{"title":"鸡白细胞介素-5在脾淋巴细胞中表达并影响抗原特异性抗体的产生","authors":"Tenkai Watanabe, Takumi Terada, Ryo Ezaki, Mei Matsuzaki, Syuichi Furusawa, Hiroyuki Horiuchi","doi":"10.2141/jpsa.2024002","DOIUrl":null,"url":null,"abstract":"<p><p>Vaccination is important for reducing disease incidence in the poultry industry. To enhance immunity and vaccine efficacy, chicken cytokines associated with antibody production must be identified. In this study, we focused on interleukin-5 (IL-5), involved in antibody production in mice, measuring its expression and effects on antibody production. Concanavalin A-stimulated splenocytes were used for RT-PCR to clone <i>IL5</i> cDNAs. Recombinant IL-5 was prepared from the clone and administered to chickens with antigen via the ocular-topical route twice every alternate week. IL-5 enhanced antigen-specific IgY and inhibited antigen-specific serum IgA production in serum. Our findings suggest that IL-5 plays an important role in chicken antibody production, with possible unique functions.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":"61 ","pages":"2024002"},"PeriodicalIF":1.8000,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10789502/pdf/","citationCount":"0","resultStr":"{\"title\":\"Chicken Interleukin-5 is Expressed in Splenic Lymphocytes and Affects Antigen-Specific Antibody Production.\",\"authors\":\"Tenkai Watanabe, Takumi Terada, Ryo Ezaki, Mei Matsuzaki, Syuichi Furusawa, Hiroyuki Horiuchi\",\"doi\":\"10.2141/jpsa.2024002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Vaccination is important for reducing disease incidence in the poultry industry. To enhance immunity and vaccine efficacy, chicken cytokines associated with antibody production must be identified. In this study, we focused on interleukin-5 (IL-5), involved in antibody production in mice, measuring its expression and effects on antibody production. Concanavalin A-stimulated splenocytes were used for RT-PCR to clone <i>IL5</i> cDNAs. Recombinant IL-5 was prepared from the clone and administered to chickens with antigen via the ocular-topical route twice every alternate week. IL-5 enhanced antigen-specific IgY and inhibited antigen-specific serum IgA production in serum. Our findings suggest that IL-5 plays an important role in chicken antibody production, with possible unique functions.</p>\",\"PeriodicalId\":16883,\"journal\":{\"name\":\"Journal of Poultry Science\",\"volume\":\"61 \",\"pages\":\"2024002\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-01-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10789502/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Poultry Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2141/jpsa.2024002\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2141/jpsa.2024002","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
摘要
疫苗接种对于降低家禽业的疾病发病率非常重要。为了提高免疫力和疫苗效果，必须确定与抗体产生相关的鸡细胞因子。在本研究中，我们重点研究了参与小鼠抗体产生的白细胞介素-5（IL-5），测量了它的表达及其对抗体产生的影响。用康卡伐林 A 刺激的脾细胞进行 RT-PCR 克隆 IL5 cDNA。从克隆中制备重组IL-5，并通过眼部局部途径给鸡注射抗原，每隔一周两次。IL-5可增强抗原特异性IgY，抑制血清中抗原特异性血清IgA的产生。我们的研究结果表明，IL-5 在鸡抗体产生过程中发挥着重要作用，并可能具有独特的功能。
Chicken Interleukin-5 is Expressed in Splenic Lymphocytes and Affects Antigen-Specific Antibody Production.
Vaccination is important for reducing disease incidence in the poultry industry. To enhance immunity and vaccine efficacy, chicken cytokines associated with antibody production must be identified. In this study, we focused on interleukin-5 (IL-5), involved in antibody production in mice, measuring its expression and effects on antibody production. Concanavalin A-stimulated splenocytes were used for RT-PCR to clone IL5 cDNAs. Recombinant IL-5 was prepared from the clone and administered to chickens with antigen via the ocular-topical route twice every alternate week. IL-5 enhanced antigen-specific IgY and inhibited antigen-specific serum IgA production in serum. Our findings suggest that IL-5 plays an important role in chicken antibody production, with possible unique functions.
期刊介绍：
The Journal of Poultry Science will publish original reports and reviews which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: breeding and genetics, nutrition and feeds, physiology, reproduction, immunology, behavior, environmental science, management and housing welfare, processing and products, and health in poultry. Submission of original articles to the Journal is open to all poultry researchers. The review articles are invited papers written by international outstanding researchers. Articles will be published in English, American style.
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