从无包装鸡块中分离出的溶血细菌的检测和致病性水平筛选

Ika Alfiyani, A. Mukaromah, M. Afriansyah, S. Ethica
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摘要

食物中毒是一种可导致死亡的严重威胁。据世界卫生组织称,近年来食物中毒病例呈上升趋势。致病细菌是食物中毒的元凶,通常源于受污染的食物。鸡块是一种广受欢迎的即食食品,由于在储存过程中存在微生物污染的风险,很容易滋生细菌。本研究旨在评估鸡块在室温下存放七天后的细菌致病性水平。在室温下保存的鸡块样本经稀释后在营养琼脂(NA)培养基上进行培养。随后,使用麦康凯琼脂(MC)、血琼脂平板(BAP)和巧克力琼脂平板(CAP)进行细菌致病性筛选。结果发现了六种细菌分离物,标记为 FMCN-1 至 FMCN-6(FMCN 表示 Fatmawati Market 鸡块)。大多数分离物(FMCN-2、FMCN-3、FMCN-4、FMCN-5 和 FMCN-6)具有中等程度的致病性,只有一个分离物(FMCN-1)具有低致病性。总之,鸡块在室温下存放七天会导致细菌污染,致病性大多为中度。需要进一步进行分子分析,以确定潜在的有害细菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
DETECTION AND PATHOGENICITY LEVEL SCREENING OF HEMOLYTIC BACTERIA ISOLATED FROM UNPACKED CHICKEN NUGGET
Food poisoning is a severe threat that can lead to death. According to the WHO, cases of food poisoning have been on the rise in recent years. Pathogenic bacteria are responsible for food poisoning, often originating from contaminated food. Chicken nuggets, a popular ready-to-eat food, are susceptible to bacterial growth due to the risk of microbial contamination during storage. This study aims to assess the pathogenicity levels of bacteria in chicken nuggets when stored at room temperature for seven days. Samples from chicken nuggets left at room temperature were diluted and cultured on Nutrient Agar (NA) media. Subsequently, bacterial pathogenicity screening was conducted using MacConkey Agar (MC), Blood Agar Plate (BAP), and Chocolate Agar Plate (CAP). The results revealed six bacterial isolates labeled FMCN-1 to FMCN-6 (FMCN denoting Fatmawati Market chicken nugget). Most isolates exhibited a moderate level of pathogenicity (FMCN-2, FMCN-3, FMCN-4, FMCN-5, and FMCN-6), with only one isolate demonstrating low pathogenicity (FMCN-1). In conclusion, storing chicken nuggets at room temperature for seven days can lead to bacterial contamination with mostly moderate pathogenicity. Further molecular analysis is required to identify the potentially harmful bacteria.
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