Novel derivative compound produced from carbofuran insecticide degradation and transformation promoted by Pseudomonas fluorescens

Carbofuran insecticide is one of the insecticides most often used by Indonesian farmers. The United Nations Environment Program (UNEP) 2011 in the decision of UNEP/FAO/RC/CRC.11/6, agreed that the active ingredient Carbofuran is dangerous to human health and the environment. P. fluorescens bacteria are known to be able to remediate carbofuran-contaminated soil. This study examines more deeply the biodegradation and biotransformation processes of Carbofuran in P. fluorescens bacteria. The research was carried out at the Laboratory of Plant Diseases, Faculty of Agriculture, Brawijaya University; Analytical Chemistry Laboratory, State Polytechnic of Malang; and Analytical Chemistry Laboratory, Udayana University from February to August 2020. The results showed that P. fluorescens was able to degrade the insecticide Carbofuran in liquid media up to 82 % and the growth of P. fluorescens bacteria reached 2.9 x 10¹² CFU/mL, bacteria P. fluorescens is able to degrade the insecticide Carbofuran in a mixture of soil and compost up to 85 % and the growth of P. fluorescens bacteria reaches 7.5 x 10¹³ CFU/mL, resulting in Carbofuran insecticide derivatives from the biotransformation process, there are 3-hydroxy-7-phenol; 2,3-dihydro-1-benzofuran-2,2,7-triol; (2Z)-2-[(2Z)-pent-2-en-1-ylidene]butane-1,4,4-trio; 7-phenol; 2,2,3-trihydroxy-2,3-dihydro-1-benzofuran-7-yl hydrogen carbonate; 2,2-dimethyl-2,3-dihydro-1-benzofuran-7-yl acetate; 7-(hydroxymethoxy)-2,3-dihydro-1-benzofuran-2,2,3,5-tetrol; 3-hidroksi-2-fenilpropil Carbamat; 2-(3-hydroperoxy-2-hydroxyphenyl)ethane-1,1-diol, 3-keto-7-fenol; 4-hydroxy-2,2-dimethyl-2,3-dihydro-1H-inden-1-one; 3-hydroxy-carbofuran; and 7-methoxy-2,2-dimethyl-2,3-dihydro-1-benzofuran-3,5-diol. Carbofuran derivative compounds from the biodegradation of P. fluorescens bacteria are less toxic than the active ingredient Carbofuran.