富血小板血浆和他达拉非对睾丸扭转/脱落大鼠睾丸缺血/再灌注损伤的保护作用的启示

IF 2.5 Q3 CELL BIOLOGY
Dalia M Abdel Ghaffar, Zienab Helmy Eldken, Mohammed S Sultan, Rania M Khalil, Noha Hamma Sakr, Hanan Eissa, Sally M Safwat
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引用次数: 0

摘要

背景/目的:缺血性再灌注(I-R)损伤在很大程度上受睾丸扭转/离体过程(TDP)的影响。在这种情况下,富血小板血浆(PRP)的抗炎特性结合他达拉非(Td)可显著促进 I-R 损伤模型的组织愈合:方法:将大鼠分为五组:对照组、未接受任何治疗的 I-R 组、接受单剂量 Td(0.25 毫克/千克,静脉注射)的 I-R 组、接受单剂量 PRP(80 升,睾丸内注射)的 I-R 组以及同时接受 Td 和 PRP 的 I-R 组。对精子的形态、活力和组织学进行了评估。测量了 TNF-、BAX、抗氧化状态和睾酮的水平。此外,还检测了 E 选择素的表达:结果:PRP 降低了氧化应激、炎症和细胞凋亡,同时还提高了睾酮水平,从而减轻了 I-R 损伤。此外,PRP 还能减少 E 选择素的表达,从而改变控制内皮功能的途径。与此同时,Td 也部分证明了其对睾丸的保护作用:结论:PRP 的抗炎、抗氧化和抗细胞凋亡潜能已得到证实,这使其成为治疗他达拉非引起的睾丸损伤的天然疗法。研究首次证明,PRP疗法能恢复血管内皮的功能,特别是控制E-选择素的表达。将Td和PRP疗法结合起来可能是改善对PDE5抑制剂反应的一种有前途的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Insights on Protective Effect of Platelet Rich Plasma and Tadalafil on Testicular Ischemia/Reperfusion Injury in Rats Exposed to Testicular Torsion/Detorsion.

Background/aims: Ischemic reperfusion (I-R) injury is greatly influenced by the testicular torsion/detorsion process (TDP). In this instance, the anti-inflammatory properties of plateletrich plasma (PRP) combined with tadalafil (Td) significantly promote tissue healing in the I-R injury model.

Methods: Five groups of rats were created: the control group, the I-R group not receiving any therapy, the I-R group receiving a single dosage of Td (0.25 mg/kg, I.P.), the I-R group receiving a single dose of PRP (80 l, intratesticular), and the I-R group receiving both Td and PRP. Sperm morphology, motility, and histology were assessed. The levels of TNF-, BAX, antioxidant status, and testosterone were measured. Additionally, E-selectin expression was done.

Results: PRP reduced oxidative stress, inflammation, and apoptosis while also boosting testosterone levels, which alleviated I-R injury. Otherwise, PRP reduces E-selectin expression, which modifies the pathways that control endothelial function. Td also partially demonstrated its testicular-protective activity at the same time.

Conclusion: PRP's proven anti-inflammatory, antioxidant, and antiapoptotic potentials make it a natural treatment for testicular harm caused by tadalafil. For the first time, it was demonstrated that PRP therapy restored the functionality of the vascular endothelium, specifically the control of E-selectin expression. Combining Td and PRP therapy may be a promising strategy for improving response to PDE5 inhibitors.

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来源期刊
CiteScore
5.80
自引率
0.00%
发文量
86
审稿时长
1 months
期刊介绍: Cellular Physiology and Biochemistry is a multidisciplinary scientific forum dedicated to advancing the frontiers of basic cellular research. It addresses scientists from both the physiological and biochemical disciplines as well as related fields such as genetics, molecular biology, pathophysiology, pathobiochemistry and cellular toxicology & pharmacology. Original papers and reviews on the mechanisms of intracellular transmission, cellular metabolism, cell growth, differentiation and death, ion channels and carriers, and the maintenance, regulation and disturbances of cell volume are presented. Appearing monthly under peer review, Cellular Physiology and Biochemistry takes an active role in the concerted international effort to unravel the mechanisms of cellular function.
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