对Lepidium sativum Linn诱导易位杂合子的减数分裂分析

IF 0.6 Q4 ENVIRONMENTAL SCIENCES
G. Kumar, P. Pandey, A. Pandey, J. Yadav, N.K. Tiwari, S. Pandey
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引用次数: 0

摘要

目的:评估伽马射线对Lepidium sativum Linn.花粉母细胞和减数分裂后产物的细胞学影响,从而分离出易位杂合子和非整倍体配子。实验方法在实验过程中,研究了减数分裂阶段、染色体结构和花粉受精率等参数。将新鲜的花园芹种子有序地排列在塑料袋中,并分成四组。在勒克瑙(印度)国家植物研究所(NBRI)的花卉栽培实验室分别用 60Gy、150Gy、300Gy、450Gy 和 600Gy @ 7.247KGyh-1 对每组的新鲜种子进行辐照。伽马射线辐照源为钴-60。经过辐照的种子与各自的对照组一起播种,每组三份。结果对 300Gy 辐射下的花粉母细胞(PMCs)进行的减数分裂分析表明,存在易位杂合子。细胞学图解显示,在二分裂期和分裂后期 I 染色体的构型中,四价、二价和其他形式的染色体组合(如多价染色体)更胜一筹。诱导易位的杂合子在分裂期和无丝分裂期会出现粘连,并在无丝分裂期形成桥。由于染色体异常,与对照植株(98.20±0.16%)相比,花粉育性降低到 37.54±0.25%。解释由于易位杂合子是培育具有新型遗传组合的非整倍体后代的来源,因此当前的研究工作具有重要意义。关键词: 染色体异常染色体异常 异源杂合子 玉米鳞茎 减数分裂 花粉育性
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Meiotic analysis of induced translocation heterozygotes in Lepidium sativum Linn.
Aim: To assess the cytological effects of Gamma rays on the pollen mother cells and post meiotic products of Lepidium sativum Linn. which resulted in isolation of translocation heterozygote and aneuploid gametes. Methodology: During the experiment, parameters such as Meiotic stages, chromosomal structure, and pollen fertility were studied. Fresh seeds of Garden cress were orderly arranged in plastic and divided into four groups. The fresh seeds of each group were individually irradiated with 60Gy, 150Gy, 300Gy, 450Gy and 600Gy @ 7.247KGyh-1 in the Floriculture Laboratory of National Botanical Research Institute (NBRI), Lucknow (India). The source for gamma ray irradiation was Cobalt-60. The irradiated seeds were sown in triplicates along with their respective controls. Results: Meiotic analysis of pollen mother cells (PMCs) at 300Gy showed translocation heterozygotes, however, in the control sets, the rate was negligible and phenotypically the plants were weak and short heighted. The cytological illustration of chromosomal configuration at diakinesis and metaphase I exhibited the superiority of tetravalents, bivalent and other forms of chromosomal associations such as multivalents. The induced translocation heterozygotes resulted in stickiness at metaphase and anaphase states along with the formation of bridges at anaphase stage. Due to chromosomal anomalies, the pollen fertility was reduced to 37.54±0.25% in contrast to control plants (98.20±0.16%). Interpretation: The current research work would be of paramount interest as translocation heterozygote serves as a source for raising aneuploid offspring having novel genetic combinations. Key words: Chromosomal anomalies, Heterozygotes, Lepidium sativum Linn, Meiosis, Pollen fertility
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来源期刊
Journal of environmental biology
Journal of environmental biology ENVIRONMENTAL SCIENCES-
CiteScore
1.70
自引率
0.00%
发文量
92
审稿时长
3 months
期刊介绍: Information not localized
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