利用新一代测序方法揭示城市影响下红树林栖息地细菌群落的作用

IF 2.1 4区 地球科学 Q2 MARINE & FRESHWATER BIOLOGY
Mayukhmita Ghose , Ashutosh Shankar Parab , Cathrine Sumathi Manohar, Deepika Mohanan, Ashwini Toraskar
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引用次数: 0

摘要

红树林生态系统是重要的沿海栖息地,具有丰富的生物多样性,在固碳方面发挥着重要作用,但这些栖息地正面临着快速城市化带来的日益严重的威胁。本研究深入评估了果阿Mandovi河口环境中Campal和Panaji城市红树林栖息地的沉积物细菌群落,以了解城市化与红树林栖息地细菌动态之间错综复杂的相互作用。我们采用 16S 扩增子和枪式测序相结合的方法来了解细菌的分类组成和功能特征。群落结构显示,这两个地点的细菌主要来自变形菌门、绿僵菌门和放线菌门。城市化的影响从该地区致病菌类和致病菌属数量的增加中可见一斑。核心微生物组分析表明,存在主要为致病菌的雪旺氏菌、假单胞菌和梭状芽孢杆菌,厌氧类群 Woeseia、Rheinheimera,以及参与有机物循环的类似菌属和未分类细菌。虽然报告的致病菌有所增加,但显而易见的是,优势类群也包括对这些地点沉积物中有机物的生物地球化学循环做出贡献的类群。通过散弹枪元基因组学分析阐明的功能多样性表明,碳水化合物代谢占主导地位,存在降解植物性淀粉、果胶和纤维素有机物的糖苷水解酶。异生物的生物降解途径表明,异生物降解微生物的存在是降解该地大量污染物的一种自然手段。这项研究的元基因组与早先在果阿的 Ribandar、Mandovi 和 Cortalim、Zuari 红树林栖息地进行的污染程度不同的类似研究进行的比较分析,也重申了陆地对这些栖息地微生物群落的影响。研究结果表明,帕纳吉和坎帕尔地区存在主要的致病菌,而其他两个地区则没有。据报道,人为活动对 Ribandar 和 Coratlim 地区的影响相对较小,但在这两个地区,参与再矿化的主要异养菌属的数量有所增加,病原体的数量则有所减少。我们的研究结果凸显了城市化对沉积物红树林细菌群落的深刻影响,并强调了这些微生物的恢复能力和适应能力。这就要求我们立即采取行动并采取预防措施,以恢复它们的功能潜力,在不断扩大的城市景观中维护这些生态系统的健康。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Unraveling the role of bacterial communities in mangrove habitats under the urban influence, using a next-generation sequencing approach

Unraveling the role of bacterial communities in mangrove habitats under the urban influence, using a next-generation sequencing approach

Unraveling the role of bacterial communities in mangrove habitats under the urban influence, using a next-generation sequencing approach

Mangrove ecosystems are vital coastal habitats with rich biodiversity and play a significant role in carbon sequestration, these habitats are facing mounting threats from rapid urbanization. This study delves into assessing the sediment bacterial communities from the urban mangrove habitats of Campal and Panaji, within the Mandovi estuarine environment of Goa, to understand the intricate interplay between urbanization and bacterial dynamics in the mangrove habitats. We used a combined approach of 16S amplicon and shotgun sequencing methodologies to understand the bacterial taxonomic composition and functional profiles. The community structure revealed the dominance of bacterial groups from phylum Proteobacteria, Chloroflexi and Actinobacteria at both locations. The impact of urbanization was evident from the increased abundance of pathogenic groups at class and genus levels in this region. The core microbiome analysis showed the presence of Shewanella, Pseudomonas and Clostridium which are mostly pathogenic, anaerobic taxa Woeseia, Rheinheimera, like genera that are involved in the organic matter cycling and unclassified bacteria. Though increased pathogenic forms are reported, it is evident that the dominant taxa also included groups that contributed to the biogeochemical cycling of organic matter in sediment from these locations. Functional diversity elucidated through shotgun metagenomics analysis showcased the predominance of carbohydrate metabolism and the presence of glycoside hydrolases degrading plant-based starch, pectin and cellulosic organic matter. The occurrence of xenobiotic biodegradation pathways is an indication of the presence of xenobiotic degrading microbes as a natural means of degrading high amounts of pollutants at this location. Comparative analysis of the metagenomes from this study with similar earlier studies at Ribandar, Mandovi and Cortalim, Zuari mangrove habitats of Goa with varying pollution levels also reiterates the terrestrial influence on the microbial community in these habitats. It showed that Panaji and Campal locations had the presence of dominant pathogenic forms which was absent in the other two locations. The Ribandar and Coratlim location which is reported to have a comparatively lesser impact due to anthropogenic activity had an increased abundance of distinct genera which are predominant heterotrophic forms involved in remineralization and reduced levels of pathogens. Our findings highlight the profound influence of urbanization on the sediment mangrove bacterial communities and spotlight the resilience and adaptability of these microorganisms. This calls for immediate action and preventive measures to restore their functional potential in maintaining the health of these ecosystems amidst expanding urban landscapes.

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来源期刊
Journal of Sea Research
Journal of Sea Research 地学-海洋学
CiteScore
3.20
自引率
5.00%
发文量
86
审稿时长
6-12 weeks
期刊介绍: The Journal of Sea Research is an international and multidisciplinary periodical on marine research, with an emphasis on the functioning of marine ecosystems in coastal and shelf seas, including intertidal, estuarine and brackish environments. As several subdisciplines add to this aim, manuscripts are welcome from the fields of marine biology, marine chemistry, marine sedimentology and physical oceanography, provided they add to the understanding of ecosystem processes.
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