Occurrence of bone sialoprotein-binding protein gene among clinical vancomycin-resistant Enterococcus faecium isolated from bone infections

Vancomycin resistant Enterococcus faecium can be found in the bone resulting human osteomyelitis infections, this could be controlled by some encoding genes that can express the bacterial adhesions, which aid in bacterial colonization for the host bone tissues. Hence, this research aimed to detect the occurrence of bbp gene in clinical vancomycin resistant E. faecium isolated from bone infections. After collection, the samples were isolated and identified using different manual and automated protocols. Furthermore, vanA and bbp genes were molecularly investigated. Results show that 60% of the identified E. faecium were vancomycin-resistant with distribution of 32% and 28% in postsurgery and hematogenous infections, respectively. The prevalence of vanA and bbp genes was 100% in 20 vavancomycin-resistant faecium isolates. These findings show that vanA and bbp expression may be associated with bone infections. We draw the conclusion from this study that vanA and bbp detection can be used as a marker for vavancomycin-resistant faecium in the bone invasion.