{"title":"用 G-CSF 和富血小板血浆（PRP）治疗化疗药物引起的雌性大鼠卵巢损伤：与新型标记物 INSL-3 相关的免疫组化研究。","authors":"Caglar Cetin, Sabri Berkem Okten, Olgu Enis Tok, Pınar Ozcan, Ayse Filiz Gokmen Karasu, Fatma Basak Tanoglu, Havva Sevde Taha, Seda Ates","doi":"10.1080/09513590.2023.2301551","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To assess the impacts of Platelet-Rich Plasma(PRP) and Granulocyte Colony-Stimulating Factor(G-CSF) on a rat model with induced ovarian follicular damage caused by cyclophosphamide(Cy).</p><p><strong>Materials and methods: </strong>Forty-two Sprague-Dawley rats were randomly allocated into seven distinct groups as; <b>Group 1(control):</b> NaCl intraperitoneal (IP) injection was administered on days D1, D7, and D14. <b>Group 2(Cy):</b>Cy IP injection on D1 + NaCl IP injection on D7 and D14 were administered. <b>Group 3(PRP):</b> PRP IP injection on D1,D7 and D14 were administered. <b>Group 4(Cy + PRP):</b>Cy IP injection on D1 and PRP IP injection on D1, D7 and D14 were administered. <b>Group 5(G-CSF):</b> G-CSF IP injection on D1, D7 and D14 were administered. <b>Group 6(Cy + G-CSF):</b>Cy IP injection on D1+ G-CSF IP injection on D1, D7 and D14 were administered. <b>Group 7(Cy + PRP + G-CSF):</b>Cy IP injection on D1+ PRP IP injection on D1,D7 and D14+ G-CSF IP injection on D1,D7 and D14 were administered. Follicular number, histological scores of AMH and INSL3 stained follicles at different stages of follicular development, and serum Anti-Müllerian hormone(AMH) were evaluated.</p><p><strong>Results: </strong>The primary, secondary, and antral follicle intensity scores for AMH-positive staining were most prominent in Groups 3 and 5. There was no significant difference between groups 4, 6 and 7 compared to group 1 in terms of follicule counts and AMH staining. The intensity scores of AMH-positive staining follicles were notably reduced in group 2 compared to groups 4, 6, and 7, with a significant difference (<i>p</i> < .01). Among the groups, group 2 exhibited the least intense antral follicle staining for INSL3, displaying a significant difference(<i>p</i> < .01) compared to the remaining groups.</p><p><strong>Conclusions: </strong>Autologous PRP and G-CSF might protect ovarian function in the face of ovarian damage caused by Cy-induced effects.</p>","PeriodicalId":12865,"journal":{"name":"Gynecological Endocrinology","volume":"40 1","pages":"2301551"},"PeriodicalIF":2.0000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Treatment of ovarian damage induced by chemotherapeutic drugs in female rats with G-CSF and platelet-rich plasma(PRP): an immunohistochemical study correlation with novel marker INSL-3.\",\"authors\":\"Caglar Cetin, Sabri Berkem Okten, Olgu Enis Tok, Pınar Ozcan, Ayse Filiz Gokmen Karasu, Fatma Basak Tanoglu, Havva Sevde Taha, Seda Ates\",\"doi\":\"10.1080/09513590.2023.2301551\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To assess the impacts of Platelet-Rich Plasma(PRP) and Granulocyte Colony-Stimulating Factor(G-CSF) on a rat model with induced ovarian follicular damage caused by cyclophosphamide(Cy).</p><p><strong>Materials and methods: </strong>Forty-two Sprague-Dawley rats were randomly allocated into seven distinct groups as; <b>Group 1(control):</b> NaCl intraperitoneal (IP) injection was administered on days D1, D7, and D14. <b>Group 2(Cy):</b>Cy IP injection on D1 + NaCl IP injection on D7 and D14 were administered. <b>Group 3(PRP):</b> PRP IP injection on D1,D7 and D14 were administered. <b>Group 4(Cy + PRP):</b>Cy IP injection on D1 and PRP IP injection on D1, D7 and D14 were administered. <b>Group 5(G-CSF):</b> G-CSF IP injection on D1, D7 and D14 were administered. <b>Group 6(Cy + G-CSF):</b>Cy IP injection on D1+ G-CSF IP injection on D1, D7 and D14 were administered. <b>Group 7(Cy + PRP + G-CSF):</b>Cy IP injection on D1+ PRP IP injection on D1,D7 and D14+ G-CSF IP injection on D1,D7 and D14 were administered. Follicular number, histological scores of AMH and INSL3 stained follicles at different stages of follicular development, and serum Anti-Müllerian hormone(AMH) were evaluated.</p><p><strong>Results: </strong>The primary, secondary, and antral follicle intensity scores for AMH-positive staining were most prominent in Groups 3 and 5. There was no significant difference between groups 4, 6 and 7 compared to group 1 in terms of follicule counts and AMH staining. The intensity scores of AMH-positive staining follicles were notably reduced in group 2 compared to groups 4, 6, and 7, with a significant difference (<i>p</i> < .01). Among the groups, group 2 exhibited the least intense antral follicle staining for INSL3, displaying a significant difference(<i>p</i> < .01) compared to the remaining groups.</p><p><strong>Conclusions: </strong>Autologous PRP and G-CSF might protect ovarian function in the face of ovarian damage caused by Cy-induced effects.</p>\",\"PeriodicalId\":12865,\"journal\":{\"name\":\"Gynecological Endocrinology\",\"volume\":\"40 1\",\"pages\":\"2301551\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gynecological Endocrinology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/09513590.2023.2301551\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/9 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"ENDOCRINOLOGY & METABOLISM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gynecological Endocrinology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/09513590.2023.2301551","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/9 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
引用次数: 0
摘要
目的评估血小板丰富血浆(PRP)和粒细胞集落刺激因子(G-CSF)对环磷酰胺(Cy)引起的大鼠卵泡损伤模型的影响：将42只Sprague-Dawley大鼠随机分为7组：第1组（对照组）：第 1 组（对照组）：在第 D1、D7 和 D14 天腹腔注射氯化钠（IP）。第 2 组（Cy）：D1 天 IP 注射 Cy + D7 天和 D14 天 IP 注射 NaCl。第 3 组（PRP）：D1、D7 和 D14 天 IP 注射 PRP。第 4 组（Cy + PRP）：D1 天 IP 注射 Cy，D1、D7 和 D14 天 IP 注射 PRP。第 5 组（G-CSF）：在 D1、D7 和 D14 进行 G-CSF IP 注射。第 6 组（Cy + G-CSF）：Cy IP 注射（D1）+ G-CSF IP 注射（D1、D7 和 D14）。第 7 组（Cy + PRP + G-CSF）：Cy IP 注射（D1）+ PRP IP 注射（D1、D7 和 D14）+ G-CSF IP 注射（D1、D7 和 D14）。对卵泡发育不同阶段的卵泡数量、AMH和INSL3染色卵泡的组织学评分以及血清抗缪勒氏管激素（AMH）进行了评估：第3组和第5组的原发、继发和前卵泡AMH阳性染色强度评分最为突出。第 4、6 和 7 组在卵泡数和 AMH 染色方面与第 1 组相比无明显差异。与第 4、6 和 7 组相比，第 2 组 AMH 阳性染色卵泡的强度评分明显降低，差异显著（p p 结论）：面对 Cy 诱导的卵巢损伤，自体 PRP 和 G-CSF 可保护卵巢功能。
Treatment of ovarian damage induced by chemotherapeutic drugs in female rats with G-CSF and platelet-rich plasma(PRP): an immunohistochemical study correlation with novel marker INSL-3.
Objective: To assess the impacts of Platelet-Rich Plasma(PRP) and Granulocyte Colony-Stimulating Factor(G-CSF) on a rat model with induced ovarian follicular damage caused by cyclophosphamide(Cy).
Materials and methods: Forty-two Sprague-Dawley rats were randomly allocated into seven distinct groups as; Group 1(control): NaCl intraperitoneal (IP) injection was administered on days D1, D7, and D14. Group 2(Cy):Cy IP injection on D1 + NaCl IP injection on D7 and D14 were administered. Group 3(PRP): PRP IP injection on D1,D7 and D14 were administered. Group 4(Cy + PRP):Cy IP injection on D1 and PRP IP injection on D1, D7 and D14 were administered. Group 5(G-CSF): G-CSF IP injection on D1, D7 and D14 were administered. Group 6(Cy + G-CSF):Cy IP injection on D1+ G-CSF IP injection on D1, D7 and D14 were administered. Group 7(Cy + PRP + G-CSF):Cy IP injection on D1+ PRP IP injection on D1,D7 and D14+ G-CSF IP injection on D1,D7 and D14 were administered. Follicular number, histological scores of AMH and INSL3 stained follicles at different stages of follicular development, and serum Anti-Müllerian hormone(AMH) were evaluated.
Results: The primary, secondary, and antral follicle intensity scores for AMH-positive staining were most prominent in Groups 3 and 5. There was no significant difference between groups 4, 6 and 7 compared to group 1 in terms of follicule counts and AMH staining. The intensity scores of AMH-positive staining follicles were notably reduced in group 2 compared to groups 4, 6, and 7, with a significant difference (p < .01). Among the groups, group 2 exhibited the least intense antral follicle staining for INSL3, displaying a significant difference(p < .01) compared to the remaining groups.
Conclusions: Autologous PRP and G-CSF might protect ovarian function in the face of ovarian damage caused by Cy-induced effects.
期刊介绍：
Gynecological Endocrinology , the official journal of the International Society of Gynecological Endocrinology, covers all the experimental, clinical and therapeutic aspects of this ever more important discipline. It includes, amongst others, papers relating to the control and function of the different endocrine glands in females, the effects of reproductive events on the endocrine system, and the consequences of endocrine disorders on reproduction
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