解密 TIA-1 中与 Zn2+ 结合的组氨酸在应激颗粒的组装和动力学中的作用。

IF 5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
BioFactors Pub Date : 2024-01-09 DOI:10.1002/biof.2037
Laura Corrales-Guerrero, Irene Díaz-Moreno
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引用次数: 0

摘要

T 细胞胞内抗原-1(TIA-1)是一种关键的 RNA 结合蛋白,参与翻译调控和 RNA 剪接。TIA-1会发生液-液相分离,这是RNA和蛋白质凝结成称为应激颗粒(SG)的无膜细胞器的基本机制。然而,这种动态行为会导致异常纤维形成,与神经退行性疾病有关,因此必须加以严格调控。在本研究中,我们研究了组氨酸残基 His94 和 His96 在细胞中的作用,它们负责 Zn2+ 的结合。利用荧光显微镜,我们发现这些残基形成的特定结合位点对 SG 的组装至关重要。此外,它还起着维持 SG 组装的 TIA-1 动态行为的作用。总之,我们的研究结果证实了 TIA-1 His94 和 His96 在 Zn2+ 介导的调控机制中对防止 SG 纤维形成的生理作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Deciphering the role of Zn2+-binding histidines from TIA-1 on the assembly and dynamics of stress granules

Deciphering the role of Zn2+-binding histidines from TIA-1 on the assembly and dynamics of stress granules

T-cell intracellular antigen-1 (TIA-1) is a key RNA-binding protein that participates in translation regulation and RNA splicing. TIA-1 undergoes liquid–liquid phase separation as a fundamental mechanism that enables the condensation of RNA and proteins into membraneless organelles called stress granules (SGs). However, this dynamic behavior can lead to aberrant fibril formation, implicated in neurodegenerative disorders, and must be tightly regulated. In this study, we investigated the role in the cell of histidine residues His94 and His96, responsible for Zn2+ binding. Using fluorescence microscopy, we found that the specific binding site formed by these residues is critical for SG assembly. Furthermore, it also plays a role maintaining the dynamic behavior of SG-assembled TIA-1. Collectively, our findings confirm the physiological relevance of TIA-1 His94 and His96 in the Zn2+-mediated regulatory mechanism for protection against fibril formation in SGs.

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来源期刊
BioFactors
BioFactors 生物-内分泌学与代谢
CiteScore
11.50
自引率
3.30%
发文量
96
审稿时长
6-12 weeks
期刊介绍: BioFactors, a journal of the International Union of Biochemistry and Molecular Biology, is devoted to the rapid publication of highly significant original research articles and reviews in experimental biology in health and disease. The word “biofactors” refers to the many compounds that regulate biological functions. Biological factors comprise many molecules produced or modified by living organisms, and present in many essential systems like the blood, the nervous or immunological systems. A non-exhaustive list of biological factors includes neurotransmitters, cytokines, chemokines, hormones, coagulation factors, transcription factors, signaling molecules, receptor ligands and many more. In the group of biofactors we can accommodate several classical molecules not synthetized in the body such as vitamins, micronutrients or essential trace elements. In keeping with this unified view of biochemistry, BioFactors publishes research dealing with the identification of new substances and the elucidation of their functions at the biophysical, biochemical, cellular and human level as well as studies revealing novel functions of already known biofactors. The journal encourages the submission of studies that use biochemistry, biophysics, cell and molecular biology and/or cell signaling approaches.
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