从薯蓣中提取薯蓣皂苷的清洁工艺

IF 0.5 4区 医学
Jinwei Liu, Weichang Xu, Feng Yan, Xinqi Liu, Yuan Wang
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引用次数: 0

摘要

这项研究采用高效液相色谱和液相色谱-质谱(LC-MS)技术,阐明了通过清洁工艺获得的薯蓣皂苷的酶解和酸水解机制。研究结果表明,酶解导致原薯蓣皂苷 C-26 位糖苷键的裂解和随后的重组,从而在酶水解过滤残留物中形成薯蓣皂苷。根据这一观察结果,我们设计出了一种提取薯蓣皂苷的简化且环保的方法。通过箱-贝肯响应面方法和废水评估,确定了清洗工艺的最佳参数:硫酸浓度为 3 mol - L-1,固液比为 1:10,酸水解温度为 100 °C,酸水解持续时间为 3 小时。与直接酸水解法相比,该方法的薯蓣皂苷产率提高了 133.08%,薯蓣皂苷纯度提高了 44.08%,废水产生量和酸利用率降低了 50%,废水的化学需氧量(COD)降低了 83.57%。这种优化的清洁工艺可用于大规模生产,并提供了一种可持续的薯蓣皂苷生产方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cleaning Process of Diosgenin from Dioscorea nipponica
This investigation employed HPLC and LC-MS techniques to elucidate the enzymolysis and acid hydrolysis mechanisms of diosgenin obtained through a cleaning process. The findings revealed that the enzymolysis led to the cleavage and subsequent recombination of the glycosidic bond at the C-26 position of protodioscin, resulting in the formation of dioscin present in the enzymatic hydrolysis filter residue. Leveraging this observation, a streamlined and eco-friendly method for diosgenin extraction was devised. Incorporating the Box-Behnken response surface methodology alongside wastewater assessment, the optimal parameters for the cleaning process were established: a sulfuric acid concentration of 3 mol · L−1, a solid–liquid ratio of 1:10, an acid hydrolysis temperature of 100 °C, and an acid hydrolysis duration of 3 h. Under these parameters, the yield and purity of diosgenin were 31.07±0.56% and 72.30±0.24% respectively. When benchmarked against the direct acid hydrolysis approach, there was an increase of 133.08% in diosgenin yield, 44.08% enhancement in diosgenin purity, 50% reduction in wastewater generation and acid utilization, and an 83.57% decrease in wastewater’s chemical oxygen demand (COD). This optimized cleaning process is viable for large-scale production and offers a sustainable method for diosgenin production.
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来源期刊
Journal of Biobased Materials and Bioenergy
Journal of Biobased Materials and Bioenergy 工程技术-材料科学:生物材料
自引率
0.00%
发文量
60
审稿时长
6 months
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