Zaki A. Siddiqui, Manzoor R. Khan, Sumaiya Aziz, Aqib
{"title":"使用纳米氧化锰颗粒(MnO2 NPs)和假单胞菌(Pseudomonas putida)防治胡萝卜枯萎病。","authors":"Zaki A. Siddiqui, Manzoor R. Khan, Sumaiya Aziz, Aqib","doi":"10.1016/j.exppara.2024.108698","DOIUrl":null,"url":null,"abstract":"<div><p>Wilt disease complex of carrot is caused by <span><em>Ralstonia</em><em> solancearum</em></span> and <span><em>Meloidogyne incognita</em></span> and is responsible for considerable yield loss. Manganese oxide nanoparticle (MnO<sub>2</sub> NPs) and <span><em>Pseudomonas putida</em></span> were used alone and in combination for the management of wilt disease complex. <em>In vitro</em>, MnO<sub>2</sub> NPs 0.10 g.L<sup>-l</sup> caused 49.36% reduction in hatching and 14.23% mortality of second stage juveniles (J<sub>2</sub>) of <em>M. incognita</em> while paper disc dipped in MnO<sub>2</sub><span> NPs suspension caused 0.51 mm inhibition zone around </span><em>R. solanacearum</em><span> in nutrient agar medium. Inoculation of </span><em>P. putida</em> to plants with pathogens caused a similar increase in plant growth, chlorophyll and carotenoid contents as caused by foliar spray with 0.10 g.L<sup>−1</sup> MnO<sub>2</sub> NPs. Use of <em>P. putida</em> with NPs foliar spray to plants with pathogens caused a greater increase in plant growth, chlorophyll and carotenoid contents than with <em>P. putida</em> or NPs foliar spray. Inoculation of <em>M. incognita</em>/<em>R. solanacearum</em>/<em>M. incognita</em> plus <em>R. solanacearum/P. putida/</em>MnO<sub>2</sub> NPs and MnO<sub>2</sub> NPs plus <em>P. putida</em> caused increase in proline contents. Root colonization by <em>P. putida</em> was reduced in plants with test pathogens. Foliar application of MnO<sub>2</sub> NPs and <em>P. putida</em> reduce wilt disease indices. Galling and populations of <em>M. incognita</em> was also reduced in plants co-inoculated with <em>R. solanacearum</em>. The greatest reduction in nematode populations and galling was observed in plants with NPs spray together with <em>P. putida</em>. Principal component analysis demonstrated a clear influence of NPS and <em>P. putida</em> and their combination on various studied parameters in diseased plants.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Use of manganese oxide nanoparticle (MnO2 NPs) and Pseudomonas putida for the management of wilt disease complex of carrot\",\"authors\":\"Zaki A. Siddiqui, Manzoor R. Khan, Sumaiya Aziz, Aqib\",\"doi\":\"10.1016/j.exppara.2024.108698\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Wilt disease complex of carrot is caused by <span><em>Ralstonia</em><em> solancearum</em></span> and <span><em>Meloidogyne incognita</em></span> and is responsible for considerable yield loss. Manganese oxide nanoparticle (MnO<sub>2</sub> NPs) and <span><em>Pseudomonas putida</em></span> were used alone and in combination for the management of wilt disease complex. <em>In vitro</em>, MnO<sub>2</sub> NPs 0.10 g.L<sup>-l</sup> caused 49.36% reduction in hatching and 14.23% mortality of second stage juveniles (J<sub>2</sub>) of <em>M. incognita</em> while paper disc dipped in MnO<sub>2</sub><span> NPs suspension caused 0.51 mm inhibition zone around </span><em>R. solanacearum</em><span> in nutrient agar medium. Inoculation of </span><em>P. putida</em> to plants with pathogens caused a similar increase in plant growth, chlorophyll and carotenoid contents as caused by foliar spray with 0.10 g.L<sup>−1</sup> MnO<sub>2</sub> NPs. Use of <em>P. putida</em> with NPs foliar spray to plants with pathogens caused a greater increase in plant growth, chlorophyll and carotenoid contents than with <em>P. putida</em> or NPs foliar spray. Inoculation of <em>M. incognita</em>/<em>R. solanacearum</em>/<em>M. incognita</em> plus <em>R. solanacearum/P. putida/</em>MnO<sub>2</sub> NPs and MnO<sub>2</sub> NPs plus <em>P. putida</em> caused increase in proline contents. Root colonization by <em>P. putida</em> was reduced in plants with test pathogens. Foliar application of MnO<sub>2</sub> NPs and <em>P. putida</em> reduce wilt disease indices. Galling and populations of <em>M. incognita</em> was also reduced in plants co-inoculated with <em>R. solanacearum</em>. The greatest reduction in nematode populations and galling was observed in plants with NPs spray together with <em>P. putida</em>. Principal component analysis demonstrated a clear influence of NPS and <em>P. putida</em> and their combination on various studied parameters in diseased plants.</p></div>\",\"PeriodicalId\":12117,\"journal\":{\"name\":\"Experimental parasitology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-01-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental parasitology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0014489424000018\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental parasitology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0014489424000018","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
胡萝卜枯萎病复合体是由 Ralstonia solancearum 和 Meloidogyne incognita 引起的,造成相当大的产量损失。纳米氧化锰(MnO2 NPs)和假单胞菌(Pseudomonas putida)被单独或联合用于防治这种枯萎病。在体外,0.10 g.L-l 的 MnO2 NPs 可使 M. incognita 第二阶段幼虫(J2)的孵化率降低 49.36%,死亡率降低 14.23%,而在营养琼脂培养基中,蘸有 MnO2 NPs 悬浮液的纸盘可在 R. solanacearum 周围形成 0.51 mm 的抑制区。将 P. putida 接种到带有病原体的植物上,与叶面喷洒 0.10 g.L-1 MnO2 NPs 所引起的植物生长、叶绿素和类胡萝卜素含量的增加相似。在有病原体的植物上使用 P. putida 和 NPs 叶面喷洒,比使用 P. putida 或 NPs 叶面喷洒更能提高植物的生长、叶绿素和类胡萝卜素含量。接种 M. incognita/R. solanacearum/M. incognita 加 R. solanacearum/P. putida/MnO2 NPs 和 MnO2 NPs 加 P. putida 会导致脯氨酸含量增加。在有病原体试验的植物中,腐生菌的根定植减少。叶面施用 MnO2 NPs 和 P. putida 可降低枯萎病指数。与 R. solanacearum 共同接种的植株也减少了 M. incognita 的瘿虫和种群数量。在喷洒 NPs 和 P. putida 的植株中,线虫数量和瘿瘤的减少幅度最大。主成分分析表明,NPS 和 P. putida 以及它们的组合对病株的各种研究参数有明显的影响。
Use of manganese oxide nanoparticle (MnO2 NPs) and Pseudomonas putida for the management of wilt disease complex of carrot
Wilt disease complex of carrot is caused by Ralstonia solancearum and Meloidogyne incognita and is responsible for considerable yield loss. Manganese oxide nanoparticle (MnO2 NPs) and Pseudomonas putida were used alone and in combination for the management of wilt disease complex. In vitro, MnO2 NPs 0.10 g.L-l caused 49.36% reduction in hatching and 14.23% mortality of second stage juveniles (J2) of M. incognita while paper disc dipped in MnO2 NPs suspension caused 0.51 mm inhibition zone around R. solanacearum in nutrient agar medium. Inoculation of P. putida to plants with pathogens caused a similar increase in plant growth, chlorophyll and carotenoid contents as caused by foliar spray with 0.10 g.L−1 MnO2 NPs. Use of P. putida with NPs foliar spray to plants with pathogens caused a greater increase in plant growth, chlorophyll and carotenoid contents than with P. putida or NPs foliar spray. Inoculation of M. incognita/R. solanacearum/M. incognita plus R. solanacearum/P. putida/MnO2 NPs and MnO2 NPs plus P. putida caused increase in proline contents. Root colonization by P. putida was reduced in plants with test pathogens. Foliar application of MnO2 NPs and P. putida reduce wilt disease indices. Galling and populations of M. incognita was also reduced in plants co-inoculated with R. solanacearum. The greatest reduction in nematode populations and galling was observed in plants with NPs spray together with P. putida. Principal component analysis demonstrated a clear influence of NPS and P. putida and their combination on various studied parameters in diseased plants.
期刊介绍:
Experimental Parasitology emphasizes modern approaches to parasitology, including molecular biology and immunology. The journal features original research papers on the physiological, metabolic, immunologic, biochemical, nutritional, and chemotherapeutic aspects of parasites and host-parasite relationships.