海产壳寡糖对 AFB1 大鼠急性肝损伤的干预机制

IF 4.3 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Lin Chen, Jiahui Yan, Huijun Shi, Zhaohuan Zhang, YueLiang Zhao, Yong Zhao, Yuan Wang, Jie Ou
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引用次数: 0

摘要

黄曲霉毒素 B1(AFB1)具有极强的肝毒性,是肝癌的致病因子,可引起急性或慢性肝损伤症状。从虾壳和蟹壳中提取的壳聚糖降解后得到的壳寡糖(COS)是一种天然抗氧化物质,其抗肿瘤特性已被广泛研究,但关于预防 AFB1 引起的急性肝损伤的研究较少。本研究将大鼠急性暴露于 1 毫克/千克体重的 AFB1,同时灌胃不同剂量的 COS,持续 8 天。结果表明,COS 可减轻大鼠肝组织病理变化,降低血清生化指标(谷丙转氨酶、谷草转氨酶、谷草转氨酶和 TBIL)。它能明显抑制 MDA 含量,促进 SOD 和 GSH-Px 活性的产生。此外,它还能改善肝细胞凋亡。此外,AFB1-vs-HCOS 差异基因富集了 622 个 GO 条目,其中 380 个为生物过程,170 个为分子功能,72 个为细胞成分。用 KEGG 富集方法分析的差异表达基因(DEGs)更多地富集在代谢、PPAR 信号通路和过氧化物酶体等通路中。Q-PCR技术证实,DEGs中的Lama5、Egr1、Cyp2b1和Gadd45g与氧化应激损伤和细胞凋亡有关。总之,COS干预可降低AFB1对肝脏基因的影响,从而减少肝脏基因功能的变化。 图文摘要
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Intervention mechanism of marine-based chito-oligosaccharide on acute liver injury induced by AFB1 in rats

Intervention mechanism of marine-based chito-oligosaccharide on acute liver injury induced by AFB1 in rats

Aflatoxin B1 (AFB1) is extremely hepatotoxic, a causative agent of liver cancer, and can cause symptoms of acute or chronic liver damage. Chito-oligosaccharides (COS), obtained from the degradation of chitosan derived from shrimp and crab shells, is a natural antioxidant substance and its antitumor properties have been widely studied, but less research has been done on the prevention of AFB1-induced acute liver injury. In this study, rats were acutely exposed to 1 mg/kg BW AFB1 and simultaneously gavaged with different doses of COS for 8 days. The results showed that COS attenuated the hepatic histopathological changes and reduced serum biochemical indices (ALT, AST, ALP, and TBIL) in rats. It significantly inhibited MDA content and promoted SOD and GSH-Px activity production. Moreover, it also improved hepatocyte apoptosis. Furthermore, AFB1-vs-HCOS differential genes were enriched with 622 GO entries, and 380 were Biological Processes, 170 were Molecular Functions, 72 were Cellular Components. Differentially expressed genes (DEGs) analyzed by KEGG enrichment were more enriched in pathways, such as metabolism, PPAR signaling pathway, and peroxisome. Q-PCR technique verified that Lama5, Egr1, Cyp2b1, and Gadd45g in DEGs were associated with oxidative stress damage and apoptosis. In conclusion, COS intervention reduces the effect of AFB1 on hepatic genes and thus reduces the changes in hepatic gene function.

Graphical abstract

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来源期刊
Bioresources and Bioprocessing
Bioresources and Bioprocessing BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
8.70%
发文量
118
审稿时长
13 weeks
期刊介绍: Bioresources and Bioprocessing (BIOB) is a peer-reviewed open access journal published under the brand SpringerOpen. BIOB aims at providing an international academic platform for exchanging views on and promoting research to support bioresource development, processing and utilization in a sustainable manner. As an application-oriented research journal, BIOB covers not only the application and management of bioresource technology but also the design and development of bioprocesses that will lead to new and sustainable production processes. BIOB publishes original and review articles on most topics relating to bioresource and bioprocess engineering, including: -Biochemical and microbiological engineering -Biocatalysis and biotransformation -Biosynthesis and metabolic engineering -Bioprocess and biosystems engineering -Bioenergy and biorefinery -Cell culture and biomedical engineering -Food, agricultural and marine biotechnology -Bioseparation and biopurification engineering -Bioremediation and environmental biotechnology
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