利用叶绿体微卫星标记对 Magarach 安培拉克葡萄品种进行 DNA 分型

H. V. Korniliev, V.I. Risovannaya, Ya.Yu. Ryazankina
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引用次数: 0

摘要

该研究首次获得了马加拉赫安培地理学藏品中不同生态和地理类别的 10 个研究葡萄品种的叶绿体 DNA ccmp3、ccmp5 和 ccmp10 位点的微卫星图谱,并确定了它们的叶绿型。西欧品种 "赤霞珠 "和 "黑皮诺 "被用作参照。在所研究的品种中,ccmp3 基因座上的等位基因分别为 106 和 107 bps,ccmp5 基因座上的等位基因分别为 104 和 105 bps,ccmp10 基因座上的等位基因分别为 115 和 116 bps。品种'Genusa Tsibil'以及种间杂交种'Castor'和'Muscat de Yaloven'在ccmp3、ccmp5和ccmp10基因座上的基因型为 106 105 115 bps,相当于叶绿型 B。根据这些基因位点,'Vasarga Chernaya'、'Zaarma'、'Marash Cher-ven'、'Ozirovka'、'Plavai'、'Porsi Shekerek'等品种的基因型为 106 105 116 bps,属于叶绿型 C。根据这些基因位点,品种 "Shirvan Shakhi "的基因型为 107 104 115 bps,相当于叶绿型 D。获得的数据可用于研究育种计划中的品种品系,评估它们之间的关系,还有助于补充葡萄栽培品种 SSR 图谱数据库。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
DNA TYPING OF GRAPE VARIETIES OF MAGARACH AMPELOGRAPHIC COLLECTION USING CHLOROPLASTIC MICROSATELLITE MARKERS
For the first time, microsatellite profiles of chloroplast DNA for ccmp3, ccmp5, ccmp10 loci for 10 studied grape varieties of the Maga-rach Ampelographic Collection of various eco-logical and geographical groups were obtained with determining their chlorotype. Western Eu-ropean varieties ‘Cabernet Sauvignon’ and ‘Pi-not Noir’ were used as a reference. Alleles of 106 and 107 bps were identified at the ccmp3 locus, 104 and 105 bps at the ccmp5 locus, and 115 and 116 bps at the ccmp10 locus in the studied varieties. The variety ‘Genusa Tsibil’ and interspecific hybrids ‘Castor’ and ‘Muscat de Yaloven’ had a genotype of 106 105 115 bps at the ccmp3, ccmp5, ccmp10 loci, which corresponded to the chlorotype B. The varieties ‘Vasarga Chernaya’, ‘Zaarma’, ‘Marash Cher-ven’, ‘Ozirovka’, ‘Plavai’, ‘Porsi Shekerek’ ac-cording to these loci had a genotype of 106 105 116 bps and corresponded to the chloro-type C. The variety ‘Shirvan Shakhi’ according to these loci had a genotype of 107 104 115 bps, corresponding to the chlorotype D. The data obtained can be used in studying the line of varieties in breeding programs, in assessing their relationships, and also can contribute to replenishing the database of SSR profiles of grapevine cultivars.
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