纳米级硫改变番茄根圈的细菌和真核生物群落及其与真菌病原体的相互作用

IF 4.7 3区 环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES
Blaire Steven , M. Amine Hassani , Jacquelyn C. LaReau , Yi Wang , Jason C. White
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引用次数: 0

摘要

当植物遇到病原体时,硫的纳米制剂已证明具有促进植物生长和降低病害发生率的潜力。然而,纳米级硫磺对与植物根部密切接触的微生物群落(即根瘤菌圈)的影响尚不十分明确。在本研究中,我们调查了三种硫磺配方(散装硫磺、无涂层(原始)硫磺纳米颗粒和硬脂酸涂层硫磺纳米颗粒)对番茄植株根瘤菌层的影响。番茄植株还受到了致病真菌 Fusarium oxysporum f. sp. Lycopersici 的挑战。我们利用细菌 16S rRNA 基因测序以及最近自行设计的肽核酸夹来促进微真核细胞序列的恢复,对根圈微生物种群进行了全面调查。我们发现,对根圈微生物群组成影响最大的是真菌病原体的存在。不过,硫添加剂也会导致根瘤菌群的状态发生变化,例如,对植物有益的硫氧化细菌 Thiobacillus 的相对丰度增加。值得注意的是,在研究根圈群落对不同硫添加剂的反应时,真菌病原体与硫处理之间存在强烈的相互作用。这导致不同的细菌和真核生物类群与不同形式的硫有关,而这取决于病原体的存在。这些数据表明,与散装硫磺相比,纳米硫磺配方在根瘤菌群落中产生了独特的变化,尤其是在与植物病原体结合的情况下。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nanoscale sulfur alters the bacterial and eukaryotic communities of the tomato rhizosphere and their interactions with a fungal pathogen

Nanoformulations of sulfur have demonstrated the potential to enhance plant growth and reduce disease incidence when plants are confronted with pathogens. However, the impact of nanoscale sulfur on microbial communities in close contact with the plant root, known as the rhizosphere, remain poorly characterized. In this study, we investigate the impact of three formulations of sulfur; bulk sulfur, uncoated (pristine) sulfur nanoparticles, and stearic acid coated sulfur nanoparticles, on the rhizosphere of tomato plants. Tomato plants were additionally challenged by the pathogenic fungus Fusarium oxysporum f. sp. Lycopersici. Employing bacterial 16S rRNA gene sequencing, along with recently in-house designed peptide nucleic acid clamps to facilitate the recovery of microeukaryote sequences, we performed a comprehensive survey of rhizosphere microbial populations. We found the largest influence on the composition of the rhizosphere microbiome was the presence of the fungal pathogen. However, sulfur amendments also drove state changes in the rhizosphere populations; for example, enriching the relative abundance of the plant-beneficial sulfur-oxidizing bacterium Thiobacillus. Notably, when investigating the response of the rhizosphere community to the different sulfur amendments, there was a strong interaction between the fungal pathogen and sulfur treatments. This resulted in different bacterial and eukaryotic taxa being enriched in association with the different forms of sulfur, which was dependent on the presence of the pathogen. These data point to nano formulations of sulfur exerting unique shifts in the rhizosphere community compared to bulk sulfur, particularly in association with a plant pathogen, and have implications for the sustainable use of nanoscale strategies in sustainable agriculture.

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来源期刊
NanoImpact
NanoImpact Social Sciences-Safety Research
CiteScore
11.00
自引率
6.10%
发文量
69
审稿时长
23 days
期刊介绍: NanoImpact is a multidisciplinary journal that focuses on nanosafety research and areas related to the impacts of manufactured nanomaterials on human and environmental systems and the behavior of nanomaterials in these systems.
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