利用云南微球菌 GKSM13 促进水稻秧苗在铜胁迫下的生长并阐明其基因组性状

IF 5.4 Q1 PLANT SCIENCES
Krishnendu Majhi , Moitri Let , Rajib Bandopadhyay
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引用次数: 0

摘要

水稻在全球范围内广泛种植,有益菌群对可持续农业尤为重要,因为它们可以防止过量的重金属污染,从而促进生长和产量。本研究旨在利用耐铜植物生长促进菌(PGPB)云南微球菌 GKSM13 来改善水稻幼苗(MTU1010 品种)在铜胁迫下的生长状况。菌株 GKSM13 能够产生吲哚-3-乙酸(IAA)、赤霉素 A3(GA3)和氨等植物生长促进因子(PGPFs),积累脯氨酸,固定 N2,抑制 2,2-二苯基-1-苦基肼(DPPH)。经 Cu2+ 处理并与 GKSM13 共同接种的水稻秧苗在形态和生化方面的生长情况都有明显改善。用场发射扫描电子显微镜(FE-SEM)分析发现,GKSM13 以大量茧状细胞的形式与根细胞结合在一起。在 GKSM13 的存在下,水稻秧苗对 Cu2+ 的吸收率降低到 57.5%。菌株 GKSM13 还能激活超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、抗坏血酸过氧化物酶 (APOX) 和谷胱甘肽过氧化物酶 (GPOX) 等抗氧化酶,从而降低 Cu 诱导的水稻秧苗氧化应激。对菌株 GKSM13 基因组的深入研究表明,该菌株含有色氨酸(trp)、ent-kaurene、氰酶(cyn)、磷酸盐特异性转运(pst)、主要促进剂超家族转运体(MFS)、硫酸盐转运体(cys)、脯氨酸(pro)和 SOD(sod)基因,这些基因具有促进植物生长和缓解 Cu2+ 胁迫的作用。因此,应用云南滇金丝猴 GKSM13 菌株可为受铜影响的矿区提供可持续的农业解决方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Efficacious use of Micrococcus yunnanensis GKSM13 for the growth of rice seedlings under copper stress with elucidation into genomic traits

Rice is widely cultivated worldwide, and beneficial bactrial colonization are particularly desirable for sustainable agriculture because they promote growth and production by preventing excessive heavy metal contamination. The present study was conducted with the aim of improving the growth of rice seedlings (MTU1010 variety) under copper (Cu) stress by using Cu-tolerant plant growth promoting bacteria (PGPB) Micrococcus yunnanensis GKSM13. Strain GKSM13 was able to produce plant growth promoting factors (PGPFs) such as indole-3-acetic acid (IAA), gibberellin A3 (GA3) and ammonia, accumulate proline, fix N2, and inhibit 2,2-Diphenyl-1-picrylhydrazyl (DPPH). Rice seedlings treated with Cu2+ and co-inoculated with GKSM13 significantly improved their growth in morphological and biochemical aspects. When analysed by field emission scanning electron microscope (FE-SEM), GKSM13 was found to be associated with the root cells in the form of large number of coccoid cells. The uptake of Cu2+ in rice seedlings was reduced to 57.5% in the presence of GKSM13. Strain GKSM13 treatment also reduced Cu-induced oxidative stress of rice seedlings by activating antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APOX) and glutathione peroxidase (GPOX), which was supported by DPPH inhibition and reduction of malondialdehyde (MDA) accumulation. Insight into the genome of strain GKSM13 reveals the presence of tryptophan (trp), ent-kaurene, cyanase (cyn), phosphate-specific transport (pst), major facilitator superfamily transporter (MFS), sulphate transporter (cys), proline (pro) and SOD (sod) genes, which are responsible for promoting plant growth and alleviating Cu2+ stress. Therefore, the application of strain M. yunnanensis GKSM13 could provide a sustainable agricultural solution for Cu-affected mining areas.

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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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