重要作用。
Mucosal Immunity Related to CD8+ T Lymphocytes in Children with Helicobacter pylori Gastritis.
Purpose: We investigated the role of CD8+T cells as host immune factors in pediatric patients with Helicobacter pylori gastritis.
Methods: Gastric mucosal tissue and blood samples were collected from 39 children, including 11 children with H. pylori infection and 28 children as controls. Anti-CD8 and anti-T-bet antibodies were used for immunohistochemistry of the gastric mucosa. For the cell surface and intracellular staining, peripheral blood mononuclear cells were stained with anti-IL7Rα, anti-CX3CR1, anti-CD8, anti-T-bet, and anti-IFN-γ antibodies. Cytokines of sera such as tumor necrosis factor alpha (TNF-α) and CX3CL1 were analyzed using enzyme- linked immunosorbent assay (ELISA).
Results: In the immunohistochemistry of gastric mucosa, the frequency of CD8+ and T-bet+ T cells cells was higher in the H. pylori-positive group than in the control group (26.9± 7.8% vs. 16.9±3.3%, p<0.001; 5.0±2.5% vs. 2.2±0.7%, p=0.001). Between the control and H. pylori-positive groups, the frequency of IL-7RαlowCX3CR1+ CD8+ and T-bet+ INF-γ+ CD8+ T cells were not significantly different between surface and intracellular staining, respectively (40.4±24.0% vs. 38.2±17.8%, p=0.914; 40.4±24.0% vs. 38.2±17.8%, p=0.914). In the ELISA, no significant differences in TNF-α and CX3CL1 concentrations were observed between the control and H. pylori-positive groups (34.3±12.1 pg/mL vs. 47.0±22.6 pg/mL, p=0.114/0.5± 0.1 pg/mL vs. 0.5±0.1 pg/mL, p=0.188).
Conclusion: CD8+ T and Th1 cells, which secrete IFN-γ, might play important roles in the mucosal immunity of the stomach in children with H. pylori infection.
期刊介绍：
Pediatric Gastroenterology, Hepatology and Nutrition (Pediatr Gastroenterol Hepatol Nutr), an official journal of The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition, is issued bimonthly and published in English. The aim of Pediatr Gastroenterol Hepatol Nutr is to advance scientific knowledge and promote child healthcare by publishing high-quality empirical and theoretical studies and providing a recently updated knowledge to those practitioners and scholars in the field of pediatric gastroenterology, hepatology and nutrition. Pediatr Gastroenterol Hepatol Nutr publishes review articles, original articles, and case reports. All of the submitted papers are peer-reviewed. The journal covers basic and clinical researches on molecular and cellular biology, pathophysiology, epidemiology, diagnosis, and treatment of all aspects of pediatric gastrointestinal diseases and nutritional health problems.
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