{"title":"利用 UPLC-MS/MS 分析枳椇子和余甘子复合提取物中的目标成分","authors":"Mei Zhang, Hongyuan Zhang, Zhiwei Huang, Yanjie Zhang, Ling liang, Benyong Lou","doi":"10.1166/jbmb.2024.2346","DOIUrl":null,"url":null,"abstract":"This study aimed to establish a UPLC-MS/MS method for rapid analysis and identification of target components in the combination extract of Hovenia dulcis and Phyllanthus emblica. The gradient elution was carried out with methanol (A) −0.2% formic acid solution (A) with low rate of 1.0 mL · min−1, column temperature of 30 °C, detection wavelength of 285 nm. The ESI ion source was used to collect data under the negative ion mode. Through analysis and identification, five target components were inferred, namely gallic acid, dihydromyricetin, corilagine, taxifolin, and ellagic acid in the combination extract. Quantitative analysis was conducted on the combination extract. By utilizing the theory of modern supramolecular technology in traditional Chinese medicine, the functional composite product was obtained by selecting the compound extraction of Hovenia dulcis and Phyllanthus emblica as raw materials, and quantitative analysis was conducted on on the combination extract. The results showed that the flavonoid extraction rate and the content of target components were both higher when the mass ratio of Hovenia dulcis to Phyllanthus emblica was 1:1 and 1:2. This indicates that the co-extraction of Hovenia dulcis and Phyllanthus emblica not only simplified the extraction process but also enhances the extraction rate of the flavonoids in Hovenia dulcis. The developed UPLC-MS/MS method is rapid, sensitive, which provides a technical basis for the selection of raw material mass ratios and product development of Hovenia dulcis and Phyllanthus emblica combination.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":"23 6","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analysis of Targeted Components in the Combination Extract of Hovenia dulcis and Phyllanthus emblica by UPLC-MS/MS\",\"authors\":\"Mei Zhang, Hongyuan Zhang, Zhiwei Huang, Yanjie Zhang, Ling liang, Benyong Lou\",\"doi\":\"10.1166/jbmb.2024.2346\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study aimed to establish a UPLC-MS/MS method for rapid analysis and identification of target components in the combination extract of Hovenia dulcis and Phyllanthus emblica. The gradient elution was carried out with methanol (A) −0.2% formic acid solution (A) with low rate of 1.0 mL · min−1, column temperature of 30 °C, detection wavelength of 285 nm. The ESI ion source was used to collect data under the negative ion mode. Through analysis and identification, five target components were inferred, namely gallic acid, dihydromyricetin, corilagine, taxifolin, and ellagic acid in the combination extract. Quantitative analysis was conducted on the combination extract. By utilizing the theory of modern supramolecular technology in traditional Chinese medicine, the functional composite product was obtained by selecting the compound extraction of Hovenia dulcis and Phyllanthus emblica as raw materials, and quantitative analysis was conducted on on the combination extract. The results showed that the flavonoid extraction rate and the content of target components were both higher when the mass ratio of Hovenia dulcis to Phyllanthus emblica was 1:1 and 1:2. This indicates that the co-extraction of Hovenia dulcis and Phyllanthus emblica not only simplified the extraction process but also enhances the extraction rate of the flavonoids in Hovenia dulcis. The developed UPLC-MS/MS method is rapid, sensitive, which provides a technical basis for the selection of raw material mass ratios and product development of Hovenia dulcis and Phyllanthus emblica combination.\",\"PeriodicalId\":15157,\"journal\":{\"name\":\"Journal of Biobased Materials and Bioenergy\",\"volume\":\"23 6\",\"pages\":\"\"},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Biobased Materials and Bioenergy\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1166/jbmb.2024.2346\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biobased Materials and Bioenergy","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1166/jbmb.2024.2346","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
本研究旨在建立一种UPLC-MS/MS方法,用于快速分析和鉴定枳椇子和白花蛇舌草提取物中的目标成分。采用甲醇(A)-0.2%甲酸溶液(A)进行梯度洗脱,流速为1.0 mL - min-1,柱温为30 ℃,检测波长为285 nm。在负离子模式下使用 ESI 离子源采集数据。通过分析和鉴定,推断出组合提取物中含有 5 种目标成分,即没食子酸、二氢杨梅素、柯里拉京、紫杉叶素和鞣花酸。对组合提取物进行了定量分析。利用现代中药超分子技术理论,选择枳壳和白术为原料进行复合提取,得到了功能性复合产品,并对复合提取物进行了定量分析。结果表明,当枳椇子与白花蛇舌草的质量比为 1:1 和 1:2 时,黄酮提取率和目标成分含量均较高。这表明枳椇子和白皮共萃取不仅简化了萃取过程,而且提高了枳椇子中黄酮类化合物的萃取率。所建立的UPLC-MS/MS方法快速、灵敏,为枳椇子和白皮的原料质量比选择和产品开发提供了技术依据。
Analysis of Targeted Components in the Combination Extract of Hovenia dulcis and Phyllanthus emblica by UPLC-MS/MS
This study aimed to establish a UPLC-MS/MS method for rapid analysis and identification of target components in the combination extract of Hovenia dulcis and Phyllanthus emblica. The gradient elution was carried out with methanol (A) −0.2% formic acid solution (A) with low rate of 1.0 mL · min−1, column temperature of 30 °C, detection wavelength of 285 nm. The ESI ion source was used to collect data under the negative ion mode. Through analysis and identification, five target components were inferred, namely gallic acid, dihydromyricetin, corilagine, taxifolin, and ellagic acid in the combination extract. Quantitative analysis was conducted on the combination extract. By utilizing the theory of modern supramolecular technology in traditional Chinese medicine, the functional composite product was obtained by selecting the compound extraction of Hovenia dulcis and Phyllanthus emblica as raw materials, and quantitative analysis was conducted on on the combination extract. The results showed that the flavonoid extraction rate and the content of target components were both higher when the mass ratio of Hovenia dulcis to Phyllanthus emblica was 1:1 and 1:2. This indicates that the co-extraction of Hovenia dulcis and Phyllanthus emblica not only simplified the extraction process but also enhances the extraction rate of the flavonoids in Hovenia dulcis. The developed UPLC-MS/MS method is rapid, sensitive, which provides a technical basis for the selection of raw material mass ratios and product development of Hovenia dulcis and Phyllanthus emblica combination.