小调控 RNA Rli82 参与了单核细胞增生李斯特菌的运动性和致病性过程

IF 0.5 4区 医学 Q4 MICROBIOLOGY
Chunhui Ji, Nengxiu Li, Jian Jiao, Yaoqiang Sun, Yufei Zuo, Xin Huang, Xiaoxing Huang, Zhiyuan Li, Yaling Li, Qingwen Leng, Xuepeng Cai, Q. Meng, Jun Qiao
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引用次数: 0

摘要

背景:单核细胞增生李斯特菌(LM)是一种细胞内兼性病原体,可导致人类和动物的食源性感染。为了在宿主细胞内入侵和繁殖,李斯特菌利用各种策略精确调节其基因表达并适应体内环境。研究目的研究 Rli82 sRNA 在 LM EGD-e 的运动性和致病性中的调控作用。方法:利用同源重组技术构建 Rli82 基因敲除突变株 LM-ΔRli82 和互补株 LM-ΔRli82/Rli82,并分别测定其运动性和致病性。此外,利用TargetRNA2软件预测了Rli82调控的潜在靶mRNA,并通过双质粒报告系统验证了靶mRNA与Rli82之间的相互作用。结果表明结果表明,与 LM EGD-e 和 LM-ΔRli82/Rli82 相比,LM-ΔRli82 在 25°C 时的运动能力明显增强,能产生更多的鞭毛。此外,与 LM EGD-e 和 LM-ΔRli82/Rli82 相比,LM-ΔRli82 感染小鼠的半数致死剂量(LD50)明显增加,这表明删除 Rli82 基因后 LM 的毒力减弱。此外,与 LM EGD-e 和 LM-ΔRli82/Rli82 相比,LM-ΔRli82 的 flaA mRNA 水平没有显著升高,但 flaA 蛋白水平显著升高,这表明 Rli82 可能在转录后水平上调节了 flaA mRNA 的翻译。结论综上所述,我们的研究结果首次揭示了 Rli82 sRNA 可能参与调控 flaA 蛋白的表达,从而影响 LM 的移动性和致病性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Small Regulatory RNA, Rli82, Is Involved in the Motility and Pathogenicity of Listeria monocytogenes
Background: Listeria monocytogenes (LM) is a facultative intracellular pathogen that causes food-borne infections in humans and animals. To invade and multiply within host cells, LM utilizes various strategies to precisely modulate its gene expression and to adapt to the in vivo environment. Objectives: To investigate the regulatory roles of Rli82 sRNA in the motility and pathogenicity of LM EGD-e. Methods: The Rli82 gene knock-out mutant strain, LM-ΔRli82, and the complementation strain, LM-ΔRli82/Rli82, were constructed using homologous recombination technology, and their motility and virulence, respectively, were determined. Moreover, the potential target mRNA regulated by Rli82 was predicted using TargetRNA2 software, and then the interaction between the target mRNA and Rli82 was verified by the two-plasmid reporter system. Results: The results showed that the motility of LM-ΔRli82 was significantly increased at 25°C, facilitated by the production of more flagella than LM EGD-e and LM-ΔRli82/Rli82. Furthermore, LD50 in LM-ΔRli82-infected mice was significantly increased as compared to LM EGD-e and LM-ΔRli82/Rli82, suggesting that the virulence of LM was weakened when the Rli82 gene was deleted. In addition, the mRNA level of flaA was not significantly elevated, but flaA protein was significantly higher in LM-ΔRli82 than in LM EGD-e and LM-ΔRli82/Rli82, suggesting that Rli82 might modulate the translation of flaA mRNA at the post-transcriptional level. Conclusions: Taken together, our findings for the first time revealed that Rli82 sRNA might be involved in the modulation of the expression of flaA protein, thereby influencing the mobility and pathogenicity of LM.
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来源期刊
CiteScore
1.30
自引率
0.00%
发文量
56
审稿时长
6-12 weeks
期刊介绍: Jundishapur Journal of Microbiology, (JJM) is the official scientific Monthly publication of Ahvaz Jundishapur University of Medical Sciences. JJM is dedicated to the publication of manuscripts on topics concerning all aspects of microbiology. The topics include medical, veterinary and environmental microbiology, molecular investigations and infectious diseases. Aspects of immunology and epidemiology of infectious diseases are also considered.
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