SDS 和 H2O2 组合对气管支架发育的协同效应:使用山羊气管进行的体外研究

IF 3 Q3 MATERIALS SCIENCE, BIOMATERIALS
Dhihintia Jiwangga, F. Mahyudin, Gondo Mastutik, Estya Nadya Meitavany, Juliana, P. A. Wiratama
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引用次数: 0

摘要

目前,组织工程气管已被广泛用作严重呼吸系统疾病的气管替代生物移植物。在组织工程气管支架的开发过程中,体外研究起着至关重要的作用,研究人员可以通过体外研究评估支架设计和制造技术的有效性和安全性,然后再进行体内或临床试验。这项研究使用 SDS、H2O2 及其组合对山羊气管进行脱细胞处理。研究人员进行了各种定量和定性评估,包括组织学分析、免疫组化和生物力学测试。血色素和伊红染色评估了细胞含量,而黄绿素 O-快绿染色和马森三色染色则分别评估了糖胺聚糖含量和胶原分布。免疫组化分析侧重于检测 MHC-1 抗原的存在。拉伸强度测量用于评估脱细胞支架的生物力学特性。结果表明,结合使用 SDS 和 H2O2 对山羊气管进行脱细胞处理可获得细胞残留极少、毒性低、ECM 保存完好、拉伸强度和弹性高的支架。这种方法有望开发出功能性气管支架,有效解决严重的呼吸系统疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Synergistic Effects of SDS and H2O2 Combinations on Tracheal Scaffold Development: An In Vitro Study Using Goat Trachea
Currently, a tissue-engineered trachea has been popularly used as a biological graft for tracheal replacement in severe respiratory diseases. In the development of tissue-engineered tracheal scaffolds, in vitro studies play a crucial role in allowing researchers to evaluate the efficacy and safety of scaffold designs and fabrication techniques before progressing to in vivo or clinical trials. This research involved the decellularization of goat trachea using SDS, H2O2, and their combinations. Various quantitative and qualitative assessments were performed, including histological analysis, immunohistochemistry, and biomechanical testing. Hematoxylin and eosin staining evaluated the cellular content, while safranin O-fast green and Masson’s trichrome staining assessed glycosaminoglycan content and collagen distribution, respectively. The immunohistochemical analysis focused on detecting MHC-1 antigen presence. Tensile strength measurements were conducted to evaluate the biomechanical properties of the decellularized scaffolds. The results demonstrated that the combination of SDS and H2O2 for goat tracheal decellularization yielded scaffolds with minimal cellular remnants, low toxicity, preserved ECM, and high tensile strength and elasticity. This method holds promise for developing functional tracheal scaffolds to address severe respiratory diseases effectively.
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来源期刊
International Journal of Biomaterials
International Journal of Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
4.30
自引率
3.20%
发文量
50
审稿时长
21 weeks
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