{"title":"揭开病毒逃逸的神秘面纱:预凝和膜过滤中微絮结合病毒的定量分析","authors":"M. Yasui, Hiroyuki Katayama","doi":"10.2166/wh.2024.233","DOIUrl":null,"url":null,"abstract":"\n \n The implementation of precoagulation before the physical removal process is expected to achieve a high virus removal rate. However, viruses may form small flocs and subsequently escape into the effluent during physical removal processes. This study evaluated how viruses in the microflocs could be quantified using conventional virus quantification methods (plaque assay and quantitative polymerase chain reaction (qPCR)) to reveal the risk of underestimating virus concentration. In this study, the microfloc dissolution phenomenon in phosphate buffer solution was employed as a floc dissolution test. Viruses in microflocs formed under the experimental conditions, assuming that water treatments were quantified before and after floc dissolution. The findings revealed that virus concentrations increased by 1.0–3.9 log plaque-forming units/mL according to the plaque assay and by 1.7–4.0 log copies/mL according to the qPCR. This increase occurred after the dissolution of microflocs that were prepared in the humic acid test water. In the case of treated wastewater, virus concentrations increased in all samples according to the plaque assay and in seven of eight samples according to the qPCR. Our results indicate the necessity of careful consideration of virus quantification after precoagulation and physical removal systems.","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"5 11","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Unveiling the viral escape: quantification of microfloc-bound viruses in precoagulation and membrane filtration\",\"authors\":\"M. Yasui, Hiroyuki Katayama\",\"doi\":\"10.2166/wh.2024.233\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n \\n The implementation of precoagulation before the physical removal process is expected to achieve a high virus removal rate. However, viruses may form small flocs and subsequently escape into the effluent during physical removal processes. This study evaluated how viruses in the microflocs could be quantified using conventional virus quantification methods (plaque assay and quantitative polymerase chain reaction (qPCR)) to reveal the risk of underestimating virus concentration. In this study, the microfloc dissolution phenomenon in phosphate buffer solution was employed as a floc dissolution test. Viruses in microflocs formed under the experimental conditions, assuming that water treatments were quantified before and after floc dissolution. The findings revealed that virus concentrations increased by 1.0–3.9 log plaque-forming units/mL according to the plaque assay and by 1.7–4.0 log copies/mL according to the qPCR. This increase occurred after the dissolution of microflocs that were prepared in the humic acid test water. In the case of treated wastewater, virus concentrations increased in all samples according to the plaque assay and in seven of eight samples according to the qPCR. Our results indicate the necessity of careful consideration of virus quantification after precoagulation and physical removal systems.\",\"PeriodicalId\":17436,\"journal\":{\"name\":\"Journal of water and health\",\"volume\":\"5 11\",\"pages\":\"\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-01-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of water and health\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.2166/wh.2024.233\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of water and health","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.2166/wh.2024.233","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Unveiling the viral escape: quantification of microfloc-bound viruses in precoagulation and membrane filtration
The implementation of precoagulation before the physical removal process is expected to achieve a high virus removal rate. However, viruses may form small flocs and subsequently escape into the effluent during physical removal processes. This study evaluated how viruses in the microflocs could be quantified using conventional virus quantification methods (plaque assay and quantitative polymerase chain reaction (qPCR)) to reveal the risk of underestimating virus concentration. In this study, the microfloc dissolution phenomenon in phosphate buffer solution was employed as a floc dissolution test. Viruses in microflocs formed under the experimental conditions, assuming that water treatments were quantified before and after floc dissolution. The findings revealed that virus concentrations increased by 1.0–3.9 log plaque-forming units/mL according to the plaque assay and by 1.7–4.0 log copies/mL according to the qPCR. This increase occurred after the dissolution of microflocs that were prepared in the humic acid test water. In the case of treated wastewater, virus concentrations increased in all samples according to the plaque assay and in seven of eight samples according to the qPCR. Our results indicate the necessity of careful consideration of virus quantification after precoagulation and physical removal systems.
期刊介绍:
Journal of Water and Health is a peer-reviewed journal devoted to the dissemination of information on the health implications and control of waterborne microorganisms and chemical substances in the broadest sense for developing and developed countries worldwide. This is to include microbial toxins, chemical quality and the aesthetic qualities of water.