蛋白负载型聚氯乙烯微胶囊的微流体模板化和免引发剂光交联技术

IF 2.7 4区 材料科学 Q3 CHEMISTRY, PHYSICAL
Florian Störmann, Toralf Roch, Andreas Lendlein, Christian Wischke
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引用次数: 0

摘要

聚合物网络材料是热塑性聚合物的有趣替代品。本文研究了聚合物胶囊的制备,这种胶囊由聚(ε-己内酯)(PCL)网络制成,由交联的 PCL 外壳和适合封装有效载荷的内核组成。在微流体模板技术的帮助下,通过甲基丙烯酸酯端基的无光引发剂紫外光诱导自由基聚合,从 4 臂星形 12 kDa PCL 前体中形成了尺寸分布窄(176 ± 5 µm)、外壳薄(≈7.5 µm)的 PCL 网络胶囊。FITC-BSA 被封装为模型蛋白质。胶囊的理化特性表明,甲基丙烯酸酯端基部分交联成了网点。显微镜检查发现了部分塌陷的胶囊,我们将结合网络稳定性和去除溶剂时胶囊界面产生的机械应力对其进行讨论。颗粒与人类胚胎肾(HEK)细胞的培养显示出良好的细胞兼容性,这表明它们在生物科学及其他领域具有潜在用途。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Microfluidic Templating and Initiator-Free Photocrosslinking of Protein-Loaded PCL Microcapsules

Microfluidic Templating and Initiator-Free Photocrosslinking of Protein-Loaded PCL Microcapsules
Polymer network materials are interesting alternatives to thermoplastic polymers. Here, the preparation of polymer capsules is investigated, which are made from poly(ε-caprolactone) (PCL) networks and are compartmentalized in a crosslinked PCL shell and a core that is suitable to enclose payloads of interest. Aided by microfluidic templating, PCL network capsules with a narrow size distribution (176 ± 5 µm) and thin shells (≈7.5 µm) are formed from 4-arm star-shaped 12 kDa PCL precursors by photoinitiator-free UV light-induced radical polymerization of methacrylate end-groups. FITC-BSA is encapsulated as a model protein. The physicochemical characterization of the capsules indicated a partial crosslinking of methacrylate endgroups into netpoints. Microscopy revealed a fraction of collapsed capsules that are discussed in the context of network stability and mechanical stress created at the capsule interfaces during solvent removal. The incubation of particles with human embryonic kidney (HEK) cells showed good cell compatibility, suggesting their potential use in biosciences and beyond.
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来源期刊
Particle & Particle Systems Characterization
Particle & Particle Systems Characterization 工程技术-材料科学:表征与测试
CiteScore
5.50
自引率
0.00%
发文量
114
审稿时长
3.0 months
期刊介绍: Particle & Particle Systems Characterization is an international, peer-reviewed, interdisciplinary journal focusing on all aspects of particle research. The journal joined the Advanced Materials family of journals in 2013. Particle has an impact factor of 4.194 (2018 Journal Impact Factor, Journal Citation Reports (Clarivate Analytics, 2019)). Topics covered include the synthesis, characterization, and application of particles in a variety of systems and devices. Particle covers nanotubes, fullerenes, micelles and alloy clusters, organic and inorganic materials, polymers, quantum dots, 2D materials, proteins, and other molecular biological systems. Particle Systems include those in biomedicine, catalysis, energy-storage materials, environmental science, micro/nano-electromechanical systems, micro/nano-fluidics, molecular electronics, photonics, sensing, and others. Characterization methods include microscopy, spectroscopy, electrochemical, diffraction, magnetic, and scattering techniques.
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