Said K. Taha , Elham A. Hassan , Gehan T. El-Bassyouni , Sahar Mousa , Heba N. Shalash , Mohamed A. Abdel Hamid
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Clinical, radiographic, and histological evaluations were performed for the newly formed bone.</p></div><div><h3>Results</h3><p>Clinical results revealed no infection or inflammatory conditions<strong>.</strong><span> All graft groups revealed a significant increase (P<0.05) in mature bone mean density versus controls. TCP/HA bidoped with Ag and Zr group elucidated a highly significant increase (P <0.01) in mature bone density compared to other groups while the TCP/HA was statistically insignificant (P > 0.05) compared to the Ag-doped TCP/HA. The bactericidal action of both Zr and Ag nanoparticles was evident by antibacterial testing.</span></p></div><div><h3>Conclusion</h3><p><span>Bi-doping of TCP/HA with Ag and Zr has a synergistic osteoconductive effect to enhance bone formation as an excellent modality for defective bone reconstruction. Histomorphometric investigation clarified that Ag and Zr doped TCP/HA stimulated osteogenesis and bone remodeling as compared to un-doped TCP/HA. Therefore, this nanocomposite has promising </span>clinical significance<span> in grafting maxillofacial bone defects for optimal healing compared to un-doped TCP/HA. Moreover, Zr and Ag nanoparticles are effective bactericidal materials with optimal biological safety on the animals.</span></p></div>","PeriodicalId":45034,"journal":{"name":"Journal of Oral and Maxillofacial Surgery Medicine and Pathology","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of osseous healing in induced mandibular defects grafted with biphasic calcium phosphate doped with silver and zirconium nanoparticles: An experimental study in dogs\",\"authors\":\"Said K. Taha , Elham A. Hassan , Gehan T. El-Bassyouni , Sahar Mousa , Heba N. Shalash , Mohamed A. Abdel Hamid\",\"doi\":\"10.1016/j.ajoms.2023.12.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>Evaluation of the osteoconductive p otential of the nanocomposite tricalcium phosphate/hydroxyapatite (TCP/HA) bi-doped with zirconium (Zr) and silver (Ag) ions versus TCP/HA & 4AgTCP/HA in induced defects.</p></div><div><h3>Methods</h3><p>Fifteen dogs were divided into four groups in 12 weeks duration before euthanasia: 1-control, 2- TCP/HA, 3- TCP/HA doped with Ag and 4-TCP/HA bi-doped with Ag & Zr groups. Four surgically created defects were randomly allocated in each animal on the left and right mandibular sides, three defects were grafted with mentioned alloplasts and one empty control. Clinical, radiographic, and histological evaluations were performed for the newly formed bone.</p></div><div><h3>Results</h3><p>Clinical results revealed no infection or inflammatory conditions<strong>.</strong><span> All graft groups revealed a significant increase (P<0.05) in mature bone mean density versus controls. TCP/HA bidoped with Ag and Zr group elucidated a highly significant increase (P <0.01) in mature bone density compared to other groups while the TCP/HA was statistically insignificant (P > 0.05) compared to the Ag-doped TCP/HA. The bactericidal action of both Zr and Ag nanoparticles was evident by antibacterial testing.</span></p></div><div><h3>Conclusion</h3><p><span>Bi-doping of TCP/HA with Ag and Zr has a synergistic osteoconductive effect to enhance bone formation as an excellent modality for defective bone reconstruction. Histomorphometric investigation clarified that Ag and Zr doped TCP/HA stimulated osteogenesis and bone remodeling as compared to un-doped TCP/HA. Therefore, this nanocomposite has promising </span>clinical significance<span> in grafting maxillofacial bone defects for optimal healing compared to un-doped TCP/HA. 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引用次数: 0
摘要
目的评估双掺杂锆（Zr）和银（Ag）离子的纳米复合材料磷酸三钙/羟基磷灰石（TCP/HA）与 TCP/HA & 4AgTCP/HA 在诱导缺损中的骨诱导潜力。方法将 15 只狗在安乐死前分为四组，每组 12 周：1-对照组、2-TCP/HA 组、3-掺杂银离子的 TCP/HA 组和 4-TCP/HA 双掺杂银离子和锆离子组。每只动物的下颌骨左右两侧随机分配了四个手术造成的缺损，其中三个缺损移植了上述异体材料，另一个缺损为空白对照组。对新形成的骨质进行了临床、放射学和组织学评估。与对照组相比，所有移植组的成熟骨平均密度都有显著增加（P<0.05）。与其他组相比，掺入 Ag 和 Zr 的 TCP/HA 组的成熟骨密度有非常明显的增加（P<0.01），而与掺入 Ag 的 TCP/HA 相比，TCP/HA 在统计学上并不显著（P> 0.05）。结论 在 TCP/HA 中掺入 Ag 和 Zr 具有协同骨诱导效应，可促进骨形成，是缺损骨重建的一种极佳方法。组织形态学研究表明，与未掺杂的 TCP/HA 相比，掺杂了 Ag 和 Zr 的 TCP/HA 能刺激骨生成和骨重塑。因此，与未掺杂的 TCP/HA 相比，这种纳米复合材料在移植颌面骨缺损以获得最佳愈合方面具有广阔的临床意义。此外，Zr 和 Ag 纳米粒子是有效的杀菌材料，对动物具有最佳的生物安全性。
Evaluation of osseous healing in induced mandibular defects grafted with biphasic calcium phosphate doped with silver and zirconium nanoparticles: An experimental study in dogs
Objective
Evaluation of the osteoconductive p otential of the nanocomposite tricalcium phosphate/hydroxyapatite (TCP/HA) bi-doped with zirconium (Zr) and silver (Ag) ions versus TCP/HA & 4AgTCP/HA in induced defects.
Methods
Fifteen dogs were divided into four groups in 12 weeks duration before euthanasia: 1-control, 2- TCP/HA, 3- TCP/HA doped with Ag and 4-TCP/HA bi-doped with Ag & Zr groups. Four surgically created defects were randomly allocated in each animal on the left and right mandibular sides, three defects were grafted with mentioned alloplasts and one empty control. Clinical, radiographic, and histological evaluations were performed for the newly formed bone.
Results
Clinical results revealed no infection or inflammatory conditions. All graft groups revealed a significant increase (P<0.05) in mature bone mean density versus controls. TCP/HA bidoped with Ag and Zr group elucidated a highly significant increase (P <0.01) in mature bone density compared to other groups while the TCP/HA was statistically insignificant (P > 0.05) compared to the Ag-doped TCP/HA. The bactericidal action of both Zr and Ag nanoparticles was evident by antibacterial testing.
Conclusion
Bi-doping of TCP/HA with Ag and Zr has a synergistic osteoconductive effect to enhance bone formation as an excellent modality for defective bone reconstruction. Histomorphometric investigation clarified that Ag and Zr doped TCP/HA stimulated osteogenesis and bone remodeling as compared to un-doped TCP/HA. Therefore, this nanocomposite has promising clinical significance in grafting maxillofacial bone defects for optimal healing compared to un-doped TCP/HA. Moreover, Zr and Ag nanoparticles are effective bactericidal materials with optimal biological safety on the animals.
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