柠檬提取物通过上调 Nrf2 和 Trx1 的表达,降低老龄大鼠肝脏氧化应激和过硫化水平。

IF 5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
BioFactors Pub Date : 2024-01-09 DOI:10.1002/biof.2038
Marko Miler, Jasmina Živanović, Vladimir Ajdžanović, Dragan Milenkovic, Thais Cesar, Miloš R. Filipović, Verica Milošević
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引用次数: 0

摘要

在健康老龄化的概念中,柑橘黄酮被认为是一种很有前景的生物活性物质。因此,本研究调查了营养相关剂量的柠檬提取物(LE)对 24 个月大的 Wistar 大鼠肝脏氧化还原调节和过硫化水平的影响。大鼠每天口服一次柠檬提取物(40 毫克/千克体重),连续服用 4 周。对照组服用载体(葵花籽油)或保持原样。应用的方法包括 qPCR、Western 印迹、蛋白质过硫化水平评估、与免疫荧光一致的组织化学、肝脏生化检测(谷胱甘肽、总-SH 组和丙二醛;MDA)、血清中的肝酶,以及旨在探索本文所研究蛋白质之间潜在相互作用/结合的硅学分析。我们的研究结果表明,LE能分别增加谷胱甘肽过氧化物酶(GPx)、还原酶(GR)、谷氨酸-半胱氨酸连接酶催化亚基和修饰亚基以及Nrf2基因的表达,但降低了超氧化物歧化酶2(SOD2)的表达。应用LE后,NRF2、SOD2、GPx、GR和硫代氧化还蛋白1(Trx1)的蛋白表达出现上调。LE 能明显降低蛋白质的过硫化水平和细胞中氧化损伤标志物 MDA 的浓度。组织学分析表明,肝脏组织结构正常,无病理变化,血清中的肝酶水平正常。研究结果表明,柠檬黄酮通过调节肝脏氧化还原调节因子 Nrf2 和 Trx1,减轻了氧化应激并改变了过硫化水平,这表明柠檬黄酮在老年肝脏中具有相当大的有益抗氧化潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats

Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats

Citrus flavanones are recognized as promising bioactives within the concept of healthy aging. Thus, the present study investigated the effects of a nutritionally relevant dose of lemon extract (LE) on liver redox regulation and persulfidation levels in 24-month-old Wistar rats. LE (40 mg/kg b.m.) was administered orally once daily for 4 weeks. Control groups received either vehicle (sunflower oil) or remained intact. The applied methodology considered qPCR, Western blot, protein persulfidation levels evaluation, histochemistry in line with immunofluorescence, liver biochemical assays (glutathione, total -SH groups and malonaldehyde; MDA), liver enzymes in serum and in silico analysis to explore the potential interaction/binding between the proteins studied in the paper. Our results showed that LE increased glutathione peroxidase (GPx), reductase (GR), glutamate–cysteine ligase catalytic and modifier subunit, respectively, as well as Nrf2 gene expressions, but decreased the expression of superoxide dismutase 2 (SOD2). Upon LE application, protein expression showed upregulation of NRF2, SOD2, GPx, GR, and thioredoxin 1 (Trx1). LE significantly decreased the protein persulfidation levels and concentration of MDA, a marker of oxidative damage in the cell. Histological analysis showed a normal liver histoarchitecture without pathological changes, aligning with the normal serum level of hepatic enzymes. Obtained results showed that LE, by modulating hepatic redox regulators Nrf2 and Trx1, diminishes oxidative stress and alters the persulfidation levels, suggesting a considerable beneficial antioxidant potential of lemon flavanones in the old-aged liver.

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来源期刊
BioFactors
BioFactors 生物-内分泌学与代谢
CiteScore
11.50
自引率
3.30%
发文量
96
审稿时长
6-12 weeks
期刊介绍: BioFactors, a journal of the International Union of Biochemistry and Molecular Biology, is devoted to the rapid publication of highly significant original research articles and reviews in experimental biology in health and disease. The word “biofactors” refers to the many compounds that regulate biological functions. Biological factors comprise many molecules produced or modified by living organisms, and present in many essential systems like the blood, the nervous or immunological systems. A non-exhaustive list of biological factors includes neurotransmitters, cytokines, chemokines, hormones, coagulation factors, transcription factors, signaling molecules, receptor ligands and many more. In the group of biofactors we can accommodate several classical molecules not synthetized in the body such as vitamins, micronutrients or essential trace elements. In keeping with this unified view of biochemistry, BioFactors publishes research dealing with the identification of new substances and the elucidation of their functions at the biophysical, biochemical, cellular and human level as well as studies revealing novel functions of already known biofactors. The journal encourages the submission of studies that use biochemistry, biophysics, cell and molecular biology and/or cell signaling approaches.
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