用清除自由基 DPPH 法检测烤柚子(Parkia timoriana)的抗氧化活性

Idmanurrahman Hadi, Teguh Adiyas Putra
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摘要

Kedawung 种子(Parkia timoriana)含有多酚和类黄酮,具有抗氧化等药理作用。在印度尼西亚的一些地区,人们使用焙烧法保存 Kedawung 种子。本研究旨在分析烘焙对植物化学化合物含量的影响,并采用 DPPH 法测定其抗氧化活性。在乙醇中采用浸泡法提取烘焙过的格达旺种子。进行了植物化学筛选,以定性分析提取物中几种植物化学物质的含量。结果表明,焙烤过的 Kedawung 种子提取物中含有生物碱、黄酮类、皂苷和萜类化合物。抗氧化测试采用 DPPH 法,以抗坏血酸为阳性对照。抗坏血酸、P. timoriana 种子和烤过的 P. timoriana 种子的 IC50 值分别为 12.3 ppm、20.6 ppm 和 9.8 ppm,属于强抗氧化化合物。这些结果表明,经过烘烤处理的种子抗氧化效果增强。因此,焙烤过程可以提高 P. timoriana 种子的抗氧化效果:Kedawung 种子、焙烧法、抗氧化剂、DPPH
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ANTIOXIDANT ACTIVITY OF ROASTED KEDAWUNG SEED (Parkia timoriana) USING SCAVENGER FREE RADICAL DPPH METHOD
Kedawung seeds (Parkia timoriana) contain polyphenolics and flavonoids that can provide pharmacological activities, such as antioxidants. In several regions of Indonesia, kedawung seeds are preserved using the roasting method. This study aimed to analyze the effect of roasting on the content of phytochemical compounds and determine their antioxidant activity using the DPPH method. Roasted kedawung seeds were extracted using a maceration method in ethanol. Phytochemical screening was performed to qualitatively analyze the contents of several phytochemical compounds in the extract. The results showed that roasted kedawung seed extract contained alkaloids, flavonoids, saponins, and terpenoids. Antioxidant testing was performed using the DPPH method with ascorbic acid as a positive control. The IC50 value of ascorbic acid, P. timoriana seed and roasted seed of P. timoriana measured each 12.3 ppm; 20.6 ppm and 9.8 ppm; which categorized as strong antioxidant compounds. These results indicated an increase in the antioxidant effect after roasted seed processing. It concluded that the roasting process could enhance antioxidant effect of P. timoriana seed  Keywords: Kedawung seeds, roasted method, antioxidant, DPPH
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