TOXICITY TEST OF WASTE OIL BEFORE AND AFTER TREATMENT USING PHYTOREMEDIATION ON BIOINDICATORS

Silugonggo River is a river that crosses Juwana District, Pati Regency, Central Java, which empties into the Java Sea. Industrial activities and workshops around the Silugonggo River cause river water to become polluted with used oil.. Even though many people around the river use the Silugonggo River for daily activities, one example is as a place to find fish for side dishes and traded.. The purpose of this study was to determine the concentration of lead in river water contaminated with used oil waste and the LC50 value of 0-96 hours against bioindicators, namely tilapia (Oreochromis niloticus), before and after being processed using phytoremediation. This research phase includes: 1) toxicity tests before treatment consisting of: preliminary tests with waste concentrations of 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100%, and actual tests with waste concentrations of 15, 16, 17, 18, 19, 20, 21, 22, 23, and 24%; 2) The process of treating waste oil with phytoemidiation using water hyacinth and kale plants is tested for plant weight variations. Plant weight variations are 200 grams, 300 grams, 400 grams, and 500 grams, and the process of processing waste oil is carried out continuously; 3) Post-treatment toxicity test consisting of: preliminary test with waste concentrations of 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100%, and actual tests with waste concentrations of 42, 43, 44, 45, 46, 47, 48, 49, 50, and 51%. The results showed that the effective weight in reducing lead in water hyacinth plants and kale plants was 500 grams with lead weight being <0.0058 mg / L (under Quality Standards according to the Decree of the Government Regulation of the Republic of Indonesia Number 82 of 2001 concerning Water Quality Management and Water Pollution Control). The LC50 value of 96 hours of waste oil before processing is 19.66%. The LC50 value 96 hours after processing is 46.69%.