比较代谢组学揭示了黑桑树和白桑树在不同生长阶段生物碱代谢的差异

IF 0.5 4区 医学
Lu Yang, Shaoli Fan, Jiuyang Zhao, Jinfa Liao, Yicun Chen, Yangdong Wang
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引用次数: 0

摘要

桑树(Morus spp.)是桑科的一种开花植物,因其果实可食用而闻名,桑叶也有多种用途。本实验利用超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF-MS)和 Progenesis QI 软件快速鉴定黑桑树和白桑树叶片中的次生代谢物。利用正交偏最小二乘判别分析(OPLS-DA)、主成分分析(PCA)和层次聚类分析(HCA)研究了黑桑叶和白桑叶在七个初级生长阶段中代谢物(尤其是生物碱)的动态变化和阶段特异性。结果显示,不同生长阶段的叶片样本共发现了 13 个不同类别的 182 种代谢物。根据叶片代谢物积累模式的差异,黑麦草的代谢物含量明显高于白麦草。多重统计分析显示,黑麦草和白麦草叶片的代谢组表现出显著的种间和发育特异性积累模式,其中黑麦草叶片中大多数 DNJ 生物碱含量最高。在不同的生长阶段,黑麦草叶片的代谢物表现出明显的阶段特异性。在小果期的发育阶段,黑茶叶片的化学成分发生了明显的变化,DNJ 生物碱代谢物的含量显著增加。总之,本研究为新疆黑木耳资源的综合开发和可持续利用提供了理论参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative Metabolomics Revealed Differences in Alkaloids Metabolism Between Morus nigra L. and Morus. alba L. at Different Growth Stages
Mulberry (Morus spp.), a flowering plant in the Moraceae family, is known for the edible fruit, and mulberry leaves have various applications. This experiment utilized ultra-high performance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS) and Progenesis QI software to rapidly identify secondary metabolites in the leaves of Morus nigra L. and Morus alba L.. Orthogonal partial least squares discriminant analysis (OPLS-DA), principal component analysis (PCA) and Hierarchical clustering analysis (HCA) were used to examine the dynamic changes and stage specificity of metabolites, particularly alkaloids, in M. nigra and M. alba leaves during seven primary growth stages. In results, the leaf samples from different growth stages uncovered a total of 182 metabolites from 13 distinct categories. M. nigra had significantly higher levels of metabolites than those in M. alba, according to differences in leaf metabolite accumulation patterns. Multiple statistical analyses revealed that the metabolome of M. nigra and M. alba leaves exhibited a significant interspecific and developmental specific accumulation patterns, with most DNJ alkaloids having the highest content in M. nigra leaves. In different growth stages, the metabolites of M. nigra leaves showed significant stage specificity. During the development stage of small fruit stage, obvious changes occurred in the chemical composition of M. nigra leaves, with a significant increase in DNJ alkaloid metabolites. Overall, this study provides a theoretical reference for the comprehensive development and sustainable utilization of M. nigra resources in Xinjiang.
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来源期刊
Journal of Biobased Materials and Bioenergy
Journal of Biobased Materials and Bioenergy 工程技术-材料科学:生物材料
自引率
0.00%
发文量
60
审稿时长
6 months
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