研究槲皮素对急性暴露于环磷酰胺的雄性大鼠的潜在肝保护作用

Mustafa M. Khalaf, Rana A Salih
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引用次数: 0

摘要

本研究旨在评估槲皮素在大鼠模型中对环磷酰胺诱导的肝毒性的保肝功效。研究人员将 28 只体重为 195.5 至 198.2 克、年龄约为 3 个月的雄性 Wister 白化大鼠随机分为未注射槲皮素的大鼠和注射槲皮素的大鼠。2 g,年龄约为三个月,被随机分为四个不同的组:未经处理的对照组不接受任何干预措施;CYP 组接受腹腔注射环磷酰胺治疗,剂量为 200 mg/BW;Qt 组接受口服槲皮素治疗,剂量为每天 100 mg/kg BW,为期十天;联合组(Qt+CYP)接受口服槲皮素治疗,为期十天,然后在第十天注射环磷酰胺。除了体重和凝血酶原时间外,还分析了各种生化指标,包括丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、肝脏谷胱甘肽(GSH)和丙二醛(MDA)。未经处理的对照组显示了所有评估指标的基线水平。相比之下,CYP 组的谷丙转氨酶(ALT)、谷草转氨酶(AST)、谷丙转氨酶(ALP)和丙二醛(MDA)水平升高,同时谷胱甘肽(GSH)下降。值得注意的是,与 CYP 组相比,Qt+CYP 组的 ALT、AST、ALP 和 MDA 水平显著降低(P<0.05),GSH 和凝血酶原时间也有所增加。各组体重均无明显差异(P<0.05)。研究结果表明,槲皮素有可能被用作一种肝脏保护剂,保护肝脏组织免受环磷酰胺的细胞毒性影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigating the Potential Hepatoprotective Effect of Quercetin in Male Rats ‎Following Acute Exposure to Cyclophosphamide
This study aimed to assess the hepatoprotective efficacy of quercetin against ‎hepatotoxicity ‎induced by cyclophosphamide in a rat model. A total of 28 male ‎Wister albino rats (Rattus ‎norvegicus), with body ‎weights ranging from 195.5 to ‎‎198.2 g and approximately three months ‎of age, were randomized into four different ‎groups: the untreated Control group ‎received no interventions; the CYP group was treated with an intraperitoneal ‎injection of ‎cyclophosphamide at a dose of 200 mg/BW; the Qt group received an ‎‎oral administration of quercetin at 100 mg/kg BW daily for ten days; and the combined (Qt+CYP) group received quercetin orally for ten days, followed by a ‎cyclophosphamide ‎injection on the tenth day. Various biochemical markers, ‎including alanine aminotransferase ‎‎(ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and liver glutathione ‎‎(GSH), and malondialdehyde ‎‎(MDA), were analyzed, in addition to body weight and ‎prothrombin time. The ‎Untreated Control group exhibited baseline levels for all assessed ‎markers. In ‎contrast, the CYP group showed elevated levels of ALT, AST, ‎‎ALP, and MDA, coupled with a decrease in GSH. Notably, the Qt+CYP ‎group ‎demonstrated a statistically significant reduction (P‎‎<0.05) in ALT, AST, ALP, ‎and MDA levels, ‎as well as an increase in GSH and prothrombin time, when ‎compared to the CYP group. No significant differences in body ‎weight were observed across all groups ‎‎(P‎‎<0.05). The results of the study indicate that quercetin has the potential to be used as a ‎‎hepatoprotective agent, protecting liver tissues from the cytotoxic effects of cyclophosphamide.
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