弱酸对致病病毒的杀毒作用取决于酸碱度。

IF 3.6 Q1 TROPICAL MEDICINE
Weiyin Hu, Hiroshi Shimoda, Yoshihiro Tsuchiya, Mikiya Kishi, Daisuke Hayasaka
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引用次数: 0

摘要

背景:弱酸(如醋酸)对病毒有杀灭作用,消毒剂之所以被认为是有效的杀病毒剂,可能是因为其 pH 值较低,取决于质子浓度。本研究旨在通过比较不同弱酸(乙酸、草酸和柠檬酸)和合格醋在不同 pH 值条件下对包膜和非包膜病毒的灭活效果,评估它们的功效:方法:将醋酸、草酸和柠檬酸的 pH 值分别调至 2、4 和 6。它们还用蒸馏水从 1 M 稀释到 0.001 M。有包膜的甲型流感病毒(FulV)、严重急性呼吸系统综合征冠状病毒 2(SARS-CoV-2)和无包膜的猫嗜热病毒(FCV)用调节后的弱酸处理长达 30 分钟。这些病毒还与白蒸馏醋(WDV)和谷物味蒸馏醋(GV)反应长达 30 分钟。反应后的感染性病毒滴度以每毫升斑块形成单位表示:结果:在 pH 值为 4 时,醋酸对 FulV 有杀毒作用,而柠檬酸和草酸没有。在 pH 值为 2 时,乙酸和柠檬酸能灭活 SARS-CoV-2,而草酸不能。所有酸在 pH 值为 2 时都对 FVC 有杀毒作用,但在 pH 值为 4 时则没有。1 分钟后,WDV 和 GV 能明显降低 FulV 滴度。SARS-CoV-2 也易受 WDV 和 GV 的杀毒作用影响,但培养时间延长至 30 分钟。相比之下,WDV 和 GV 对 FCV 的灭活效果并不明显:结论:即使在相同的 pH 值条件下,弱酸的灭活效果也是不同的,这表明弱酸的杀病毒效果并不仅仅由 pH 值决定,其他因素也可能影响这些效果。此外,主要成分为醋酸的合格醋可能是家庭环境中某些包膜病毒(如 FulV)的潜在消毒剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
pH-dependent virucidal effects of weak acids against pathogenic viruses.

Background: Weak acids, such as acetic acid, show virucidal effects against viruses, and disinfectants are considered effective virucidal agents possibly because of their low pH, depending on the proton concentration. This study aimed to evaluate the efficacy of different weak acids (acetic, oxalic, and citric acids) and eligible vinegars under different pH conditions by comparing their inactivation efficacies against enveloped and non-enveloped viruses.

Methods: Acetic, oxalic, and citric acids were adjusted to pH values of 2, 4 and 6, respectively. They were also diluted from 1 M to 0.001 M with distilled water. Enveloped influenza A virus (FulV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and non-enveloped feline calicivirus (FCV) were treated with adjusted weak acids for up to 30 min. These viruses were also reacted with white distilled vinegar (WDV) and grain-flavored distilled vinegar (GV) for up to 30 min. Infectious viral titers after the reactions were expressed as plaque-forming units per mL.

Results: Acetic acid showed virucidal effects against FulV at pH 4, whereas citric and oxalic acids did not. Acetic and citric acids inactivated SARS-CoV-2 at pH 2, whereas oxalic acid did not. All acids showed virucidal effects against FVC at pH 2; however, not at pH 4. The virucidal effects of the serially diluted weak acids were also reflected in the pH-dependent results. WDV and GV significantly reduced FulV titers after 1 min. SARS-CoV-2 was also susceptible to the virucidal effects of WDV and GV; however, the incubation period was extended to 30 min. In contrast, WDV and GV did not significantly inactivate FCV.

Conclusions: The inactivation efficacy of weak acids is different even under the same pH conditions, suggesting that the virucidal effect of weak acids is not simply determined by pH, but that additional factors may also influence these effects. Moreover, eligible vinegars, the main component of which is acetic acid, may be potential sanitizers for some enveloped viruses, such as FulV, in the domestic environment.

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来源期刊
Tropical Medicine and Health
Tropical Medicine and Health TROPICAL MEDICINE-
CiteScore
7.00
自引率
2.20%
发文量
90
审稿时长
11 weeks
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