Zhen Huang, Caixia Wang, Huixue Li, Yuming Zhou, Z. Duan, Yixue Bao, Qin Hu, Charles A. Powell, Baoshan Chen, Jisen Zhang, Muqing Zhang, Wei Yao
{"title":"来自囊壳镰刀菌的小分泌效应蛋白通过抑制 ScPi21 诱导的细胞死亡来抑制宿主免疫反应","authors":"Zhen Huang, Caixia Wang, Huixue Li, Yuming Zhou, Z. Duan, Yixue Bao, Qin Hu, Charles A. Powell, Baoshan Chen, Jisen Zhang, Muqing Zhang, Wei Yao","doi":"10.1111/mpp.13414","DOIUrl":null,"url":null,"abstract":"Abstract Fusarium sacchari is one of the primary pathogens causing pokkah boeng disease, which impairs the yield and quality of sugarcane around the world. Understanding the molecular mechanisms of the F. sacchari effectors that regulate plant immunity is of great importance for the development of novel strategies for the persistent control of pokkah boeng disease. In a previous study, Fs00367 was identified to inhibit BAX‐induced cell death. In this study, Fs00367nsp (without signal peptide) was found to suppress BAX‐induced cell death, reactive oxygen species bursts and callose accumulation. The amino acid region 113–142 of Fs00367nsp is the functional region. Gene mutagenesis indicated that Fs00367 is important for the full virulence of F. sacchari. A yeast two‐hybrid assay revealed an interaction between Fs00367nsp and sugarcane ScPi21 in yeast that was further confirmed using bimolecular fluorescence complementation, pull‐down assay and co‐immunoprecipitation. ScPi21 can induce plant immunity, but this effect could be blunted by Fs00367nsp. These results suggest that Fs00367 is a core pathogenicity factor that suppresses plant immunity through inhibiting ScPi21‐induced cell death. The findings of this study provide new insights into the molecular mechanisms of effectors in regulating plant immunity.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Small secreted effector protein from Fusarium sacchari suppresses host immune response by inhibiting ScPi21‐induced cell death\",\"authors\":\"Zhen Huang, Caixia Wang, Huixue Li, Yuming Zhou, Z. Duan, Yixue Bao, Qin Hu, Charles A. Powell, Baoshan Chen, Jisen Zhang, Muqing Zhang, Wei Yao\",\"doi\":\"10.1111/mpp.13414\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Fusarium sacchari is one of the primary pathogens causing pokkah boeng disease, which impairs the yield and quality of sugarcane around the world. Understanding the molecular mechanisms of the F. sacchari effectors that regulate plant immunity is of great importance for the development of novel strategies for the persistent control of pokkah boeng disease. In a previous study, Fs00367 was identified to inhibit BAX‐induced cell death. In this study, Fs00367nsp (without signal peptide) was found to suppress BAX‐induced cell death, reactive oxygen species bursts and callose accumulation. The amino acid region 113–142 of Fs00367nsp is the functional region. Gene mutagenesis indicated that Fs00367 is important for the full virulence of F. sacchari. A yeast two‐hybrid assay revealed an interaction between Fs00367nsp and sugarcane ScPi21 in yeast that was further confirmed using bimolecular fluorescence complementation, pull‐down assay and co‐immunoprecipitation. ScPi21 can induce plant immunity, but this effect could be blunted by Fs00367nsp. These results suggest that Fs00367 is a core pathogenicity factor that suppresses plant immunity through inhibiting ScPi21‐induced cell death. The findings of this study provide new insights into the molecular mechanisms of effectors in regulating plant immunity.\",\"PeriodicalId\":18763,\"journal\":{\"name\":\"Molecular plant pathology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular plant pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1111/mpp.13414\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular plant pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/mpp.13414","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Small secreted effector protein from Fusarium sacchari suppresses host immune response by inhibiting ScPi21‐induced cell death
Abstract Fusarium sacchari is one of the primary pathogens causing pokkah boeng disease, which impairs the yield and quality of sugarcane around the world. Understanding the molecular mechanisms of the F. sacchari effectors that regulate plant immunity is of great importance for the development of novel strategies for the persistent control of pokkah boeng disease. In a previous study, Fs00367 was identified to inhibit BAX‐induced cell death. In this study, Fs00367nsp (without signal peptide) was found to suppress BAX‐induced cell death, reactive oxygen species bursts and callose accumulation. The amino acid region 113–142 of Fs00367nsp is the functional region. Gene mutagenesis indicated that Fs00367 is important for the full virulence of F. sacchari. A yeast two‐hybrid assay revealed an interaction between Fs00367nsp and sugarcane ScPi21 in yeast that was further confirmed using bimolecular fluorescence complementation, pull‐down assay and co‐immunoprecipitation. ScPi21 can induce plant immunity, but this effect could be blunted by Fs00367nsp. These results suggest that Fs00367 is a core pathogenicity factor that suppresses plant immunity through inhibiting ScPi21‐induced cell death. The findings of this study provide new insights into the molecular mechanisms of effectors in regulating plant immunity.
期刊介绍:
Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.