血小板衍生的细胞外囊泡与尿毒症患者的肾损伤有关。

IF 2.3 3区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS
Zepeng Zhu , Xiaofeng Zhu , Dong Wang , Xun Lu , Tiancheng Jiang , Lei Zhang , Ming Chen , Aibing Yao , Shuqiu Chen
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引用次数: 0

摘要

背景:越来越多的证据表明,血小板衍生的细胞外囊泡(PEVs)可能参与了败血症的炎症风暴和器官损伤机制。然而,目前还没有关于尿毒症患者体内 PEVs 和肾损伤的研究:我们通过流式细胞术分析了血浆中 PEV 的浓度和比例,并通过 ELISA 检测了血浆中 IL-1β/IL-6/TNF-α/NGAL 的水平。此外,还采用了相关性分析来研究 PEVs 浓度与炎症因子和肾损伤指标水平以及疾病严重程度的关系。最后,绘制了PEVs浓度作为S-AKI/AKI诊断指标的接收者操作特征曲线:结果:我们发现泌尿生殖系统败血症患者的 IL-1β/IL-6/TNF-α/NGAL 水平明显较高。此外,泌尿系统败血症患者血浆中的 PEVs 浓度明显升高,尤其是革兰氏阴性菌感染患者,与 IL-1β/IL-6/TNF-α/NGAL 水平呈显著正相关。PEV诊断S-AKI和AKI的曲线下面积分别为0.746 [0.484, 1.000]和0.943 [0.874, 1.000]:总之,本研究表明,PEVs 可介导尿毒症患者体内炎症介质的释放,参与急性肾损伤的机制,并有可能成为 S-AKI 和 AKI 的诊断指标,以及尿毒症患者病情严重程度的早期预警指标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Platelet-derived extracellular vesicles are associated with kidney injury in patients with urosepsis

Background

There is increasing evidence that platelet-derived extracellular vesicles (PEVs) may be involved in the mechanisms of inflammatory storm and organ damage in sepsis. However, there are no available studies on PEVs and renal injury in patients with urosepsis.

Methods

We analyzed the concentration and ratio of PEVs in plasma by flow cytometry and measured plasma IL-1β/IL-6/TNF-α/NGAL levels by ELISA. Correlation analysis was also used to examine the concentration of PEVs in relation to levels of inflammatory factors and indicators of kidney damage, as well as the severity of the disease. Finally, the receiver operating characteristic curves were produced for PEVs concentrations as a diagnosis of S-AKI/AKI.

Results

We found significantly higher levels of IL-1β/IL-6/TNF-α/NGAL in patients with urogenital sepsis. Furthermore, the concentrations of PEVs in plasma were significantly elevated in patients with urosepsis, especially in patients with Gram-negative bacterial infections, which were significantly and positively correlated with IL-1β/IL-6/TNF-α/NGAL levels. The area under the curve for PEVs diagnosing S-AKI and AKI was 0.746 [0.484, 1.000] and 0.943 [0.874, 1.000] respectively.

Conclusion

Overall, the present study suggested that PEVs may mediate the release of inflammatory mediators in patients with urosepsis and participate in the mechanism of acute kidney injury, as well as having potential as diagnostic indicators of S-AKI and AKI and as early warning indicators of the severity of patients with urosepsis.

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来源期刊
Molecular and Cellular Probes
Molecular and Cellular Probes 生物-生化研究方法
CiteScore
6.80
自引率
0.00%
发文量
52
审稿时长
16 days
期刊介绍: MCP - Advancing biology through–omics and bioinformatic technologies wants to capture outcomes from the current revolution in molecular technologies and sciences. The journal has broadened its scope and embraces any high quality research papers, reviews and opinions in areas including, but not limited to, molecular biology, cell biology, biochemistry, immunology, physiology, epidemiology, ecology, virology, microbiology, parasitology, genetics, evolutionary biology, genomics (including metagenomics), bioinformatics, proteomics, metabolomics, glycomics, and lipidomics. Submissions with a technology-driven focus on understanding normal biological or disease processes as well as conceptual advances and paradigm shifts are particularly encouraged. The Editors welcome fundamental or applied research areas; pre-submission enquiries about advanced draft manuscripts are welcomed. Top quality research and manuscripts will be fast-tracked.
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