旋转培养法制备的巨噬细胞聚集体的特征及其对聚合物材料的反应

IF 1.1 4区 医学 Q4 ENGINEERING, BIOMEDICAL
Journal of Artificial Organs Pub Date : 2024-12-01 Epub Date: 2024-01-09 DOI:10.1007/s10047-023-01428-6
Shota Toda, Yoshihide Hashimoto, Naoko Nakamura, Masahiro Yamada, Ryusuke Nakaoka, Wataru Nomura, Masaya Yamamoto, Tsuyoshi Kimura, Akio Kishida
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引用次数: 0

摘要

了解巨噬细胞与生物材料之间的相互作用对于创造新的生物材料和开发控制巨噬细胞功能的技术非常重要。由于巨噬细胞具有很强的粘附性,因此在进行体外评估时必须谨慎。同样,当使用磷脂醇-12-肉豆蔻酸-13-醋酸酯(PMA)将巨噬细胞前体细胞 THP-1 分化为巨噬细胞时,很难将其从粘附的基质上分离出来,这一直是研究生物材料免疫反应的一个难题。本研究基于一种新的 THP-1 细胞播种方法,分析了 THP-1 细胞分化巨噬细胞与生物材料的相互作用。在无 PMA 和有 PMA 的情况下,对 THP-1 细胞进行静态和旋转培养。在未分化的 THP-1 细胞中,静态培养和旋转培养的细胞功能没有变化。在含有 PMA 的旋转培养中,THP-1 细胞分化并形成巨噬细胞聚集体。分化和 M1/M2 极化后,检测了巨噬细胞聚集体中 IL-1β 和 MRC1 的表达。与静态培养的巨噬细胞相比,旋转培养的巨噬细胞聚集体倾向于向 M2 巨噬细胞极化。在评估巨噬细胞聚集体对几种聚合物材料的反应时,巨噬细胞聚集体在 30、50 和 70 rpm 转速下的 MRC1 表达随着时间的推移出现了不同的变化。30 rpm 的转速被认为是最合适的条件,因为它能得到与静态培养相同趋势的稳定结果。利用旋转培养获得的巨噬细胞聚集体有望为评估生物材料的炎症特性提供新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Characteristics of macrophage aggregates prepared by rotation culture and their response to polymeric materials.

Characteristics of macrophage aggregates prepared by rotation culture and their response to polymeric materials.

Understanding the interaction between macrophages and biomaterials is important for the creation of new biomaterials and the development of technologies to control macrophage function. Since macrophages are strongly adhesive, caution is required when performing in vitro evaluations. Similarly, when THP-1 cells, macrophage precursor cells, are differentiated into macrophages using phorbol-12-myristate-13-acetate (PMA), it becomes difficult to detach them from the adherent substrate, which has been a problem on investigation of immunological responses to biomaterials. In this study, the interaction of THP-1 cell-differentiated macrophages with biomaterials was analyzed based on a new method of seeding THP-1 cells. THP-1 cells were cultured in static and rotation culture without and with PMA. In undifferentiated THP-1 cells, there was no change in cellular function between static and rotation cultures. In rotation culture with PMA, THP-1 cells differentiated and formed macrophage aggregates. IL-1β and MRC1 expression in macrophage aggregates was examined after differentiation and M1/M2 polarization. Macrophage aggregates in rotation culture tended to be polarized toward M2 macrophages compared with those in static culture. In the evaluation of the responses of macrophage aggregates to several kinds of polymeric materials, macrophage aggregates showed different changes in MRC1 expression over time at 30, 50, and 70 rpm. Rotation speed of 30 rpm was considered most appropriate condition in that it gave stable results with the same trend as obtained with static culture. The use of macrophage aggregates obtained by rotational culture is expected to provide new insights into the evaluation of inflammatory properties of biomaterials.

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来源期刊
Journal of Artificial Organs
Journal of Artificial Organs 医学-工程:生物医学
CiteScore
2.80
自引率
15.40%
发文量
68
审稿时长
6-12 weeks
期刊介绍: The aim of the Journal of Artificial Organs is to introduce to colleagues worldwide a broad spectrum of important new achievements in the field of artificial organs, ranging from fundamental research to clinical applications. The scope of the Journal of Artificial Organs encompasses but is not restricted to blood purification, cardiovascular intervention, biomaterials, and artificial metabolic organs. Additionally, the journal will cover technical and industrial innovations. Membership in the Japanese Society for Artificial Organs is not a prerequisite for submission.
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