在活细胞核内原位合成量子点

IF 16.3 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

National Science Review Pub Date : 2024-01-11 DOI:10.1093/nsr/nwae021

Yusi Hu, Zhi-Gang Wang, Haohao Fu, Chuanzheng Zhou, Wensheng Cai, Xueguang Shao, Shu-Lin Liu, Dai-Wen Pang

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引用次数: 0

摘要

细胞核是储存和复制遗传物质的主要场所，细胞核内物质的合成具有节律性、规律性，并受到生理过程的严格调控。然而，外源物质（如纳米粒子）能否在活细胞核内原位合成尚未见报道。在这里，我们通过调控谷胱甘肽（GSH）代谢途径，在细胞核中实现了 CdSxSe1-x 量子点（QDs）的原位合成。在Bcl-2蛋白的帮助下，通过添加GSH可以实现细胞核中GSH的高度富集。然后，高价硒被谷胱甘肽还原酶（GR）催化的GSH还原成低价硒，并与通过镉和富硫醇蛋白形成的镉前体相互作用，最终在细胞核中生成QDs。我们的工作有助于对细胞核内物质合成的新认识，并将为开发先进的 "超级细胞 "铺平道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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In-situ synthesis of quantum dots in the nucleus of live cells

Cell nucleus is the main site for the storage and replication of genetic material, and the synthesis of substances in the nucleus is rhythmic, regular, and strictly regulated with physiological processes. However, whether exogenous substances, such as nanoparticles, can be synthesized in situ in the nucleus of live cells has not been reported. Here, we have achieved in-situ synthesis of CdSxSe1−x quantum dots (QDs) in the nucleus by regulation of the glutathione (GSH) metabolic pathway. High enrichment of GSH in the nucleus can be accomplished by the addition of GSH with the help of the Bcl-2 protein. Then, high-valence Se is reduced to low-valence Se by glutathione reductase (GR)-catalyzed GSH, and interacts with Cd precursor formed through Cd and thiol-rich proteins, eventually generating QDs in the nucleus. Our work contributes to a new understanding of the syntheses of substances in cell nucleus and will pave the way to the development of advanced ‘supercells’.

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来源期刊

National Science Review MULTIDISCIPLINARY SCIENCES-

CiteScore

24.10

自引率

1.90%

发文量

249

审稿时长

13 weeks

期刊介绍： National Science Review (NSR; ISSN abbreviation: Natl. Sci. Rev.) is an English-language peer-reviewed multidisciplinary open-access scientific journal published by Oxford University Press under the auspices of the Chinese Academy of Sciences.According to Journal Citation Reports, its 2021 impact factor was 23.178. National Science Review publishes both review articles and perspectives as well as original research in the form of brief communications and research articles.