{"title":"基于形态学和分子数据的中国块菌属物种多样性、系统发育、特有性和地理分布。","authors":"L Fan, T Li, Y Y Xu, X Y Yan","doi":"10.3767/persoonia.2023.48.05","DOIUrl":null,"url":null,"abstract":"<p><p>The genus <i>Tuber</i> (<i>Tuberaceae</i>, <i>Pezizales</i>) is an important fungal group of <i>Ascomycota</i> both economically and ecologically. However, the species diversity, phylogenetic relationships, and geographic distribution of <i>Tuber</i> species in China remains poorly understood, primarily because descriptions of many new species relied heavily on morphological features with molecular data either not sought or ignored. The misapplication of European and North American names further added to confusion regarding the taxonomy of Chinese <i>Tuber</i> species. In this study, we examined more than 1 000 specimens from China, and performed a comprehensive phylogenetic analysis for Chinese <i>Tuber</i> species using ITS sequences and multilocus sequence data. To infer the phylogeny of Chinese <i>Tuber</i> spp., 11 molecular datasets were assembled, including a concatenated internal transcribed spacers of the nuc rDNA (ITS), nuc rDNA 28S subunit (LSU), translation elongation factor 1-alpha (<i>tef1</i>-α), and RNA polymerase II subunit (<i>rpb2</i>) dataset as well as 10 ITS datasets (totally including 1 435 sequences from 828 collections with 597 newly generated sequences, and 168 sequences from the types of 63 species). Our phylogenetic tree based on a concatenated multilocus dataset revealed that all Chinese Tuber species nested in nine phylogenetic clades (phylogroups), including <i>Aestivum</i>, <i>Excavatum</i>, <i>Latisporum</i>, <i>Macrosporum</i>, <i>Maculatum</i>, <i>Melanosporum</i>, <i>Puberulum</i>, <i>Rufum</i> and <i>Turmericum</i>. Of these, five phylogroups (<i>Macrosporum</i>, <i>Maculatum</i>, <i>Melanosporum</i>, <i>Puberulum</i> and <i>Rufum</i>) are shared across the continents of Asia, Europe and North America; two phylogroups (<i>Aestivum</i> and <i>Excavatum</i>) are shared by Europe and Asia; and the phylogroups Turmericum and Latisporum are endemic only to Asia. Phylogenetic trees based on 10 ITS datasets confirmed the presence of at least 82 phylogenetic species in China. Of these, 53 are identified as known species, including three new records for China, and 25 species are identified as new to science. Of the new species, nine are described and illustrated in this paper, and the others remain unnamed due to the paucity or absence of ascomatal materials. Accordingly, the confirmed, excluded and doubtful Tuber species in China are discussed. Tuber species showed high endemism. Of the 82 phylogenetic species found in China, 68 species occur only in China, six species are also found in other regions in Asia, and only eight species (<i>T. anniae</i>, <i>T. excelsum-reticulatum</i>, <i>T. formosanum</i>, <i>T. maculatum</i>, <i>T. wenchuanense</i>, <i>Tuber</i> sp. CHN-3, <i>Tuber</i> sp. CHN-10 and <i>Tuber</i> sp. CHN-11) are shared with other continents. Most Tuber species have a small and limited distribution in China, but a few, such as <i>T. formosanum</i> and <i>T. parvomurphium</i>, are widely distributed across China. Some phylogenetically closely related species, such as <i>T. liaotongense</i> and <i>T. subglobosum</i>, as well as <i>T. xuanhuaense</i> and <i>T. lijiangense</i>, show a pattern of allopatric distribution.</p>","PeriodicalId":20014,"journal":{"name":"Persoonia","volume":null,"pages":null},"PeriodicalIF":9.5000,"publicationDate":"2022-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10792285/pdf/","citationCount":"0","resultStr":"{\"title\":\"Species diversity, phylogeny, endemism and geography of the truffle genus <i>Tuber</i> in China based on morphological and molecular data.\",\"authors\":\"L Fan, T Li, Y Y Xu, X Y Yan\",\"doi\":\"10.3767/persoonia.2023.48.05\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The genus <i>Tuber</i> (<i>Tuberaceae</i>, <i>Pezizales</i>) is an important fungal group of <i>Ascomycota</i> both economically and ecologically. However, the species diversity, phylogenetic relationships, and geographic distribution of <i>Tuber</i> species in China remains poorly understood, primarily because descriptions of many new species relied heavily on morphological features with molecular data either not sought or ignored. The misapplication of European and North American names further added to confusion regarding the taxonomy of Chinese <i>Tuber</i> species. In this study, we examined more than 1 000 specimens from China, and performed a comprehensive phylogenetic analysis for Chinese <i>Tuber</i> species using ITS sequences and multilocus sequence data. To infer the phylogeny of Chinese <i>Tuber</i> spp., 11 molecular datasets were assembled, including a concatenated internal transcribed spacers of the nuc rDNA (ITS), nuc rDNA 28S subunit (LSU), translation elongation factor 1-alpha (<i>tef1</i>-α), and RNA polymerase II subunit (<i>rpb2</i>) dataset as well as 10 ITS datasets (totally including 1 435 sequences from 828 collections with 597 newly generated sequences, and 168 sequences from the types of 63 species). Our phylogenetic tree based on a concatenated multilocus dataset revealed that all Chinese Tuber species nested in nine phylogenetic clades (phylogroups), including <i>Aestivum</i>, <i>Excavatum</i>, <i>Latisporum</i>, <i>Macrosporum</i>, <i>Maculatum</i>, <i>Melanosporum</i>, <i>Puberulum</i>, <i>Rufum</i> and <i>Turmericum</i>. Of these, five phylogroups (<i>Macrosporum</i>, <i>Maculatum</i>, <i>Melanosporum</i>, <i>Puberulum</i> and <i>Rufum</i>) are shared across the continents of Asia, Europe and North America; two phylogroups (<i>Aestivum</i> and <i>Excavatum</i>) are shared by Europe and Asia; and the phylogroups Turmericum and Latisporum are endemic only to Asia. Phylogenetic trees based on 10 ITS datasets confirmed the presence of at least 82 phylogenetic species in China. Of these, 53 are identified as known species, including three new records for China, and 25 species are identified as new to science. Of the new species, nine are described and illustrated in this paper, and the others remain unnamed due to the paucity or absence of ascomatal materials. Accordingly, the confirmed, excluded and doubtful Tuber species in China are discussed. Tuber species showed high endemism. Of the 82 phylogenetic species found in China, 68 species occur only in China, six species are also found in other regions in Asia, and only eight species (<i>T. anniae</i>, <i>T. excelsum-reticulatum</i>, <i>T. formosanum</i>, <i>T. maculatum</i>, <i>T. wenchuanense</i>, <i>Tuber</i> sp. CHN-3, <i>Tuber</i> sp. CHN-10 and <i>Tuber</i> sp. CHN-11) are shared with other continents. Most Tuber species have a small and limited distribution in China, but a few, such as <i>T. formosanum</i> and <i>T. parvomurphium</i>, are widely distributed across China. Some phylogenetically closely related species, such as <i>T. liaotongense</i> and <i>T. subglobosum</i>, as well as <i>T. xuanhuaense</i> and <i>T. lijiangense</i>, show a pattern of allopatric distribution.</p>\",\"PeriodicalId\":20014,\"journal\":{\"name\":\"Persoonia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":9.5000,\"publicationDate\":\"2022-07-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10792285/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Persoonia\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3767/persoonia.2023.48.05\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/5/27 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Persoonia","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3767/persoonia.2023.48.05","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/5/27 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"MYCOLOGY","Score":null,"Total":0}
Species diversity, phylogeny, endemism and geography of the truffle genus Tuber in China based on morphological and molecular data.
The genus Tuber (Tuberaceae, Pezizales) is an important fungal group of Ascomycota both economically and ecologically. However, the species diversity, phylogenetic relationships, and geographic distribution of Tuber species in China remains poorly understood, primarily because descriptions of many new species relied heavily on morphological features with molecular data either not sought or ignored. The misapplication of European and North American names further added to confusion regarding the taxonomy of Chinese Tuber species. In this study, we examined more than 1 000 specimens from China, and performed a comprehensive phylogenetic analysis for Chinese Tuber species using ITS sequences and multilocus sequence data. To infer the phylogeny of Chinese Tuber spp., 11 molecular datasets were assembled, including a concatenated internal transcribed spacers of the nuc rDNA (ITS), nuc rDNA 28S subunit (LSU), translation elongation factor 1-alpha (tef1-α), and RNA polymerase II subunit (rpb2) dataset as well as 10 ITS datasets (totally including 1 435 sequences from 828 collections with 597 newly generated sequences, and 168 sequences from the types of 63 species). Our phylogenetic tree based on a concatenated multilocus dataset revealed that all Chinese Tuber species nested in nine phylogenetic clades (phylogroups), including Aestivum, Excavatum, Latisporum, Macrosporum, Maculatum, Melanosporum, Puberulum, Rufum and Turmericum. Of these, five phylogroups (Macrosporum, Maculatum, Melanosporum, Puberulum and Rufum) are shared across the continents of Asia, Europe and North America; two phylogroups (Aestivum and Excavatum) are shared by Europe and Asia; and the phylogroups Turmericum and Latisporum are endemic only to Asia. Phylogenetic trees based on 10 ITS datasets confirmed the presence of at least 82 phylogenetic species in China. Of these, 53 are identified as known species, including three new records for China, and 25 species are identified as new to science. Of the new species, nine are described and illustrated in this paper, and the others remain unnamed due to the paucity or absence of ascomatal materials. Accordingly, the confirmed, excluded and doubtful Tuber species in China are discussed. Tuber species showed high endemism. Of the 82 phylogenetic species found in China, 68 species occur only in China, six species are also found in other regions in Asia, and only eight species (T. anniae, T. excelsum-reticulatum, T. formosanum, T. maculatum, T. wenchuanense, Tuber sp. CHN-3, Tuber sp. CHN-10 and Tuber sp. CHN-11) are shared with other continents. Most Tuber species have a small and limited distribution in China, but a few, such as T. formosanum and T. parvomurphium, are widely distributed across China. Some phylogenetically closely related species, such as T. liaotongense and T. subglobosum, as well as T. xuanhuaense and T. lijiangense, show a pattern of allopatric distribution.
期刊介绍:
Persoonia aspires to publish papers focusing on the molecular systematics and evolution of fungi. Additionally, it seeks to advance fungal taxonomy by employing a polythetic approach to elucidate the genuine phylogeny and relationships within the kingdom Fungi. The journal is dedicated to disseminating high-quality papers that unravel both known and novel fungal taxa at the DNA level. Moreover, it endeavors to provide fresh insights into evolutionary processes and relationships. The scope of papers considered encompasses research articles, along with topical and book reviews.