年龄和饮食限制可调节雄性小鼠股四头肌线粒体的质量。

IF 4.4 4区 医学 Q1 GERIATRICS & GERONTOLOGY
Biogerontology Pub Date : 2024-06-01 Epub Date: 2024-01-06 DOI:10.1007/s10522-023-10086-3
Ting-Rui Zhang, Chun-Hsien Chiang, Tzu-Chieh Hsu, Chih-Yun Wang, Ching-Yi Chen
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引用次数: 0

摘要

饮食限制(DR)是一种潜在的干预措施,可改善与衰老相关的损害。线粒体质量控制是调节骨骼肌细胞功能的关键机制。本研究旨在探讨年龄和 DR 对骨骼肌线粒体质量控制平衡的影响。为了研究年龄对线粒体稳态的影响,研究人员给幼年(3 个月大)雄性 C57BL/6J 小鼠自由喂养(AL)至 7 个月大(幼年)、14 个月大(中年)和 19 个月大(老年)。在 DR 干预中,小鼠在 3 月龄时摄入 60% 的 AL,直至 19 月龄(16 个月)。收集股四头肌进行进一步分析。在中年和老年的过渡阶段,骨骼肌发生了显著变化。中年以后,肌肉中的胶原蛋白开始积累。与中年肌肉相比,老年肌肉中 VDAC 的表达量更高,线粒体动态蛋白和 OXPHOS 蛋白的表达量较低。DR干预减少了衰老过程中骨骼肌中胶原蛋白的含量,并延长了肌节长度。此外,DR 还能调整衰老肌肉线粒体形态的异常。DR 下调了 VDAC 的表达,但上调了 OPA1 和 DRP1 的表达。综上所述,骨骼肌在衰老过程中,尤其是在中年和老年之间的过渡时期,发生了更大的病理变化,而早发性DR通过使线粒体的部分功能正常化,减轻了肌肉的衰老。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Age and dietary restriction modulate mitochondrial quality in quadriceps femoris muscle of male mice.

Age and dietary restriction modulate mitochondrial quality in quadriceps femoris muscle of male mice.

Dietary restriction (DR) is a potential intervention for ameliorating ageing-related damages. Mitochondrial quality control is the key mechanism for regulating cellular functions in skeletal muscle. This study aimed to explore the effect of age and DR on the homeostasis of mitochondrial quality control in skeletal muscle. To study the effect of age on mitochondrial homeostasis, young (3 months old) male C57BL/6J mice were fed ad libitum (AL) until 7 (Young), 14 (Middle), and 19 months (Aged) of age. For the DR intervention, 60% of AL intake was given to the mice at 3 months of age until they reached 19 months of age (16 months). The quadriceps femoris muscle was collected for further analysis. Significant changes in the skeletal muscle were noticed during the transition between middle age and the elderly stages. An accumulation of collagen was observed in the muscle after middle age. Compared with the Middle muscle, Aged muscle displayed a greater expression of VDAC, and lower expressions of mitochondrial dynamic proteins and OXPHOS proteins. The DR intervention attenuated collagen content and elongated the sarcomere length in the skeletal muscle during ageing. In addition, DR adjusted the abnormalities in mitochondrial morphology in the Aged muscle. DR downregulated VDAC expression, but upregulated OPA1 and DRP1 expressions. Taken together, greater pathological changes were noticed in the skeletal muscle during ageing, especially in the transition between middle age and the elderly, whereas early-onset DR attenuated the muscular ageing via normalising partial functions of mitochondria.

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来源期刊
Biogerontology
Biogerontology 医学-老年医学
CiteScore
8.00
自引率
4.40%
发文量
54
审稿时长
>12 weeks
期刊介绍: The journal Biogerontology offers a platform for research which aims primarily at achieving healthy old age accompanied by improved longevity. The focus is on efforts to understand, prevent, cure or minimize age-related impairments. Biogerontology provides a peer-reviewed forum for publishing original research data, new ideas and discussions on modulating the aging process by physical, chemical and biological means, including transgenic and knockout organisms; cell culture systems to develop new approaches and health care products for maintaining or recovering the lost biochemical functions; immunology, autoimmunity and infection in aging; vertebrates, invertebrates, micro-organisms and plants for experimental studies on genetic determinants of aging and longevity; biodemography and theoretical models linking aging and survival kinetics.
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