以堇菜属(Fumariaceae)的一些特有种为例,说明地理隔离和核糖体类型

A. S. Sukhov, M. A. Mikhailova, E. Machs
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引用次数: 0

摘要

这项科学工作的主要课题是研究长期生态和生殖隔离条件下物种核型的特征。我们选择了三类特有物种。第一组是嗜石膏物种:^rydalis sangardanica Mikhailova、С. microphylla Mikhailova、С. gypsophyla Mikhailova。第二类是岩垫种:С. mira (Batalin) C. Y. Wu et H. Chuang 和 С. yazgulemica Mikhailova et Sochivko。yazgulemica Mikhailova et Sochivko。第三类是岩石上的地理隔离物种:macrocalyx Litv 和 С. rupestris Kotschy。这项工作的科学创新之处在于在研究堇菜属(Fumariaceae)物种可能杂交的领域中使用了遗传分析方法。在 Illumina MiSeq 平台上进行了 NGS(下一代测序)。以核核糖体 35S rDNA 基因的第一转录间隔(ITS1)作为标记区域。结果,每个样本都获得了一组 Zotu 多拷贝核糖体型(零半径操作分类单位),并在此基础上对样本进行了定性和定量比较。结果表明,三组样本中没有共同的核糖体型。所研究物种的核型数量从 1 个到 6 个不等,这可能与过去杂交的特殊性有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Geographic isolation and ribotypes on the example of some endemics of the genus Corydalis (Fumariaceae)
The main topic of this scientific work is the study of the characteristics of species ribotypes under conditions of long-term ecological and reproductive isolation. Three groups of endemics were selected. The first group is gypsophilic species: ^rydalis sangardanica Mikhailova, С. microphylla Mikhailova, С. gypsophyla Mikhailova. The second group is the rock cushion species: С. mira (Batalin) C. Y. Wu et H. Chuang, and С. yazgulemica Mikhailova et Sochivko. The third group is rocky, geographically isolated species: С. macrocalyx Litv, and С. rupestris Kotschy. The scientific novelty in this work is the use of genetic methods of analysis in the field of studying the possible hybridization of species of the genus Corydalis (Fumariaceae). NGS (Next-Generation Sequencing) was performed on the Illumina MiSeq platform. The first transcribed spacer (ITS1) of the nuclear ribosomal 35S rDNA gene was taken as a marker region. As a result, for each sample, a set of Zotu multicopy ribotypes (zero-radius operational taxonomic unit) was obtained, on the basis of which a qualitative and quantitative comparison of the samples was performed. It was shown that there are no common ribotypes in all three groups. The number of ribotypes in the studied species varies from one to six, which may be due to the peculiarities of hybridization in the past.
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