马来西亚非怀孕成年人中侵袭性 B 组链球菌的疾病负担、抗菌药耐药性和分子特征：协议研究

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-11-01 DOI:10.4103/1995-7645.390165

AbdulRahman Muthanna, N. Baharin, Mohd Nasir Desa, N. Dzaraly, Mohammad Noor Azmai, Syafinaz Amin-Nordin

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引用次数: 0

摘要

目的确定从马来西亚半岛七家主要医院的非孕妇成人中分离出的 B 组链球菌（GBS）的流行率；同时调查 GBS 的发病率、病死率、抗菌药敏感性模式、血清型、基因型以及毒力基因和纤毛基因的分布情况。研究方法：这项多中心、前瞻性、观察性研究在马来西亚的七家主要三级医院的非孕妇成人中进行。同时，在选定的医院中以类似的方法进行回顾性研究。对分离出的 GBS 进行表型分析、通过多重 PCR 进行血清分型、抗菌药敏感性测试以及 PCR 检测 GBS 的毒力基因和柔毛基因。对 7 个看家基因进行扩增和测序，以便进行多焦点序列分型。讨论：研究结果有助于临床实践管理，及时诊断和预防 GBS 相关疾病。通过监测抗菌素耐药性，鼓励谨慎使用抗生素。

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Disease burden, antimicrobial resistance and molecular characterization of invasive group B Streptococcus among non-pregnant adults in Malaysia: A protocol study

Objective: To determine the prevalence of group B Streptococcus (GBS) isolated from non-pregnant adults from seven major hospitals in Peninsular Malaysia; the incidence, case-fatality ratio, antimicrobial susceptibility patterns, serotype, genotype and distribution of the GBS virulence and pili genes are also investigated. Methods: This multicentre, prospective, observational study is conducted in seven major tertiary hospitals in Malaysia among non-pregnant adults. Simultaneously, a retrospective study is conducted in the selected hospitals with similar approaches. GBS isolates are subjected to phenotyping, serotyping by multiplex PCR, antimicrobial susceptibility testing and PCR-detection of GBS virulence and pilus genes. Seven housekeeping genes are amplified and sequenced for multi-locus sequence typing. Discussion: Findings from the study may contribute to the management of clinical practice to diagnose and prevent GBS related diseases in a timely manner. Prudent use of antibiotics is encouraged by monitoring antimicrobial resistance.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.