一家三级医院医学检验专业学生白大褂上的细菌污染

Kusumakshi Nayak, Seema Shetty, Akshaya T Poojary, Thejaswini Prabhu
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摘要

背景:白大褂可能是潜在的感染源,在医院和社区中传播致病菌方面发挥着巨大作用。研究目的本横断面研究旨在评估在诊断实验室工作的学生白大褂上的细菌污染情况,并确定其抗菌敏感性。研究方法从一家三甲医院各种诊断实验室工作的 96 名学生的白大褂袖子和口袋中收集拭子,并根据标准程序进行细菌分析。结果:在 96 件白大褂中,63 件白大褂受到细菌污染。与袖子相比,白大褂口袋的致病菌污染程度较高。仅从 13 件白大褂中分离出鲍曼不动杆菌、铜绿假单胞菌和金黄色葡萄球菌。大部分是表皮葡萄球菌,其次是黄体小球菌。所有分离出的金黄色葡萄球菌都对所用的抗生素敏感。少数鲍曼不动杆菌对氨苄西林和阿莫西林有抗药性。一个铜绿假单胞菌分离株对头孢哌酮产生了抗药性。结论在诊断实验室工作的学生的白大褂受到病原菌和非病原菌的污染,这可能会造成交叉感染,并增加院内感染的传播。对抗生素产生耐药性的细菌会导致治疗并发症。因此,应制定并遵循相关政策,通过遵循实验室常规做法(如洗手、经常清洗白大褂和消毒其他场所)来降低传染病传播的风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bacterial contamination on the white coats of medical laboratory students at a tertiary care hospital
Background: White coats might be a potential source of infection and play a huge role in transmitting pathogenic bacteria in hospital setup as well as in the community. Objective: This cross-sectional study was conducted to evaluate the bacterial contamination on white coats of students working in diagnostic laboratories and determine their antibacterial susceptibility. Methods: Swabs were collected from the sleeves and pockets of the white coat of 96 students working in various diagnostic labs in a tertiary care hospital and processed for bacteriological analysis based on standard procedures. Results: Among 96 white coats, bacterial contamination was observed in 63 white coats. Pockets of the white coats were highly contaminated with pathogenic bacteria than sleeves. Acinetobacter baumannii, Pseudomonas aeruginosa and Staphylococcus aureus were isolated only from 13 white coats. The majority were Staphylococcus epidermidis, followed by Micrococcus luteus. All isolates of S. aureus were susceptible to antibiotics used. Few isolates of A. baumannii were resistant to ampicillin and amoxicillin. One isolate of P. aeruginosa exhibited resistance to Cefoperazone. Conclusion: White coats of students working in diagnostic labs were contaminated with both pathogenic and nonpathogenic bacteria, which can cause cross contamination and increase the spread of nosocomial infection. Bacteria resistant to antibiotics can lead to treatment complications. Thus, policies should be prepared and followed to reduce the risk of infectious disease transmission by following routine laboratory practices such as hand washing, frequent cleaning of white coats and disinfecting other fomites.
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