HDAC1 介导的 NEU1 下调会加剧宫颈癌的侵袭性

IF 1.5 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-01-01 DOI:10.1615/critreveukaryotgeneexpr.2023051396

Nanzi Xie, Sisi Mei, Changlan Dai, Wei Chen

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引用次数: 0

摘要

HDAC1 在多类型癌症中发挥着癌基因的作用。本研究旨在探讨 HDAC1 在宫颈癌（CC）中的作用。使用相应的试剂盒测定临床样本中线粒体的能量代谢和氧化应激。蛋白-蛋白复合物采用 Co-IP 法进行分析。通过 ChIP 检测确认了 NRF2 和 NEU1 之间的结合位点。用 CCK-8 检测细胞活力。细胞增殖采用 CCK-8 和菌落形成检测法。细胞迁移和侵袭能力通过透孔试验进行测定。我们发现 HDAC1 在 CC 中上调。TSA 处理抑制了线粒体能量代谢，并减少了菌落数量以及迁移和侵袭细胞的数量。此外，HDAC1与NRF2相互作用，下调NEU1的表达。敲除 NEU1 可减轻 TSA 的作用，并增强 CC 细胞的侵袭性。总之，HDAC1在CC中起着癌基因的作用。靶向HDAC1可能是治疗CC的另一种策略。

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HDAC1-mediated downregulation of NEU1 exacerbates the aggressiveness of cervical cancer

HDAC1 functions as an oncogene in multi-type cancers. This study aimed to investigate the roles of HDAC1 in cervical cancer (CC). mRNA expression was determined using RT-PCR. Mitochondrial energy metabolism and oxidative stress in clinical samples were determined using corresponding kits. The protein–protein complexes was analyzed using Co-IP assay. The binding sites between NRF2 and NEU1 were confirmed by ChIP assay. Cell viability was detected by CCK-8. Cell proliferation was measured using CCK-8 and colony formation assays. Cell migrative and invasive ability were determined using transwell assay. We found that HDAC1 was upregulated in CC. TSA treatment suppressed mitochondrial energy metabolism, as well as decreased the number of colonies and migrated and invaded cells. Moreover, HDAC1 interacted with NRF2 to downregulate NEU1 expression. NEU1 knockdown attenuated the effects of TSA and enhanced the aggressiveness of CC cells. In conclusion, HDAC1 functions as an oncogene in CC. Targeting HDAC1 may be an alternative strategy for CC.

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来源期刊

Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.