利用 rapidGISH 技术对六种豆科植物 Hedysarum L. 的基因组进行比较分析

O. Y. Yurkevich, T. E. Samatadze, I. Selyutina, S. I. Romashkina, A. R. Semenov, S. A. Zoshchuk, A. V. Amosova, O. Muravenko
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引用次数: 0

摘要

利用快速基因组原位杂交(rapidGISH)技术对六种绣线菊(Hedysarum)的基因组进行了比较分析,从而确定了常见的高度重复 DNA 序列,并显示了这些序列在所研究物种染色体上的分布模式。在 H. alpinum 和 H. theinum(均属于 Hedysarum 科)的染色体上发现了 H. flavescens(Hedysarum 科)基因组 DNA 的分散定位。在 Hedysarum 节的 H. neglectum 染色体上,观察到 H. flavescens 基因组 DNA 的聚类杂交信号。在 H. flavescens 和 H. theinum 的所有染色体上都发现了 H. alpinum 基因组 DNA 的定位。在 H. grandiflorum 和 H. dahuricum(均为多孔菌科)的染色体上,发现了 H. flavescens 和 H. alpinum 基因组 DNA 的小聚类和分散杂交信号。因此,在所研究的物种中,发现了其染色体组织中不同类型的共同高度重复 DNA 序列。我们的研究结果表明,Hedysarum 和 Multicaulia 两个科的物种存在共同的起源,但它们之间的关系程度不同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative analysis of genomes of six species of Hedysarum L. (Fabaceae) by the rapidGISH technique
A comparative analysis of genomes of six Hedysarum species was carried out using the rapid genomic in situ hybridization (rapidGISH) technique, which makes it possible to identify common highly repetitive DNA sequences, and also shows the patterns of their distribution on chromosomes of the studied species. Dispersed localization of genomic DNA of H. flavescens (sect. Hedysarum) was found on chromosomes of H. alpinum and H. theinum (both belonged to sect. Hedysarum). On chromosomes of H. neglectum from the section Hedysarum, clustered hybridization signals of genomic DNA of H. flavescens were observed. The localization of H. alpinum genomic DNA was found along the all chromosomes of H. flavescens and H. theinum. On chromosomes of H. grandiflorum and H. dahuricum (both from sect. Multicaulia), small clustered and dispersed hybridization signals of genomic DNA of H. flavescens and H. alpinum were found. Thus, in the studied species, common highly repetitive DNA sequences of different types of their chromosome organization were revealed. Our findings indicate the presence of common origin of the species from the sections Hedysarum and Multicaulia with different degree of their relationship.
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